Abstract
Abstract Comparative field performance of thornless blackberry plants produced by tissue-culture (TC) and standard (ST) methods (tip layers or stem cuttings) was studied to determine any effect of TC propagation on clonal phenotypic stability. In general, TC plants performed as well as, and were phenotypically similar to, ST plants. Some differences in leaf size and pattern were observed; however, the differences were related to the increased vigor of TC plants of these cultivars. Total growth of TC and ST plants of ‘Black Satin’, ‘Hull Thornless’, ‘Thornfree’, and SI-US 68-6-17 was similar. ST plants of ‘Dirksen Thornless’ and ‘Smoothstem’ failed to grow as rapidly as TC plants of these cultivars or ST plants of the other cultivars, resulting in significantly reduced 2nd-year yields. First-year growth of all TC plants was more uniform than corresponding ST plants. On the average, fruit size was smaller on TC plants; however, this was true in both years only for ‘Black Satin’. The response varied for other cultivars with TC plants of ‘Dirksen Thornless’ actually having larger fruits in one year. No consistent differences in bloom date or median harvest date due to propagation method were observed. No variant plants were observed in the field; however, one sectorial leaf chlorotic variant was observed after TC propagation but prior to field planting.
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Claims 12
Claim · model-written summary
Tissue culture plants generally performed as well as standard propagated plants.
Verbatim
e0“TC plants performed as well as, and were phenotypically similar to, ST plants”
t0“Thornless Blackberries”
Claim · model-written summary
Tissue culture plants were phenotypically similar to standard propagated plants.
Verbatim
e0“TC plants performed as well as, and were phenotypically similar to, ST plants”
t0“Thornless Blackberries”
Claim · model-written summary
Leaf size and pattern differed in relation to greater tissue culture plant vigor.
Verbatim
e0“Some differences in leaf size and pattern were observed; however, the differences were related to the increased vigor of TC plants of these cultivars.”
t0“Thornless Blackberries”
Claim · model-written summary
Total growth was similar between propagation methods for four named cultivars.
Verbatim
e0“Total growth of TC and ST plants of ‘Black Satin’, ‘Hull Thornless’, ‘Thornfree’, and SI-US 68-6-17 was similar.”
t0“Thornless Blackberries”
Claim · model-written summary
Standard propagated Dirksen Thornless and Smoothstem grew more slowly and had significantly reduced second year yields.
Verbatim
e0“ST plants of ‘Dirksen Thornless’ and ‘Smoothstem’ failed to grow as rapidly as TC plants of these cultivars or ST plants of the other cultivars, resulting in significantly reduced 2nd-year yields.”
t0“Thornless Blackberries”
Claim · model-written summary
First year growth of tissue culture plants was more uniform.
Verbatim
e0“First-year growth of all TC plants was more uniform than corresponding ST plants.”
t0“Thornless Blackberries”
Claim · model-written summary
Fruit size averaged smaller on tissue culture plants.
Not reportedComparator
Verbatim
e0“On the average, fruit size was smaller on TC plants”
t0“Thornless Blackberries”
Claim · model-written summary
Black Satin tissue culture fruit was smaller in both years.
Verbatim
e0“this was true in both years only for ‘Black Satin’”
t0“Thornless Blackberries”
Claim · model-written summary
Dirksen Thornless tissue culture fruit was larger in one year.
Verbatim
e0“TC plants of ‘Dirksen Thornless’ actually having larger fruits in one year”
t0“Thornless Blackberries”
Claim · model-written summary
Bloom and median harvest dates did not differ consistently by propagation method.
Verbatim
e0“No consistent differences in bloom date or median harvest date due to propagation method were observed.”
t0“Thornless Blackberries”
Claim · model-written summary
No variant plants were observed in the field.
Verbatim
e0“No variant plants were observed in the field”
t0“Thornless Blackberries”
Claim · model-written summary
One sectorial chlorotic leaf variant occurred after tissue culture before planting.
Verbatim
e0“one sectorial leaf chlorotic variant was observed after TC propagation but prior to field planting”
t0“Thornless Blackberries”