Paper · 2022 · The Pharma Innovation

Application of chemicals and nutrients differs in bacterial titre of Candidatus Liberibacter asiaticus on infected Citrus sinensis (L.) Osbeck

Siva Roopa Kumar KSD; Gopal K; Rajasekharam T; et al.

4
claims
7
verbatim quotes
2
citations
3
research areas
✓
Open access

Abstract

Huanglongbing (HLB), or citrus greening disease, is significant havoc to the citrus cultivation that influences citrus industry decline in India and elsewhere. HLB is a systemic, fastidious, phloem inhabited alpha-proteobacterial disease caused by ‘Candidatus Liberibacter asiaticus’ (CLas). Currently, there is no established cure for this emerging but century-old disease. The present study discussed change in cycle threshold (Ct) values of CLas bacterium using qPCR diagnostic method upon applying antibiotics and micronutrients to HLB infected sweet orange cv. Sathgudi plants. Five antibiotics and three micronutrients were selected and applied as a foliar spray in 3 different spraying periods. Results demonstrated that ampicillin sodium (Ct=25.41) and rifampicin (Ct=23.62) showed a significant effect on the suppression of bacterial titre compared to other selected antibiotics at 90 days after the first spray. However, there was no significant effect on the bacterial population regarding its titre and symptom expression when any of the selected micronutrients were applied in any of the spraying periods. Hence, the reported antibiotics could help in the management of an infected HLB citrus orchard at the expense of minimizing total yield loss when they are judiciously used.

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Claims 4

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Ampicillin sodium significantly suppressed CLas bacterial titre compared with other selected antibiotics at 90 days after the first spray.

Taxon
sweet orangeCitrus sinensise0
Material
cv. Sathgudie0
Treatment
ampicillin sodiume1
Interactor
CLase0pathogen
Outcome
bacterial titree1
Direction
suppressione1
Value
Ct=25.41e1
Comparator
other selected antibioticse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus sinensis
Citrus sinensis · TaxonEllen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“change in cycle threshold (Ct) values of CLas bacterium using qPCR diagnostic method upon applying antibiotics and micronutrients to HLB infected sweet orange cv. Sathgudi plants”

✓ located verbatim in the source · abstract, characters 403–581 · show in text ↑
e1

“ampicillin sodium (Ct=25.41) and rifampicin (Ct=23.62) showed a significant effect on the suppression of bacterial titre compared to other selected antibiotics at 90 days after the first spray”

✓ located verbatim in the source · abstract, characters 728–920 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Rifampicin significantly suppressed CLas bacterial titre compared with other selected antibiotics at 90 days after the first spray.

Taxon
sweet orangeCitrus sinensise0
Material
cv. Sathgudie0
Treatment
rifampicine1
Interactor
CLase0pathogen
Outcome
bacterial titree1
Direction
suppressione1
Value
Ct=23.62e1
Comparator
other selected antibioticse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus sinensis
Citrus sinensis · TaxonEllen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“change in cycle threshold (Ct) values of CLas bacterium using qPCR diagnostic method upon applying antibiotics and micronutrients to HLB infected sweet orange cv. Sathgudi plants”

✓ located verbatim in the source · abstract, characters 403–581 · show in text ↑
e1

“ampicillin sodium (Ct=25.41) and rifampicin (Ct=23.62) showed a significant effect on the suppression of bacterial titre compared to other selected antibiotics at 90 days after the first spray”

✓ located verbatim in the source · abstract, characters 728–920 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Selected micronutrients had no significant effect on CLas bacterial population titre or symptom expression in any spraying period.

Taxon
sweet orangeCitrus sinensise0
Treatment
selected micronutrientse2
Interactor
CLase0pathogen
Outcome
bacterial population regarding its titre and symptom expressione2
Direction
no_changeinferrede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus sinensis
Citrus sinensis · TaxonEllen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“change in cycle threshold (Ct) values of CLas bacterium using qPCR diagnostic method upon applying antibiotics and micronutrients to HLB infected sweet orange cv. Sathgudi plants”

✓ located verbatim in the source · abstract, characters 403–581 · show in text ↑
e2

“there was no significant effect on the bacterial population regarding its titre and symptom expression when any of the selected micronutrients were applied in any of the spraying periods”

✓ located verbatim in the source · abstract, characters 931–1117 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Antibiotics could help manage an infected HLB citrus orchard, minimizing total yield loss when they are judiciously used.

Taxon
citrusCitruse3
Treatment
antibioticse3
Outcome
total yield losse3
Qualifier
when they are judiciously usede3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“Hence, the reported antibiotics could help in the management of an infected HLB citrus orchard at the expense of minimizing total yield loss when they are judiciously used”

✓ located verbatim in the source · abstract, characters 1119–1290 · show in text ↑

micronutrient · molecular: expression · Citrus sinensis (sweet orange)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.