Abstract
Sweet potato is a major root crop with nutritious tuberous roots. The mechanism of tuberous root development has not yet been adequately elucidated. Genetic resources are required to develop the molecular understanding of sweet potato. Heavy-ion beams were applied to hexaploid sweet potato for an increase in genetic variation, after which the comprehensive effects of heavy-ion beam irradiation were investigated. In vitro cultured shoots with an axillary bud of 'Beniharuka' were irradiated with Ar-ions at a dose of 1-5 Gy and C-ions at a dose of 5-20 Gy, and three irradiated lines were separated from each irradiated shoot. The shoot regeneration was inhibited at high doses of each ion irradiation. Ar-ion irradiation had an especially high biological effect on shoot regeneration. A total of 335 lines were obtained, consisting of 104 and 231 lines derived from Ar- and C-ion irradiation, respectively. The change in the DNA content of the lines was analyzed by flow cytometry to evaluate the irradiation-induced damage to the DNA. The two lines demonstrated significant differences in the DNA content and changes at the chromosome level. The screening for the morphological mutants was conducted in the field. Some irradiated lines showed inhibited or no tuberous root phenotype as mutant candidates. Additionally, the high-yield mutant candidates were dominated by Ar-ion irradiation. It was indicated that heavy-ion beam mutagenesis is effective in broadening the range of the phenotypes corresponding to tuberous root formation in hexaploid sweet potato.
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Claims 9
Claim · model-written summary
High doses of each ion inhibited shoot regeneration.
- Taxon
- sweet potato
_Flower.jpg?width=60)
Ipomoea batatast0 - Treatment
- each ion irradiatione0
- Outcome
- shoot regeneratione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“The shoot regeneration was inhibited at high doses of each ion irradiation.”
t0“sweet potato”
Claim · model-written summary
Ar ion irradiation had a strong effect on shoot regeneration.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- Ar-ion irradiatione0
- Outcome
- shoot regeneratione0
- Direction
- high biological effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ar-ion irradiation had an especially high biological effect on shoot regeneration.”
t0“sweet potato”
Claim · model-written summary
104 sweet potato lines were derived from Ar- irradiation.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- Ar-e0
- Outcome
- linese0
- Value
- 104e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 335 lines were obtained, consisting of 104 and 231 lines derived from Ar- and C-ion irradiation, respectively.”
t0“sweet potato”
Claim · model-written summary
231 sweet potato lines were derived from C-ion irradiation irradiation.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- C-ion irradiatione0
- Outcome
- linese0
- Value
- 231e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 335 lines were obtained, consisting of 104 and 231 lines derived from Ar- and C-ion irradiation, respectively.”
t0“sweet potato”
Claim · model-written summary
Two lines differed significantly in DNA content.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- two linese0
- Outcome
- DNA contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two lines demonstrated significant differences in the DNA content and changes at the chromosome level.”
t0“sweet potato”
Claim · model-written summary
Two lines showed chromosome level changes.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- two linese0
- Outcome
- changes at the chromosome levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two lines demonstrated significant differences in the DNA content and changes at the chromosome level.”
t0“sweet potato”
Claim · model-written summary
Some irradiated lines lacked or had inhibited tuberous root formation.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- irradiated linese0
- Outcome
- inhibited or no tuberous root phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some irradiated lines showed inhibited or no tuberous root phenotype as mutant candidates.”
t0“sweet potato”
Claim · model-written summary
Ar ion irradiation predominated among high yield mutant candidates.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- Ar-ion irradiatione0
- Outcome
- high-yield mutant candidatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the high-yield mutant candidates were dominated by Ar-ion irradiation.”
t0“sweet potato”
Claim · model-written summary
Heavy ion mutagenesis broadened tuberous root phenotypic variation.
- Taxon
- sweet potatoIpomoea batatase0
- Outcome
- range of the phenotypese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heavy-ion beam mutagenesis is effective in broadening the range of the phenotypes corresponding to tuberous root formation in hexaploid sweet potato.”
The same questions in other papers
radiation · growth · Ipomoea batatas (sweet potato)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.