Paper · 2017 · DOAJ (DOAJ: Directory of Open Access Journals)

Investigation of Nitrogen Fixation Efficiency of Some Azotobacter Isolates by Maize Inoculation

m Leylasi Marand; m Sarikhani

6
claims
12
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by-sa

Abstract

Nitrogen fixation ability in free-living condition is one of the properties of diazotrophic bacteria that Azotobacter is one of the famous genera of diazotrophs. Evaluation of nitrogen fixation efficiency can be measured by different methods, such as difference method and plant inoculation. Thus, N2 fixation ability of 18 bacterial isolates such as Azotobacter was measured using completely randomized design with three replications. Bacterial liquid culture was added to bagasse- perlite as a carrier and disinfected seeds were inoculated with prepared inoculants. Treatments were included, negative controls (including carrier without bacteria and without carrier), positive controls (using urea equal to 50% and 100% of fertilizer recommendation) and pots inoculated with bacterial inoculants. Results showed that expect wet weight and nitrogen concentration of root, all measured parameters were significantly influenced by treatments. Other measured parameters such as shoot wet and dry weight, root dry weight, total wet and dry weight, height, diameter and chlorophyll index, were significantly influenced by 50% and 100% fertilizer treatment and highest mean was recorded in these treatments, while among bacterial treatments A. chroococcum 14SP-Ι, A. chroococcum 16SP-2 and Pseudomonas sp. 34SP-Ш were effective. The highest total nitrogen content and nitrogen translocation factor were observed in plants which inoculated by A. chroococcum 16SP-2 that caused an increasing of 32% and 148% compared to negative control (without carrier and bacteria). Also, the highest nitrogen fixation efficiency was measured in 16SP-2 isolate equal to 39.47% by difference method.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation with A. chroococcum 16SP-2 increased maize total nitrogen content by 32% relative to the negative control

Taxon
MaizeZea mayst0
Treatment
inoculated by A. chroococcum 16SP-2e1
Interactor
A. chroococcum 16SP-2Azotobacter chroococcume1symbiont
Outcome
total nitrogen contente1
Direction
increasinge1
Value
32%e1
Comparator
negative control (without carrier and bacteria)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Maize Inoculation”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
e1

“The highest total nitrogen content and nitrogen translocation factor were observed in plants which inoculated by A. chroococcum 16SP-2 that caused an increasing of 32% and 148% compared to negative control (without carrier and bacteria)”

✓ located verbatim in the source · abstract, characters 1324–1560 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation with A. chroococcum 16SP-2 increased maize nitrogen translocation factor by 148% relative to the negative control

Taxon
MaizeZea mayst0
Treatment
inoculated by A. chroococcum 16SP-2e1
Interactor
A. chroococcum 16SP-2Azotobacter chroococcume1symbiont
Outcome
nitrogen translocation factore1
Direction
increasinge1
Value
148%e1
Comparator
negative control (without carrier and bacteria)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Maize Inoculation”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
e1

“The highest total nitrogen content and nitrogen translocation factor were observed in plants which inoculated by A. chroococcum 16SP-2 that caused an increasing of 32% and 148% compared to negative control (without carrier and bacteria)”

✓ located verbatim in the source · abstract, characters 1324–1560 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The 16SP-2 isolate had the highest nitrogen fixation efficiency, 39.47% by the difference method

Taxon
MaizeZea mayst0
Material
16SP-2 isolatee2
Interactor
16SP-2e2symbiont
Outcome
nitrogen fixation efficiencye2
Value
39.47%e2
Qualifier
by difference methode2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Maize Inoculation”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
e2

“the highest nitrogen fixation efficiency was measured in 16SP-2 isolate equal to 39.47% by difference method”

✓ located verbatim in the source · abstract, characters 1568–1676 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Among the bacterial treatments, the Azotobacter and Pseudomonas isolates named in the abstract were effective on maize

Taxon
MaizeZea mayst0
Outcome
effectivee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Maize Inoculation”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
e3

“were effective”

✓ located verbatim in the source · abstract, characters 1308–1322 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

50% and 100% fertilizer treatments significantly raised the measured growth parameters of maize, with highest means in these treatments

Taxon
MaizeZea mayst0
Treatment
50% and 100% fertilizer treatmente4
Outcome
Other measured parameterse4
Direction
highest meane4
Qualifier
shoot wet and dry weight, root dry weight, total wet and dry weight, height, diameter and chlorophyll indexe4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Maize Inoculation”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
e4

“Other measured parameters such as shoot wet and dry weight, root dry weight, total wet and dry weight, height, diameter and chlorophyll index, were significantly influenced by 50% and 100% fertilizer treatment and highest mean was recorded in these treatments”

✓ located verbatim in the source · abstract, characters 942–1201 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

All measured maize parameters were significantly influenced by treatments, except root wet weight and root nitrogen concentration

Taxon
MaizeZea mayst0
Outcome
all measured parameterse5
Direction
significantly influencede5
Qualifier
expect wet weight and nitrogen concentration of roote5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Maize Inoculation”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
e5

“Results showed that expect wet weight and nitrogen concentration of root, all measured parameters were significantly influenced by treatments”

✓ located verbatim in the source · abstract, characters 799–940 · show in text ↑

Azotobacter chroococcum · nutrient: content · Zea mays (maize)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

inoculation · nutrient: content · Zea mays (maize)Positive and negative results

306 papers: 288 positive, 18 negative or no effect

287 papers in the same direction as this paper · 16 in the opposite direction · 2 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

302 papers more in this group

See all 306 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.