Abstract
Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide, including potato late blight caused by P. infestans and soybean root rot caused by P. sojae. Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate (PI3P) to promote infection via direct association with RxLR effectors. Here, we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1 (AtPIP5K1), which can phosphorylate PI3P to PI(3,4)P2. We fused AtPIP5K1 with the soybean PR1a signal peptide (SP-PIP5K1) to enable its secretion into the plant apoplast. Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P. sojae and P. infestans isolates, respectively. SP-PIP5K1 significantly reduced PI3P accumulation during P. sojae and soybean interaction. Knockout or inhibition of PI3 kinases (PI3Ks) in P. sojae compromised the resistance mediated by SP-PIP5K1, indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P. Furthermore, we revealed that SP-PIP5K1 can interfere with the action of P. sojae mediated by the RxLR effector Avr1k. This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Transgenic soybean expressing SP-PIP5K1 showed substantially enhanced resistance to P. sojae isolates.
- Taxon
- soybean
Glycine maxe0 - Treatment
- expressing SP-PIP5K1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- SP-PIP5K1e0
- Outcome
- resistance to various P. sojaee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P. sojae and P. infestans isolates, respectively”
Claim · model-written summary
Transgenic potato expressing SP-PIP5K1 showed substantially enhanced resistance to P. infestans isolates.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- expressing SP-PIP5K1e0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- SP-PIP5K1e0
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P. sojae and P. infestans isolates, respectively”
Claim · model-written summary
SP-PIP5K1 significantly reduced PI3P accumulation during the P. sojae–soybean interaction.
- Taxon
- soybeanGlycine maxe0
- Treatment
- SP-PIP5K1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- SP-PIP5K1e0
- Outcome
- PI3P accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SP-PIP5K1 significantly reduced PI3P accumulation during P. sojae and soybean interaction”
Claim · model-written summary
Knocking out or inhibiting P. sojae PI3Ks compromised SP-PIP5K1-mediated resistance.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Knockout or inhibition of PI3 kinases (PI3Ks) in P. sojaee0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- PI3 kinases (PI3Ks)e0
- Outcome
- resistance mediated by SP-PIP5K1e0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout or inhibition of PI3 kinases (PI3Ks) in P. sojae compromised the resistance mediated by SP-PIP5K1”
t0“P. sojae and soybean interaction”
Claim · model-written summary
SP-PIP5K1 interfered with the action of P. sojae mediated by Avr1k.
- Taxon
- soybeanGlycine maxt0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- SP-PIP5K1e0
- Outcome
- action of P. sojae mediated by the RxLR effector Avr1ke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SP-PIP5K1 can interfere with the action of P. sojae mediated by the RxLR effector Avr1k”
t0“P. sojae and soybean interaction”
The same questions in other papers
Phytophthora sojae · disease · Glycine max (soybean)Positive and negative results
1 in the opposite direction
- positive
loss of function · disease · Glycine max (soybean)Positive and negative results
19 papers in the same direction as this paper · 17 in the opposite direction
- positive
- positive
- positive
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.