Abstract
Powdery mildew caused by Blumeria graminis f. sp. tritici ( Bgt ) is a destructive disease of wheat throughout the world. Host resistance is considered the most sustainable way to control this disease. Powdery mildew resistance gene Pm2b was mapped to the same genetic interval with Pm2a and PmCH1357 cloned previously, but showed different resistance spectra from them, indicating that they might be caused by different resistance genes or alleles. In this study, Pm2b was delimited to a 1.64 Mb physical interval using a large segregating population containing 4,354 F 2:3 families of resistant parent KM2939 and susceptible cultivar Shimai 15. In this interval, TraesCS5D03G0111700 encoding the coiled-coil nucleotide-binding site leucine-rich repeat protein (CC-NBS-LRR) was determined as the candidate gene of Pm2b . Silencing by barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) technology and two independent mutants analysis in KM2939 confirmed the candidate gene TraesCS5D03G0111700 was Pm2b . The sequence of Pm2b was consistent with Pm2a/PmCH1357. Subcellular localization showed Pm2b was located on the cell nucleus and plasma membrane. Pm2b had the highest expression level in leaves and was rapidly up-regulated after inoculating with Bgt isolate E09. The yeast two-hybrid (Y2H) and luciferase complementation imaging assays (LCI) showed that PM2b could self-associate through the NB domain. Notably, we identified PM2b interacting with the transcription factor TaWRKY76-D, which depended on the NB domain of PM2b and WRKY domain of TaWRKY76-D. TaWRKY76-D negatively regulated the resistance to powdery mildew in wheat. The specific KASP marker K529 could take the advantage of high-throughput and high-efficiency for detecting Pm2b and be useful in molecular marker assisted-selection breeding. In conclusion, cloning and disease resistance mechanism analysis of Pm2b provided an example to emphasize a need of the molecular isolation of resistance genes, which has implications in marker assisted wheat breeding.
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Claims 11
Claim · model-written summary
Pm2b was delimited to a 1.64 Mb physical interval.
- Taxon
- common wheat
Triticum aestivumt0 - Gene
- Pm2be0
- Outcome
- physical intervale0
- Value
- 1.64 Mbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pm2b was delimited to a 1.64 Mb physical interval”
t0“common wheat”
Claim · model-written summary
TraesCS5D03G0111700 was identified as the candidate gene of Pm2b.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TraesCS5D03G0111700e0
- Outcome
- candidate gene of Pm2be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TraesCS5D03G0111700 encoding the coiled-coil nucleotide-binding site leucine-rich repeat protein (CC-NBS-LRR) was determined as the candidate gene of Pm2b”
t0“common wheat”
Claim · model-written summary
Silencing and mutant analyses confirmed TraesCS5D03G0111700 as Pm2b.
- Taxon
- common wheatTriticum aestivumt0
- Material
- KM2939e0
- Gene
- TraesCS5D03G0111700e0
- Outcome
- confirmed the candidate gene TraesCS5D03G0111700 was Pm2be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing by barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) technology and two independent mutants analysis in KM2939 confirmed the candidate gene TraesCS5D03G0111700 was Pm2b”
t0“common wheat”
Claim · model-written summary
Pm2b sequence was consistent with Pm2a/PmCH1357.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- Pm2be0
- Outcome
- sequence of Pm2b was consistent with Pm2a/PmCH1357e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequence of Pm2b was consistent with Pm2a/PmCH1357”
t0“common wheat”
Claim · model-written summary
Pm2b localized to the nucleus and plasma membrane.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- Pm2be0
- Outcome
- located on the cell nucleus and plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pm2b was located on the cell nucleus and plasma membrane”
t0“common wheat”
Claim · model-written summary
Pm2b expression was highest in leaves.
- Taxon
- common wheatTriticum aestivumt0
- Organ / stage
- leavese0
- Gene
- Pm2be0
- Outcome
- expression levele0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pm2b had the highest expression level in leaves”
t0“common wheat”
Claim · model-written summary
Pm2b expression rose rapidly after Bgt isolate E09 inoculation.
- Taxon
- common wheatTriticum aestivumt0
- Treatment
- inoculating with Bgt isolate E09e0
- Interactor
- Bgt isolate E09e0pathogen
- Gene
- Pm2be1
- Outcome
- expression levele1
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was rapidly up-regulated after inoculating with Bgt isolate E09”
e1“Pm2b had the highest expression level in leaves”
t0“common wheat”
Claim · model-written summary
PM2b self-associated through its NB domain.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- PM2be0
- Outcome
- self-associate through the NB domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PM2b could self-associate through the NB domain”
t0“common wheat”
Claim · model-written summary
PM2b interacted with TaWRKY76-D through their NB and WRKY domains.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaWRKY76-De0
- Outcome
- PM2b interacting with the transcription factor TaWRKY76-De0
- Qualifier
- depended on the NB domain of PM2b and WRKY domain of TaWRKY76-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PM2b interacting with the transcription factor TaWRKY76-D, which depended on the NB domain of PM2b and WRKY domain of TaWRKY76-D”
t0“common wheat”
Claim · model-written summary
TaWRKY76-D negatively regulated wheat powdery mildew resistance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaWRKY76-De0
- Outcome
- resistance to powdery mildewe0
- Direction
- negatively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY76-D negatively regulated the resistance to powdery mildew in wheat”
Claim · model-written summary
KASP marker K529 was presented as a useful high-throughput marker for Pm2b-assisted selection.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- Pm2be0
- Outcome
- detecting Pm2be0
- Qualifier
- high-throughput and high-efficiencye0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The specific KASP marker K529 could take the advantage of high-throughput and high-efficiency for detecting Pm2b and be useful in molecular marker assisted-selection breeding”
t0“common wheat”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
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