Abstract
Double-stranded RNA-binding proteins are small molecules in the RNA interference (RNAi) pathway that form the RNAi machinery together with the Dicer-like protein (DCL) as a cofactor. This machinery cuts double-stranded RNA (dsRNA) to form multiple small interfering RNAs (siRNAs). Our goal was to clarify the function of DRB in tomato resistant to TYLCV. In this experiment, the expression of the SlDRB1 and SlDRB4 genes was analyzed in tomato leaves by qPCR, and the function of SlDRB1 and SlDRB4 in resistance to TYLCV was investigated by virus-induced gene silencing (VIGS). Then, peroxidase activity was determined. The results showed that the expression of SlDRB1 gradually increased after inoculation of ‘dwarf tomato’ plants with tomato yellow leaf curl virus (TYLCV), but this gene was suppressed after 28 days. Resistance to TYLCV was significantly weakened after silencing of the SlDRB1 gene. However, there were no significant expression differences in SlDRB4 after TYLCV inoculation. Our study showed that silencing SlDRB1 attenuated the ability of tomato plants to resist virus infection; therefore, SlDRB1 may play a key role in the defense against TYLCV in tomato plants, whereas SlDRB4 is likely not involved in this defense response. Taken together, These results suggest that the DRB gene is involved in the mechanism of antiviral activity.
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Claims 5
Claim · model-written summary
SlDRB1 expression rose after TYLCV inoculation of dwarf tomato, then was suppressed after 28 days.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- dwarf tomatoe0
- Treatment
- inoculation of ‘dwarf tomato’ plants with tomato yellow leaf curl virus (TYLCV)e0
- Interactor
- tomato yellow leaf curl virus (TYLCV)Tomato yellow leaf curl viruse0pathogen
- Gene
- SlDRB1e0
- Outcome
- expression of SlDRB1e0
- Direction
- gradually increasede0
- Qualifier
- suppressed after 28 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of SlDRB1 gradually increased after inoculation of ‘dwarf tomato’ plants with tomato yellow leaf curl virus (TYLCV), but this gene was suppressed after 28 days.”
Claim · model-written summary
Silencing SlDRB1 significantly weakened tomato resistance to TYLCV.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- silencing of the SlDRB1 genee0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- SlDRB1e0
- Outcome
- Resistance to TYLCVe0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Resistance to TYLCV was significantly weakened after silencing of the SlDRB1 gene.”
t0“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlDRB4 expression did not differ significantly after TYLCV inoculation.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- TYLCV inoculatione0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- SlDRB4e0
- Outcome
- expression differences in SlDRB4e0
- Direction
- no significant expression differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were no significant expression differences in SlDRB4 after TYLCV inoculation.”
t0“tomato (Solanum lycopersicum)”
Claim · model-written summary
The authors infer that SlDRB1 may play a key role in tomato defense against TYLCV.
- Taxon
- tomato plantsSolanum lycopersicume0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- SlDRB1e0
- Outcome
- defense against TYLCVe0
- Qualifier
- may play a key rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlDRB1 may play a key role in the defense against TYLCV in tomato plants”
Claim · model-written summary
The authors infer that SlDRB4 is likely not involved in tomato defense against TYLCV.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- tomato yellow leaf curl virus (TYLCV)Tomato yellow leaf curl virust0pathogen
- Gene
- SlDRB4e0
- Outcome
- defense responsee0
- Qualifier
- likely not involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlDRB4 is likely not involved in this defense response.”
t0“tomato yellow leaf curl virus (TYLCV) in tomato (Solanum lycopersicum)”
The same questions in other papers
loss of function · Tomato yellow leaf curl virus · Solanum lycopersicum (tomato)Positive and negative results
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- positive
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4 papers in the same direction as this paper · 10 in the opposite direction
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.