Abstract
CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.
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Claims 4
Claim · model-written summary
Engineered plants had low TYLCV DNA accumulation following genome targeting.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- Targeting the TYLCV genome with Cas9-single guide RNAe0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Outcome
- TYLCV DNA genomee0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants.”
e1“Nicotiana benthamiana and tomato (Solanum lycopersicum) plants”
Claim · model-written summary
Engineered tomato plants had low TYLCV DNA accumulation following genome targeting.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- Targeting the TYLCV genome with Cas9-single guide RNAe0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Outcome
- TYLCV DNA genomee0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants.”
e1“Nicotiana benthamiana and tomato (Solanum lycopersicum) plants”
Claim · model-written summary
CRISPR/Cas9 immunity persisted across multiple generations of Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Outcome
- CRISPR/Cas9-based immunitye0
- Qualifier
- across multiple generationse0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants.”
Claim · model-written summary
CRISPR/Cas9 immunity persisted across multiple generations of tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- CRISPR/Cas9-based immunitye0
- Qualifier
- across multiple generationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants.”
The same questions in other papers
Solanum lycopersicum · Tomato yellow leaf curl virus · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- negative
3 papers more in this group
See all 7 papers →loss of function · Tomato yellow leaf curl virus · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- negative
2 papers more in this group
See all 6 papers →loss of function · Tomato yellow leaf curl virus · Solanum lycopersicum (tomato)Positive and negative results
3 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.