Abstract
Gamma-ray has been used to increase genetic variation to obtain salt-tolerant plants in strawberries.The protocol was established to multiply strawberry cv. Albion from runner segments cultured on multiplication Murashige and Skoog (MS) medium contain 0.5 mg l-1 of 6-benzyl adenine (BA) and 0.1 mg l-1 of Naphthaleneacetic acid (NAA). Cultures were irradiated with gamma rays at (0, 20, 50, 100) Gy after 30 days, and the irradiated and unirradiated shoots were exposed to different concentrations of Sodium Chloride (NaCl) (6,10,14, 22) dS m-1. The results showed the superiority of doses 20 and 50 Gy in giving the highest rate of the number of shoots reached (9.25 and 8.44) shoot explant-1. The treatment 6 dS m-1 NaCl with 20 Gy was superior in giving the highest fresh 4.75 g and dry weight 0.36 g. A significant increase of proline was observed in the shoots irradiated with a dose of 50 Gy and grown on a medium with 22 mg l-1 of NaCl, as it reached 34.36 (µm proline g-1 fresh weight) compared 6 dS m-1 and unirradiated media and the highest enzyme activity of (POD) was )263.50 units g-1 FW ( when treated with 100 Gy grown on a medium with 22 ds m-1 of salt. While the dose exceeded 20 Gy without adding salt, as it gave the highest activity of (CAT) enzyme, reaching )4.042 units g-1 FW(. It was observed that multiplication was generally restricted, depending on the increase in salt applications and gamma rays. Keywords: BA, NAA, Fragaria, Micropropagation, mutation gamma ray. Salt tolerance.
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Claims 7
Claim · model-written summary
Gamma irradiation at 20 and 50 Gy gave the highest number of strawberry shoots per explant.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Material
- cv. Albione0
- Treatment
- doses 20 and 50 Gye1
- Outcome
- number of shootse1
- Direction
- higheste1
- Value
- 9.25 and 8.44e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e1“The results showed the superiority of doses 20 and 50 Gy in giving the highest rate of the number of shoots reached (9.25 and 8.44) shoot explant-1”
Claim · model-written summary
Irradiation at 20 Gy combined with 6 dS m-1 NaCl gave the highest strawberry fresh weight, 4.75 g.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- 6 dS m-1 NaCl with 20 Gye1
- Outcome
- freshe1
- Direction
- higheste1
- Value
- 4.75 ge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e1“The treatment 6 dS m-1 NaCl with 20 Gy was superior in giving the highest fresh 4.75 g and dry weight 0.36 g”
Claim · model-written summary
Irradiation at 20 Gy combined with 6 dS m-1 NaCl gave the highest strawberry dry weight, 0.36 g.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- 6 dS m-1 NaCl with 20 Gye1
- Outcome
- dry weighte1
- Direction
- higheste1
- Value
- 0.36 ge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e1“The treatment 6 dS m-1 NaCl with 20 Gy was superior in giving the highest fresh 4.75 g and dry weight 0.36 g”
Claim · model-written summary
Shoots irradiated at 50 Gy and grown on 22 mg l-1 NaCl showed a significant increase in proline, reaching 34.36 compared with 6 dS m-1 and unirradiated media.
- Taxon
- strawberryFragaria ×ananassae0
- Organ / stage
- shootse2
- Treatment
- dose of 50 Gye2
- Outcome
- prolinee2
- Direction
- increasee2
- Value
- 34.36e2
- Comparator
- unirradiated mediae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e2“A significant increase of proline was observed in the shoots irradiated with a dose of 50 Gy and grown on a medium with 22 mg l-1 of NaCl, as it reached 34.36”
e3“compared 6 dS m-1 and unirradiated media”
Claim · model-written summary
Shoots irradiated at 100 Gy and grown on 22 ds m-1 salt had the highest peroxidase (POD) enzyme activity, 263.50 units g-1 FW.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- 100 Gye4
- Gene
- PODe4
- Outcome
- enzyme activitye4
- Direction
- higheste4
- Value
- 263.50e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e4“the highest enzyme activity of (POD) was )263.50 units g-1 FW ( when treated with 100 Gy grown on a medium with 22 ds m-1 of salt”
Claim · model-written summary
Irradiation above 20 Gy without salt gave the highest catalase (CAT) enzyme activity, 4.042 units g-1 FW.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- dose exceeded 20 Gye5
- Gene
- CATe5
- Outcome
- activity of (CAT) enzymee5
- Direction
- higheste5
- Value
- 4.042e5
- Qualifier
- without adding salte5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e5“While the dose exceeded 20 Gy without adding salt, as it gave the highest activity of (CAT) enzyme, reaching )4.042 units g-1 FW”
Claim · model-written summary
Multiplication of strawberry was generally restricted as salt applications and gamma rays increased.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- salt applications and gamma rayse5
- Outcome
- multiplicatione5
- Direction
- restrictede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol was established to multiply strawberry cv. Albion”
e5“It was observed that multiplication was generally restricted, depending on the increase in salt applications and gamma rays”
The same questions in other papers
sodium chloride · growth · Fragaria x ananassa (strawberry)Results differ
2 papers in the same direction as this paper · 14 in the opposite direction
- negative
- negative
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.