Abstract
The genes encoding the phosphate uptake system in Xanthomonas citri pv. glycines 12-2 were previously found to be upregulated when in soybean leaves. This study thus explored the role of the phosphate uptake system on its virulence to soybean. While phoB and pstSCAB mutants were greatly impaired in both inciting disease symptoms and growth in soybean, the virulence and growth in soybean of a phoU mutant was not reduced when compared with the wild-type strain. The expression of phoB and pstSCAB was highly induced in phosphate-deficient media. In addition, the expression of phoB, assessed with a fusion to a promoterless ice nucleation reporter gene, was greatly increased in soybean leaves, confirming that the soybean apoplast is a phosphorus-limited habitat for X. citri pv. glycines. Global gene expression profiles of phoB and phoU mutants of X. citri pv. glycines conducted under phosphate-limitation conditions in vitro, using RNA-seq, revealed that PhoB positively regulated genes involved in signal transduction, the xcs cluster type II secretion system, cell motility, and chemotaxis, while negatively regulating cell wall and membrane biogenesis, DNA replication and recombination and repair, and several genes with unknown function. PhoU also positively regulated the same genes involved in cell motility and chemotaxis. The severity of bacterial pustule disease was decreased in soybean plants grown under high phosphate fertilization conditions, demonstrating that high phosphate availability in soybean plants can affect infection by X. citri pv. glycines by modulation of the expression of phosphate uptake systems. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
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Claims 10
Claim · model-written summary
phoB and pstSCAB mutants of X. citri pv. glycines were greatly impaired in disease symptoms and growth in soybean.
- Taxon
- soybean
Glycine maxe1 - Treatment
- phoB and pstSCAB mutantse1
- Interactor
- Xanthomonas citri pv. glycinesXanthomonas citri pv. glycinese0pathogen
- Outcome
- disease symptoms and growthe1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“While phoB and pstSCAB mutants were greatly impaired in both inciting disease symptoms and growth in soybean”
e0“genes encoding the phosphate uptake system in Xanthomonas citri pv. glycines 12-2”
Claim · model-written summary
The phoU mutant of X. citri pv. glycines did not have reduced virulence or growth in soybean compared with the wild-type strain.
- Taxon
- soybeanGlycine maxe2
- Treatment
- phoU mutante2
- Interactor
- Xanthomonas citri pv. glycinesXanthomonas citri pv. glycinese0pathogen
- Outcome
- virulence and growthe2
- Direction
- not reducede2
- Comparator
- wild-type straine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“the virulence and growth in soybean of a phoU mutant was not reduced when compared with the wild-type strain”
e0“genes encoding the phosphate uptake system in Xanthomonas citri pv. glycines 12-2”
Claim · model-written summary
Expression of phoB and pstSCAB was highly induced in phosphate-deficient media.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- phosphate-deficient mediae3
- Gene
- phoB and pstSCABe3
- Outcome
- expressione3
- Direction
- highly inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The expression of phoB and pstSCAB was highly induced in phosphate-deficient media”
t0“Virulence in Soybean”
Claim · model-written summary
phoB expression was greatly increased in soybean leaves.
- Taxon
- soybeanGlycine maxe4
- Organ / stage
- soybean leavese4
- Gene
- phoBe4
- Outcome
- expressione4
- Direction
- greatly increasede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“the expression of phoB, assessed with a fusion to a promoterless ice nucleation reporter gene, was greatly increased in soybean leaves”
Claim · model-written summary
The soybean apoplast is a phosphorus-limited habitat for X. citri pv. glycines.
- Taxon
- soybeanGlycine maxe5
- Interactor
- X. citri pv. glycinesXanthomonas citri pv. glycinese5pathogen
- Outcome
- phosphorus-limited habitate5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“confirming that the soybean apoplast is a phosphorus-limited habitat for X. citri pv. glycines”
Claim · model-written summary
PhoB positively regulated genes involved in signal transduction and the xcs cluster type II secretion system in X. citri pv. glycines under phosphate limitation in vitro.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- phoB and phoU mutantse6
- Interactor
- X. citri pv. glycinesXanthomonas citri pv. glycinese6pathogen
- Gene
- PhoBe6
- Outcome
- signal transduction, the xcs cluster type II secretion systeme6
- Direction
- positively regulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Global gene expression profiles of phoB and phoU mutants of X. citri pv. glycines conducted under phosphate-limitation conditions in vitro, using RNA-seq, revealed that PhoB positively regulated genes involved in signal transduction, the xcs cluster type II secretion system, cell motility, and chemotaxis, while negatively regulating cell wall and membrane biogenesis, DNA replication and recombination and repair, and several genes with unknown function”
t0“Virulence in Soybean”
Claim · model-written summary
PhoB positively regulated genes involved in cell motility and chemotaxis in X. citri pv. glycines under phosphate limitation in vitro.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- phoB and phoU mutantse6
- Interactor
- X. citri pv. glycinesXanthomonas citri pv. glycinese6pathogen
- Gene
- PhoBe6
- Outcome
- cell motility, and chemotaxise6
- Direction
- positively regulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Global gene expression profiles of phoB and phoU mutants of X. citri pv. glycines conducted under phosphate-limitation conditions in vitro, using RNA-seq, revealed that PhoB positively regulated genes involved in signal transduction, the xcs cluster type II secretion system, cell motility, and chemotaxis, while negatively regulating cell wall and membrane biogenesis, DNA replication and recombination and repair, and several genes with unknown function”
t0“Virulence in Soybean”
Claim · model-written summary
PhoB negatively regulated cell wall and membrane biogenesis, DNA replication and recombination and repair, and several genes with unknown function in X. citri pv. glycines.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- phoB and phoU mutantse6
- Interactor
- X. citri pv. glycinesXanthomonas citri pv. glycinese6pathogen
- Gene
- PhoBe6
- Outcome
- cell wall and membrane biogenesis, DNA replication and recombination and repair, and several genes with unknown functione6
- Direction
- negatively regulatinge6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Global gene expression profiles of phoB and phoU mutants of X. citri pv. glycines conducted under phosphate-limitation conditions in vitro, using RNA-seq, revealed that PhoB positively regulated genes involved in signal transduction, the xcs cluster type II secretion system, cell motility, and chemotaxis, while negatively regulating cell wall and membrane biogenesis, DNA replication and recombination and repair, and several genes with unknown function”
t0“Virulence in Soybean”
Claim · model-written summary
PhoU positively regulated the same genes involved in cell motility and chemotaxis in X. citri pv. glycines.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- phoU mutantse1
- Interactor
- X. citri pv. glycinesXanthomonas citri pv. glycinese1pathogen
- Gene
- PhoUe7
- Outcome
- genes involved in cell motility and chemotaxise7
- Direction
- positively regulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“PhoU also positively regulated the same genes involved in cell motility and chemotaxis”
e1“phoB and phoU mutants of X. citri pv. glycines”
t0“Virulence in Soybean”
Claim · model-written summary
High phosphate fertilization decreased the severity of bacterial pustule disease in soybean.
- Taxon
- soybeanGlycine maxe1
- Treatment
- high phosphate fertilization conditionse1
- Interactor
- X. citri pv. glycinesXanthomonas citri pv. glycinese2pathogen
- Outcome
- severity of bacterial pustule diseasee1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The severity of bacterial pustule disease was decreased in soybean plants grown under high phosphate fertilization conditions”
e2“high phosphate availability in soybean plants can affect infection by X. citri pv. glycines”