Abstract
Abstract In vitro -derived shoots of ‘Improved Blaze’ rose ( Rosa hybrida L.) were used to investigate the cause of improved root initiation obtained by lowering the concentration of the Murashige and Skoog (MS) salt formulation in the nutrient medium. The number and length of roots per explant increased as the concentration of total nitrogen in the MS salt formulation was reduced from 60 to 7.5 m m . There was no effect on rooting with as much as a 16-fold reduction in the concentration of the remaining MS salts from that of the normal MS formulation, when total nitrogen was kept at a constant 7.5 m m . When nitrogen was maintained at 7.5 m m and the concentrations of the remaining salts were maintained at 1/2 times that of the MS salt formulation, rooting was unaffected by raising the total solute concentration with NaCl to that when the medium contained the full MS nitrogen salt complement. These results show that lowering the total mineral salt level in the nutrient medium provides a more favorable nitrogen salts concentration for rhizogenesis than that provided by the MS salt formulation.
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Claims 3
Claim · model-written summary
Lowering total nitrogen in the MS salt formulation from 60 to 7.5 mM increased the number and length of roots per rose explant.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare1 - Material
- Improved Blazee1
- Organ / stage
- shootse1
- Treatment
- total nitrogen in the MS salt formulation was reduced from 60 to 7.5 m me0
- Outcome
- number and length of roots per explante0
- Direction
- increasede0
- Setting
- In vitroe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number and length of roots per explant increased as the concentration of total nitrogen in the MS salt formulation was reduced from 60 to 7.5 m m”
e1“In vitro -derived shoots of ‘Improved Blaze’ rose ( Rosa hybrida L.)”
Claim · model-written summary
Lowering the other MS salts 16-fold had no effect on rooting while nitrogen was held at 7.5 mM.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Material
- Improved Blazee1
- Treatment
- 16-fold reduction in the concentration of the remaining MS saltse0
- Outcome
- rootinge0
- Direction
- no effecte0
- Qualifier
- total nitrogen was kept at a constant 7.5 m me0
- Comparator
- normal MS formulatione0
- Setting
- In vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no effect on rooting with as much as a 16-fold reduction in the concentration of the remaining MS salts from that of the normal MS formulation, when total nitrogen was kept at a constant 7.5 m m”
e1“In vitro -derived shoots of ‘Improved Blaze’ rose ( Rosa hybrida L.)”
Claim · model-written summary
Raising total solute concentration with NaCl to the level of full MS nitrogen salts left rooting unaffected.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Material
- Improved Blazee1
- Treatment
- raising the total solute concentration with NaCle0
- Outcome
- rootinge0
- Direction
- unaffectede0
- Comparator
- full MS nitrogen salt complemente0
- Setting
- In vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting was unaffected by raising the total solute concentration with NaCl to that when the medium contained the full MS nitrogen salt complement”
e1“In vitro -derived shoots of ‘Improved Blaze’ rose ( Rosa hybrida L.)”
The same questions in other papers
salinity · growth · Rosa hybrid cultivarPositive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.