Abstract
In genome analyses of Rhizoctonia solani AG1-IA causing sheath blight (ShB) of rice, many genes were identified to have a hypothetical role in pathogenesis. To understand their roles in pathogenesis, their expressions during fungal infection were studied. An aggressive R. solani strain, RIRS-K, was first identified among six isolates, RIRS-K, RIRS-17, RIRS-S, RIRS-T, RIRS-MU and RIRS-FD, for inducing a maximum relative lesion height (RLH) of 32.7% on a ShB susceptible cultivar, Pusa Basmati-1. Hypothetical pathogenicity genes (52 nos) identified by in silico analyses of the publicly available genomic database of the pathogen strain were evaluated in Pathogen-Host Interaction (PHI) blast and RIRS-K. Though PHI blast identified 26 genes as potential ones, only 8 were constitutively expressive in RIRS-K cultured in a minimal broth. Among them, only expressions of AG1IA_06195, AG02692, AG04508, and AG05730 were induced in the rice plant inoculated with RIRS-K and, hence, were identified as the candidate ones. The candidate genes were highly expressed in the aggressive strain (RIRS-K) in comparison to the less aggressive one (RIRS-17). In further testing of their expressions in the highly aggressive fungal strain, RIRS-K infecting PB-1 pre-colonized by a potent biocontrol consortium comprising of Bacillus subtilis (S17TH), Pseudomonas putida (TEPF-Sungal-1), and Trichoderma harzianum (S17TH), the disease scoring and gene expression studies indicated that the candidate genes were downregulated. The studies, therefore, speculated that these genes might play a role in pathogen aggressiveness and ShB development.
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Claims 6
Claim · model-written summary
RIRS-K induced the maximum relative lesion height of 32.7% on Pusa Basmati-1.
- Taxon
- rice
Oryza sativat0 - Material
- Pusa Basmati-1e0
- Interactor
- R. solani
Rhizoctonia solanie0pathogen - Outcome
- relative lesion height (RLH)e0
- Direction
- maximume0
- Value
- 32.7%e0
- Qualifier
- RIRS-Ke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice (Oryza sativa L.)”
e0“An aggressive R. solani strain, RIRS-K, was first identified among six isolates, RIRS-K, RIRS-17, RIRS-S, RIRS-T, RIRS-MU and RIRS-FD, for inducing a maximum relative lesion height (RLH) of 32.7% on a ShB susceptible cultivar, Pusa Basmati-1.”
Claim · model-written summary
PHI blast identified 26 potential pathogenicity genes.
- Taxon
- riceOryza sativat0
- Interactor
- RIRS-Ke0pathogen
- Outcome
- genes as potential onese0
- Value
- 26 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice (Oryza sativa L.)”
e0“Though PHI blast identified 26 genes as potential ones, only 8 were constitutively expressive in RIRS-K cultured in a minimal broth.”
Claim · model-written summary
Eight potential genes were constitutively expressed in RIRS-K in minimal broth.
- Taxon
- riceOryza sativat0
- Interactor
- RIRS-Ke0pathogen
- Outcome
- constitutively expressivee0
- Value
- 8e0
- Setting
- minimal brothe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice (Oryza sativa L.)”
e0“only 8 were constitutively expressive in RIRS-K cultured in a minimal broth.”
Claim · model-written summary
Four candidate genes were induced in rice inoculated with RIRS-K.
- Taxon
- riceOryza sativae0
- Interactor
- RIRS-Ke0pathogen
- Gene
- AG1IA_06195, AG02692, AG04508, and AG05730e0
- Outcome
- expressionse0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only expressions of AG1IA_06195, AG02692, AG04508, and AG05730 were induced in the rice plant inoculated with RIRS-K”
Claim · model-written summary
Candidate genes were more highly expressed in RIRS-K than RIRS-17.
- Taxon
- riceOryza sativat0
- Interactor
- RIRS-Ke0pathogen
- Outcome
- candidate genese0
- Direction
- highly expressede0
- Comparator
- RIRS-17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice (Oryza sativa L.)”
e0“The candidate genes were highly expressed in the aggressive strain (RIRS-K) in comparison to the less aggressive one (RIRS-17).”
Claim · model-written summary
Candidate genes were downregulated during infection of biocontrol-precolonized PB-1.
- Taxon
- riceOryza sativat0
- Material
- PB-1e0
- Treatment
- pre-colonized by a potent biocontrol consortiume0
- Interactor
- RIRS-Ke0pathogen
- Second interactor
- Bacillus subtilis (S17TH), Pseudomonas putida (TEPF-Sungal-1), and Trichoderma harzianum (S17TH)
Pseudomonas putida
Bacillus subtilis
Trichoderma harzianume0biocontrol_agent - Outcome
- candidate genese0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice (Oryza sativa L.)”
e0“RIRS-K infecting PB-1 pre-colonized by a potent biocontrol consortium comprising of Bacillus subtilis (S17TH), Pseudomonas putida (TEPF-Sungal-1), and Trichoderma harzianum (S17TH), the disease scoring and gene expression studies indicated that the candidate genes were downregulated.”