Abstract
Verticillium wilt caused by Verticillium dahliae is a serious vascular disease in cotton (Gossypium spp.). V. dahliae induces the expression of the CAROTENOID CLEAVAGE DIOXYGENASE 7 (GauCCD7) gene involved in strigolactone (SL) biosynthesis in Gossypium australe, suggesting a role for SLs in Verticillium wilt resistance. We found that the SL analog rac-GR24 enhanced while the SL biosynthesis inhibitor TIS108 decreased cotton resistance to Verticillium wilt. Knock-down of GbCCD7 and GbCCD8b genes in island cotton (Gossypium barbadense) decreased resistance, whereas overexpression of GbCCD8b in upland cotton (Gossypium hirsutum) increased resistance to Verticillium wilt. Additionally, Arabidopsis (Arabidopsis thaliana) SL mutants defective in CCD7 and CCD8 putative orthologs were susceptible, whereas both Arabidopsis GbCCD7- and GbCCD8b-overexpressing plants were more resistant to Verticillium wilt than wild-type (WT) plants. Transcriptome analyses showed that several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathways, such as MYELOCYTOMATOSIS 2 (GbMYC2) and ABA-INSENSITIVE 5, respectively, were upregulated in the roots of WT cotton plants in responses to rac-GR24 and V. dahliae infection but downregulated in the roots of both GbCCD7- and GbCCD8b-silenced cotton plants. Furthermore, GbMYC2 suppressed the expression of GbCCD7 and GbCCD8b by binding to their promoters, which might regulate the homeostasis of SLs in cotton through a negative feedback loop. We also found that GbCCD7- and GbCCD8b-silenced cotton plants were impaired in V. dahliae-induced reactive oxygen species (ROS) accumulation. Taken together, our results suggest that SLs positively regulate cotton resistance to Verticillium wilt through crosstalk with the JA- and ABA-signaling pathways and by inducing ROS accumulation.
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Claims 15
Claim · model-written summary
V. dahliae induced GauCCD7 expression in Gossypium australe.
- Taxon
- Gossypium australe
Gossypium australee0 - Treatment
- V. dahliaee0
- Interactor
- V. dahliae
Verticillium dahliaee0pathogen - Gene
- GauCCD7e0
- Outcome
- expression of the CAROTENOID CLEAVAGE DIOXYGENASE 7 (GauCCD7) genee0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. dahliae induces the expression of the CAROTENOID CLEAVAGE DIOXYGENASE 7 (GauCCD7) gene involved in strigolactone (SL) biosynthesis in Gossypium australe”
Claim · model-written summary
rac-GR24 enhanced cotton resistance to Verticillium wilt.
- Taxon
- cotton
Gossypium hirsutume0 - Treatment
- rac-GR24e0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Outcome
- cotton resistance to Verticillium wilte0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SL analog rac-GR24 enhanced while the SL biosynthesis inhibitor TIS108 decreased cotton resistance to Verticillium wilt”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
TIS108 decreased cotton resistance to Verticillium wilt.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- TIS108e0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Outcome
- cotton resistance to Verticillium wilte0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SL analog rac-GR24 enhanced while the SL biosynthesis inhibitor TIS108 decreased cotton resistance to Verticillium wilt”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
GbCCD7 knockdown decreased island cotton wilt resistance.
- Taxon
- island cottone0
- Treatment
- Knock-down of GbCCD7e0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- GbCCD7e0
- Outcome
- resistancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knock-down of GbCCD7 and GbCCD8b genes in island cotton (Gossypium barbadense) decreased resistance”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
GbCCD8b knockdown decreased island cotton wilt resistance.
- Taxon
- island cottone0
- Treatment
- Knock-down of GbCCD7 and GbCCD8b genese0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- GbCCD8be0
- Outcome
- resistancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knock-down of GbCCD7 and GbCCD8b genes in island cotton (Gossypium barbadense) decreased resistance”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
GbCCD8b overexpression increased upland cotton wilt resistance.
- Taxon
- upland cottone0
- Treatment
- overexpression of GbCCD8be0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- GbCCD8be0
- Outcome
- resistance to Verticillium wilte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GbCCD8b in upland cotton (Gossypium hirsutum) increased resistance to Verticillium wilt”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
Arabidopsis SL mutants defective in CCD7 and CCD8 orthologs were susceptible.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- SL mutantse0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- CCD7 and CCD8e0
- Outcome
- were susceptiblee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana) SL mutants defective in CCD7 and CCD8 putative orthologs were susceptible”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
Arabidopsis GbCCD7 overexpressors were more resistant than wild type.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- GbCCD7-e0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- GbCCD7e0
- Outcome
- resistant to Verticillium wilte0
- Direction
- more resistante0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both Arabidopsis GbCCD7- and GbCCD8b-overexpressing plants were more resistant to Verticillium wilt than wild-type (WT) plants”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
Arabidopsis GbCCD8b overexpressors were more resistant than wild type.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- GbCCD8b-overexpressing plantse0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- GbCCD8be0
- Outcome
- resistant to Verticillium wilte0
- Direction
- more resistante0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both Arabidopsis GbCCD7- and GbCCD8b-overexpressing plants were more resistant to Verticillium wilt than wild-type (WT) plants”
e1“Verticillium wilt caused by Verticillium dahliae”
Claim · model-written summary
JA and ABA signaling genes were upregulated in wild type cotton roots following rac-GR24 and infection.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- rootse0
- Treatment
- rac-GR24 and V. dahliae infectione0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathwayse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathways, such as MYELOCYTOMATOSIS 2 (GbMYC2) and ABA-INSENSITIVE 5, respectively, were upregulated in the roots of WT cotton plants in responses to rac-GR24 and V. dahliae infection”
Claim · model-written summary
JA and ABA signaling genes were downregulated in GbCCD7 silenced cotton roots.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- rootse0
- Treatment
- GbCCD7-e0
- Gene
- GbCCD7e0
- Outcome
- several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathwayse1
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulated in the roots of both GbCCD7- and GbCCD8b-silenced cotton plants”
e1“several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathways”
Claim · model-written summary
JA and ABA signaling genes were downregulated in GbCCD8b silenced cotton roots.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- rootse0
- Treatment
- GbCCD8b-silencede0
- Gene
- GbCCD8be0
- Outcome
- several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathwayse1
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulated in the roots of both GbCCD7- and GbCCD8b-silenced cotton plants”
e1“several genes related to the jasmonic acid (JA)- and abscisic acid (ABA)-signaling pathways”
Claim · model-written summary
GbMYC2 suppressed GbCCD7 and GbCCD8b expression through promoter binding.
- Taxon
- cottonGossypium hirsutume1
- Gene
- GbMYC2e0
- Outcome
- expression of GbCCD7 and GbCCD8be0
- Direction
- suppressede0
- Qualifier
- binding to their promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbMYC2 suppressed the expression of GbCCD7 and GbCCD8b by binding to their promoters”
e1“in cotton through a negative feedback loop”
Claim · model-written summary
GbCCD7 silencing impaired infection induced ROS accumulation in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GbCCD7-e0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GbCCD7e0
- Outcome
- reactive oxygen species (ROS) accumulatione0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbCCD7- and GbCCD8b-silenced cotton plants were impaired in V. dahliae-induced reactive oxygen species (ROS) accumulation”
Claim · model-written summary
GbCCD8b silencing impaired infection induced ROS accumulation in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GbCCD8b-silencede0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GbCCD8be0
- Outcome
- reactive oxygen species (ROS) accumulatione0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbCCD7- and GbCCD8b-silenced cotton plants were impaired in V. dahliae-induced reactive oxygen species (ROS) accumulation”