Abstract
Regulation of plant hormonal status is one of the major targets of symbiotic signaling during nodule formation in legume plants. However, the genetic and hormonal networks that regulate transition to differentiation of nodules are not well-characterized in legume plants. Analysis of plant mutants forming nodules impaired in rhizobial infection allowed us to identify some regulators involved in the control of the later stages of nodule development. In the current work, we extend our earlier studies on the influence of exogenously applied cytokinin on the later stages of nodule morphogenesis using pea sym33 (ipd3/cyclops) mutants impaired in the gene encoding IPD3/CYCLOPS transcription factor. One of the noticeable effects of the influence of exogenously applied cytokinin on nodules in the sym33-3 mutant was an increasing size of these structures. Cytokinin treatment was shown to stimulate bacterial release and increase the percentage of infected cells in nodules. To explore the role of possible regulators of nodule differentiation, we performed searching in pea transcriptome. The transcriptome study in pea P. sativum revealed the importance of the CCS52 regulator, EFD transcription factor, SYMREM regulator, RSD, the MADS-domain/AGL, and SHORT INTERNODE/STYLISH gene families encoding transcription factors in the control of nodule differentiation. Analysis of the expression patterns was verified by real-time PCR in response to exogenously applied cytokinin treatment.
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Claims 5
Claim · model-written summary
Applied cytokinin increased nodule size in the sym33-3 pea mutant.
- Taxon
- Pea
Lathyrus oleraceust0 - Material
- sym33-3 mutante0
- Organ / stage
- nodulese0
- Treatment
- exogenously applied cytokinine0
- Outcome
- size of these structurese0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea Ipd3/Cyclops Mutant”
e0“One of the noticeable effects of the influence of exogenously applied cytokinin on nodules in the sym33-3 mutant was an increasing size of these structures.”
Claim · model-written summary
Cytokinin treatment stimulated bacterial release in nodules.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- nodulese0
- Treatment
- Cytokinin treatmente0
- Outcome
- bacterial releasee0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea Ipd3/Cyclops Mutant”
e0“Cytokinin treatment was shown to stimulate bacterial release and increase the percentage of infected cells in nodules.”
Claim · model-written summary
Cytokinin treatment increased the percentage of infected cells in nodules.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- Cytokinin treatmente0
- Outcome
- percentage of infected cells in nodulese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea Ipd3/Cyclops Mutant”
e0“Cytokinin treatment was shown to stimulate bacterial release and increase the percentage of infected cells in nodules.”
Claim · model-written summary
Transcriptome analysis implicated CCS52 regulator, EFD transcription factor, SYMREM regulator in nodule differentiation.
Verbatim
t0“Pea Ipd3/Cyclops Mutant”
e0“The transcriptome study in pea P. sativum revealed the importance of the CCS52 regulator, EFD transcription factor, SYMREM regulator, RSD, the MADS-domain/AGL, and SHORT INTERNODE/STYLISH gene families encoding transcription factors in the control of nodule differentiation.”
Claim · model-written summary
Transcriptome analysis implicated RSD, the MADS-domain/AGL, and SHORT INTERNODE/STYLISH gene families in nodule differentiation.
Verbatim
t0“Pea Ipd3/Cyclops Mutant”
e0“The transcriptome study in pea P. sativum revealed the importance of the CCS52 regulator, EFD transcription factor, SYMREM regulator, RSD, the MADS-domain/AGL, and SHORT INTERNODE/STYLISH gene families encoding transcription factors in the control of nodule differentiation.”
The same questions in other papers
class · disease · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
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