Abstract
Obtaining homozygous lines from transgenic plants is an important step for phenotypic evaluations, but the selection of homozygous plants is time-consuming and laborious. The process would be significantly shortened if anther or microspore culture could be completed in one generation. In this study, we obtained 24 homozygous doubled haploid (DH) transgenic plants entirely by microspore culture from one T0 transgenic plant overexpressing the gene HvPR1 (pathogenesis-related-1). Nine of the doubled haploids grew to maturity and produced seeds. qRCR (quantitative real-time PCR) validation showed that the HvPR1 gene was expressed differentially even among different DH1 plants (T2) from the same DH0 line (T1). Phenotyping analysis suggested that the overexpression of HvPR1 inhibited nitrogen use efficiency (NUE) only under low nitrogen treatment. The established method of producing homozygous transgenic lines will enable the rapid evaluation of transgenic lines for gene function studies and trait evaluation. As an example, the HvPR1 overexpression of DH lines also could be used for further analysis of NUE-related research in barley.
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Claims 5
Claim · model-written summary
Microspore culture produced 24 homozygous doubled haploid transgenic plants from one HvPR1-overexpressing T0 plant.
- Taxon
- Barley
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Hordeum vulgaret0 - Treatment
- microspore culturee0
- Gene
- HvPR1e0
- Outcome
- homozygous doubled haploid (DH) transgenic plantse0
- Value
- 24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“we obtained 24 homozygous doubled haploid (DH) transgenic plants entirely by microspore culture from one T0 transgenic plant overexpressing the gene HvPR1”
t0“Barley”
Claim · model-written summary
Nine doubled haploids matured and produced seeds.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- grew to maturity and produced seedse0
- Value
- Ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nine of the doubled haploids grew to maturity and produced seeds”
t0“Barley”
Claim · model-written summary
HvPR1 expression differed among DH1 plants from the same DH0 line.
- Taxon
- BarleyHordeum vulgaret0
- Material
- DH1 plantse0
- Gene
- HvPR1e0
- Outcome
- expressed differentiallye0
- Direction
- differentiallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the HvPR1 gene was expressed differentially even among different DH1 plants (T2) from the same DH0 line (T1)”
t0“Barley”
Claim · model-written summary
HvPR1 overexpression inhibited nitrogen use efficiency only under low nitrogen.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- overexpression of HvPR1e0
- Gene
- HvPR1e0
- Outcome
- nitrogen use efficiency (NUE)e0
- Direction
- inhibitede0
- Qualifier
- only under low nitrogen treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the overexpression of HvPR1 inhibited nitrogen use efficiency (NUE) only under low nitrogen treatment”
t0“Barley”
Claim · model-written summary
The authors state the method will enable rapid evaluation of transgenic lines for gene function and traits.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- rapid evaluation of transgenic lines for gene function studies and trait evaluatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The established method of producing homozygous transgenic lines will enable the rapid evaluation of transgenic lines for gene function studies and trait evaluation”
t0“Barley”