Abstract
This study aimed to determine the impact of three abiotic factors (Temperature, pH, and salinity) on the metabolic activities (macromolecules) and germination rate of Acinos alpinus subsp. Meridionalis (Satureja) seeds. In vitro, seed germination tests were performed in an aqueous medium. They were monitored as a function of time at different levels of temperature, NaCl concentration, and pH. The best germination rate (85.3%) was achieved at 15 °C and pH = 7. However, the germination was nil at a higher temperature (more than 25 °C), acidic pH (pH < 3.5), and higher NaCl concentration (more than >7.5 g L−1). Fourier transform infrared spectroscopy (FTIR) analysis showed an important variability of the chemical composition of germinated seeds. Indeed, the comparison of the absorbance peaks of chemical compounds in the treatments versus the control revealed significant differences in their concentrations and structures, which may justify why seeds fail to germinate under some extreme abiotic conditions. The results of this study are expected to serve as a guide for the protocols to be adopted in the ex situ conservation of this species.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Seed germination was highest at 15 °C and pH 7, reaching 85.3%.
- Taxon
- Acinos alpinus subsp. Meridionalis
Clinopodium alpinume1 - Organ / stage
- seedse1
- Treatment
- 15 °C and pH = 7e0
- Outcome
- germination ratee0
- Direction
- beste0
- Value
- 85.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best germination rate (85.3%) was achieved at 15 °C and pH = 7”
e1“Acinos alpinus subsp. Meridionalis (Satureja) seeds”
Claim · model-written summary
Germination was absent above 25 °C.
- Taxon
- Acinos alpinus subsp. MeridionalisClinopodium alpinume1
- Organ / stage
- seedse1
- Treatment
- higher temperature (more than 25 °C)e0
- Outcome
- germinatione0
- Direction
- nile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germination was nil at a higher temperature (more than 25 °C)”
e1“Acinos alpinus subsp. Meridionalis (Satureja) seeds”
Claim · model-written summary
Germination was absent below pH 3.5.
- Taxon
- Acinos alpinus subsp. MeridionalisClinopodium alpinume1
- Organ / stage
- seedse1
- Treatment
- acidic pH (pH < 3.5)e0
- Outcome
- germinatione0
- Direction
- nile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germination was nil at a higher temperature (more than 25 °C), acidic pH (pH < 3.5)”
e1“Acinos alpinus subsp. Meridionalis (Satureja) seeds”
Claim · model-written summary
Germination was absent at NaCl concentrations above 7.5 g L−1.
- Taxon
- Acinos alpinus subsp. MeridionalisClinopodium alpinume1
- Organ / stage
- seedse1
- Treatment
- higher NaCl concentration (more than >7.5 g L−1)e0
- Outcome
- germinatione0
- Direction
- nile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germination was nil at a higher temperature (more than 25 °C), acidic pH (pH < 3.5), and higher NaCl concentration (more than >7.5 g L−1)”
e1“Acinos alpinus subsp. Meridionalis (Satureja) seeds”
Claim · model-written summary
Germinated seeds varied substantially in chemical composition.
- Taxon
- Acinos alpinus subsp. MeridionalisClinopodium alpinume1
- Organ / stage
- germinated seedse0
- Outcome
- chemical compositione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“important variability of the chemical composition of germinated seeds”
e1“Acinos alpinus subsp. Meridionalis (Satureja) seeds”
Claim · model-written summary
Treatments significantly changed absorbance peaks representing compound concentrations and structures relative to controls.
- Taxon
- Acinos alpinus subsp. MeridionalisClinopodium alpinume1
- Treatment
- treatmentse0
- Outcome
- their concentrations and structurese0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“comparison of the absorbance peaks of chemical compounds in the treatments versus the control revealed significant differences in their concentrations and structures”
e1“Acinos alpinus subsp. Meridionalis (Satureja) seeds”