Abstract
Abstract Fungal pathogens pose a major threat to Cannabis sativa production, requiring safe and effective management procedures to control disease. Chitin and chitosan are natural molecules that elicit plant defense responses. Investigation of their effects on C. sativa will advance understanding of plant responses towards elicitors and provide a potential pathway to enhance plant resistance against diseases. Plants were grown in the in vitro Root‐TRAPR system and treated with colloidal chitin and chitosan. Plant morphology was monitored, then plant tissues and exudates were collected for enzymatic activity assays, phytohormone quantification, qPCR analysis and proteomics profiling. Chitosan treatments showed increased total chitinase activity and expression of pathogenesis‐related (PR) genes by 3–5 times in the root tissues. In the exudates, total peroxidase and chitinase activities and levels of defense proteins such as PR protein 1 and endochitinase 2 were increased. Shoot development was unaffected, but root development was inhibited after chitosan exposure. In contrast, chitin treatments had no significant impact on any defense parameters, including enzymatic activities, hormone quantities, gene expression levels and root secreted proteins. These results indicate that colloidal chitosan, significantly enhancing defense responses in C. sativa root system, could be used as a potential elicitor, particularly in hydroponic scenarios to manage crop diseases.
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Claims 7
Claim · model-written summary
Chitosan increased root chitinase activity and PR gene expression three to fivefold.
- Taxon
- Cannabis sativa
Cannabis sativat0 - Organ / stage
- root tissuese0
- Treatment
- Chitosane0
- Outcome
- total chitinase activity and expression of pathogenesis‐related (PR) genese0
- Direction
- increasede0
- Value
- 3–5 timese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“Chitosan treatments showed increased total chitinase activity and expression of pathogenesis‐related (PR) genes by 3–5 times in the root tissues”
Claim · model-written summary
Chitosan increased peroxidase and chitinase activities in root exudates.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- exudatese0
- Treatment
- chitosane1
- Outcome
- total peroxidase and chitinase activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“In the exudates, total peroxidase and chitinase activities and levels of defense proteins such as PR protein 1 and endochitinase 2 were increased”
e1“after chitosan exposure”
Claim · model-written summary
Chitosan increased defense protein levels in exudates.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- exudatese0
- Treatment
- chitosane1
- Outcome
- levels of defense proteins such as PR protein 1 and endochitinase 2e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“In the exudates, total peroxidase and chitinase activities and levels of defense proteins such as PR protein 1 and endochitinase 2 were increased”
e1“after chitosan exposure”
Claim · model-written summary
Chitosan did not affect shoot development.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- chitosan exposuree0
- Outcome
- Shoot developmente0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“Shoot development was unaffected, but root development was inhibited after chitosan exposure”
Claim · model-written summary
Chitosan inhibited root development.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- chitosan exposuree0
- Outcome
- root developmente0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“Shoot development was unaffected, but root development was inhibited after chitosan exposure”
Claim · model-written summary
Chitin had no significant impact on defense enzymes, hormones, gene expression or root secreted proteins.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- chitin treatmentse0
- Outcome
- defense parameterse0
- Direction
- no significant impacte0
- Qualifier
- enzymatic activities, hormone quantities, gene expression levels and root secreted proteinse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“chitin treatments had no significant impact on any defense parameters, including enzymatic activities, hormone quantities, gene expression levels and root secreted proteins”
Claim · model-written summary
The authors suggest colloidal chitosan as an elicitor for hydroponic disease management.
- Taxon
- C. sativaCannabis sativae0
- Treatment
- colloidal chitosane0
- Outcome
- could be used as a potential elicitore0
- Qualifier
- particularly in hydroponic scenarios to manage crop diseasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e0“colloidal chitosan, significantly enhancing defense responses in C. sativa root system, could be used as a potential elicitor, particularly in hydroponic scenarios to manage crop diseases”
The same questions in other papers
chitosan · growth · Cannabis sativaResults agree
1 paper in the same direction as this paper
- negative
chitosan · disease · Cannabis sativaResults agree
1 paper in the same direction as this paper
- positive
chitosan · physiology: antioxidant enzymes · Cannabis sativaResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.