Abstract
BACKGROUND: As climate change events become more frequent, drought is an increasing threat to agricultural production and food security. Crop rhizosphere microbiome and root exudates are critical regulators for drought adaptation, yet our understanding on the rhizosphere bacterial communities and root exudate composition as affected by drought stress is far from complete. In this study, we performed 16S rRNA gene amplicon sequencing and widely targeted metabolomic analysis of rhizosphere soil and root exudates from two contrasting rice genotypes (Nipponbare and Luodao 998) exposed to drought stress. RESULTS: A reduction in plant phenotypes was observed under drought, and the inhibition was greater for roots than for shoots. Additionally, drought exerted a negligible effect on the alpha diversity of rhizosphere bacterial communities, but obviously altered their composition. In particular, drought led to a significant enrichment of Actinobacteria but a decrease in Firmicutes. We also found that abscisic acid in root exudates was clearly higher under drought, whereas lower jasmonic acid and L-cystine concentrations. As for plant genotypes, variations in plant traits of the drought-tolerant genotype Luodao 998 after drought were smaller than those of Nipponbare. Interestingly, drought triggered an increase in Bacillus, as well as an upregulation of most organic acids and a downregulation of all amino acids in Luodao 998. Notably, both Procrustes analysis and Mantel test demonstrated that rhizosphere microbiome and root exudate metabolomic profiles were highly correlated. A number of differentially abundant genera responded to drought and genotype, including Streptomyces, Bacillus and some members of Actinobacteria, were significantly associated with organic acid and amino acid contents in root exudates. Further soil incubation experiments showed that Streptomyces was regulated by abscisic acid and jasmonic acid under drought. CONCLUSIONS: Our results reveal that both drought and genotype drive changes in the compositions of rice rhizosphere bacterial communities and root exudates under the greenhouse condition, and that organic acid exudation and suppression of amino acid exudation to select specific rhizosphere bacterial communities may be an important strategy for rice to cope with drought. These findings have important implications for improving the adaptability of rice to drought from the perspective of plant-microbe interactions.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 16
Claim · model-written summary
Drought reduced rice plant phenotypes.
- Taxon
- Rice
Oryza sativat0 - Treatment
- droughte1
- Outcome
- plant phenotypese1
- Direction
- reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e1“A reduction in plant phenotypes was observed under drought, and the inhibition was greater for roots than for shoots”
Claim · model-written summary
Drought inhibited rice roots more than shoots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse1
- Treatment
- droughte1
- Outcome
- inhibitione1
- Direction
- greatere1
- Comparator
- shootse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e1“A reduction in plant phenotypes was observed under drought, and the inhibition was greater for roots than for shoots”
Claim · model-written summary
Drought had a negligible effect on the alpha diversity of rhizosphere bacterial communities of rice.
- Taxon
- RiceOryza sativat0
- Treatment
- droughte2
- Interactor
- rhizosphere bacterial communitiese2symbiont
- Outcome
- alpha diversity of rhizosphere bacterial communitiese2
- Direction
- negligible effecte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e2“drought exerted a negligible effect on the alpha diversity of rhizosphere bacterial communities, but obviously altered their composition”
Claim · model-written summary
Drought altered the composition of rhizosphere bacterial communities of rice.
- Taxon
- RiceOryza sativat0
- Treatment
- droughte2
- Interactor
- rhizosphere bacterial communitiese2symbiont
- Outcome
- compositione2
- Direction
- alterede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e2“drought exerted a negligible effect on the alpha diversity of rhizosphere bacterial communities, but obviously altered their composition”
Claim · model-written summary
Drought significantly enriched Actinobacteria in the rice rhizosphere.
- Taxon
- RiceOryza sativat0
- Treatment
- droughte3
- Interactor
- ActinobacteriaActinomycetotae3symbiont
- Outcome
- Actinobacteriae3
- Direction
- enrichmente3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e3“drought led to a significant enrichment of Actinobacteria but a decrease in Firmicutes”
Claim · model-written summary
Drought decreased Firmicutes in the rice rhizosphere.
- Taxon
- RiceOryza sativat0
- Treatment
- droughte3
- Outcome
- Firmicutese3
- Direction
- decreasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e3“drought led to a significant enrichment of Actinobacteria but a decrease in Firmicutes”
Claim · model-written summary
Drought made abscisic acid in rice root exudates clearly higher.
- Taxon
- RiceOryza sativat0
- Treatment
- droughte4
- Compound
- abscisic acide4
- Outcome
- abscisic acid in root exudatese4
- Direction
- highere4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e4“We also found that abscisic acid in root exudates was clearly higher under drought, whereas lower jasmonic acid and L-cystine concentrations”
Claim · model-written summary
Drought made jasmonic acid and L-cystine concentrations in rice root exudates lower.
- Taxon
- RiceOryza sativat0
- Treatment
- droughte4
- Compound
- jasmonic acide4
- Outcome
- jasmonic acid and L-cystine concentrationse4
- Direction
- lowere4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e4“We also found that abscisic acid in root exudates was clearly higher under drought, whereas lower jasmonic acid and L-cystine concentrations”
Claim · model-written summary
Drought increased Bacillus in the rhizosphere of rice genotype Luodao 998.
- Taxon
- RiceOryza sativat0
- Material
- Luodao 998e5
- Treatment
- droughte5
- Interactor
- Bacilluse5symbiont
- Outcome
- Bacilluse5
- Direction
- increasee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e5“drought triggered an increase in Bacillus, as well as an upregulation of most organic acids and a downregulation of all amino acids in Luodao 998”
Claim · model-written summary
Drought upregulated most organic acids in root exudates of rice genotype Luodao 998.
- Taxon
- RiceOryza sativat0
- Material
- Luodao 998e5
- Treatment
- droughte5
- Outcome
- most organic acidse5
- Direction
- upregulatione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e5“drought triggered an increase in Bacillus, as well as an upregulation of most organic acids and a downregulation of all amino acids in Luodao 998”
Claim · model-written summary
Drought downregulated all amino acids in root exudates of rice genotype Luodao 998.
- Taxon
- RiceOryza sativat0
- Material
- Luodao 998e5
- Treatment
- droughte5
- Outcome
- all amino acidse5
- Direction
- downregulatione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e5“drought triggered an increase in Bacillus, as well as an upregulation of most organic acids and a downregulation of all amino acids in Luodao 998”
Claim · model-written summary
Plant traits of the drought-tolerant genotype Luodao 998 varied less after drought than those of Nipponbare.
- Taxon
- RiceOryza sativat0
- Material
- Luodao 998e6
- Treatment
- droughte6
- Outcome
- variations in plant traitse6
- Direction
- smallere6
- Comparator
- Nipponbaree6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e6“As for plant genotypes, variations in plant traits of the drought-tolerant genotype Luodao 998 after drought were smaller than those of Nipponbare”
Claim · model-written summary
Rhizosphere microbiome and root exudate metabolomic profiles of rice were highly correlated.
- Taxon
- RiceOryza sativat0
- Interactor
- rhizosphere microbiomee7symbiont
- Outcome
- rhizosphere microbiome and root exudate metabolomic profilese7
- Direction
- highly correlatede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e7“Notably, both Procrustes analysis and Mantel test demonstrated that rhizosphere microbiome and root exudate metabolomic profiles were highly correlated”
Claim · model-written summary
Streptomyces and Bacillus were significantly associated with organic acid and amino acid contents in rice root exudates.
- Taxon
- RiceOryza sativat0
- Interactor
- Streptomyces
Streptomycese2symbiont - Second interactor
- Bacilluse2symbiont
- Outcome
- organic acid and amino acid contents in root exudatese2
- Direction
- significantly associatede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e2“A number of differentially abundant genera responded to drought and genotype, including Streptomyces, Bacillus and some members of Actinobacteria, were significantly associated with organic acid and amino acid contents in root exudates”
Claim · model-written summary
Streptomyces was regulated by abscisic acid and jasmonic acid under drought in soil incubation.
- Taxon
- RiceOryza sativat0
- Interactor
- StreptomycesStreptomycese2symbiont
- Compound
- abscisic acid and jasmonic acide2
- Outcome
- regulatede2
- Qualifier
- under droughte2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e2“Further soil incubation experiments showed that Streptomyces was regulated by abscisic acid and jasmonic acid under drought”
Claim · model-written summary
Organic acid exudation and suppression of amino acid exudation may select specific rhizosphere bacterial communities that help rice cope with drought.
- Taxon
- RiceOryza sativat0
- Treatment
- suppression of amino acid exudatione2
- Compound
- organic acide2
- Outcome
- cope with droughte2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)”
e2“organic acid exudation and suppression of amino acid exudation to select specific rhizosphere bacterial communities may be an important strategy for rice to cope with drought”
The same questions in other papers
drought · soil: biology · Oryza sativa (rice)Positive and negative results
6 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →drought · disease · Oryza sativa (rice)Positive and negative results
6 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.