Abstract
The effect of exogenous γ-aminobutyric acid (GABA) and GABA synthesis inhibitor 3-mercaptopropionic acid (3-MP) on GABA metabolism and quality in fresh-cut pumpkin was investigated, and the possible mechanism was studied. The results indicated that exogenous GABA induced the accumulation of endogenous GABA and promoted the conversion of glutamic acid to GABA in fresh-cut pumpkin through the catalytic conversion of highly active glutamate decarboxylase (GAD). The 3-MP-treated pumpkin retained lower GABA content than the control, and the activities of GAD and GABA transaminase (GABA-T) were inhibited. The GABA-treated pumpkins possessed relatively lower polyamines (putrescine, spermine and spermidine) contents in comparison with the control during storage. 3-MP treatment restrained the activities of polyamine oxidase (PAO), diamine oxidase (DAO) and amino aldehyde decarboxylase (AMADH). These results demonstrated that GABA treatment could activate the polyamine degradation pathway and promote GABA enrichment in pumpkin. Meanwhile, no significant differences in maximum colony count, color, β-carotene content and soluble solids content were observed among treatments during storage, which suggested that exogenous GABA had little effect on the quality of fresh-cut pumpkin.
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Claims 8
Claim · model-written summary
Exogenous GABA increased endogenous GABA accumulation in fresh-cut pumpkin.
- Taxon
- fresh-cut pumpkine0
- Treatment
- exogenous GABAe0
- Outcome
- accumulation of endogenous GABAe0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“exogenous GABA induced the accumulation of endogenous GABA and promoted the conversion of glutamic acid to GABA in fresh-cut pumpkin”
Claim · model-written summary
Exogenous GABA promoted conversion of glutamic acid to GABA through glutamate decarboxylase.
Verbatim
e0“promoted the conversion of glutamic acid to GABA in fresh-cut pumpkin through the catalytic conversion of highly active glutamate decarboxylase (GAD)”
Claim · model-written summary
3-MP-treated pumpkin had lower GABA content than controls.
Verbatim
e0“The 3-MP-treated pumpkin retained lower GABA content than the control”
Claim · model-written summary
3-MP inhibited GAD and GABA-T activities in pumpkin.
Not reportedTaxonTreatmentComparator
Verbatim
e0“the activities of GAD and GABA transaminase (GABA-T) were inhibited.”
Claim · model-written summary
GABA-treated pumpkins had lower putrescine, spermine and spermidine contents than controls during storage.
- Treatment
- GABAe0
- Outcome
- polyamines (putrescine, spermine and spermidine) contentse0
- Direction
- lowere0
- Qualifier
- during storagee0
- Comparator
- controle0
CucurbitaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GABA-treated pumpkins possessed relatively lower polyamines (putrescine, spermine and spermidine) contents in comparison with the control during storage.”
Claim · model-written summary
3-MP restrained PAO, DAO and AMADH activities.
- Treatment
- 3-MPe0
- Outcome
- activities of polyamine oxidase (PAO), diamine oxidase (DAO) and amino aldehyde decarboxylase (AMADH)e0
- Direction
- restrainede0
Not reportedTaxonComparator
Verbatim
e0“3-MP treatment restrained the activities of polyamine oxidase (PAO), diamine oxidase (DAO) and amino aldehyde decarboxylase (AMADH).”
Claim · model-written summary
GABA treatment was interpreted to activate polyamine degradation and enrich pumpkin GABA.
Verbatim
e0“GABA treatment could activate the polyamine degradation pathway and promote GABA enrichment in pumpkin.”
Claim · model-written summary
Maximum colony count, color, β-carotene and soluble solids did not differ significantly among treatments during storage.
- Treatment
- treatmentse0
- Outcome
- maximum colony count, color, β-carotene contente0
- Direction
- no significant differencese0
Not reportedTaxonComparator
Verbatim
e0“no significant differences in maximum colony count, color, β-carotene content and soluble solids content were observed among treatments during storage”
The same questions in other papers
gamma-aminobutyric acid · molecular: metabolites · Cucurbita (pumpkins)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.