Paper · 2023 · Biology

Phytochemical Extracts of Dittrichia viscosa (L.) Greuter from Agroecological Systems: Seed Antigerminative Properties and Effectiveness in Counteracting Alternaria Leaf Spot Disease on Baby-Leaf Spinach

Catello Pane; Gelsomina Manganiello; Antonella Vitti; et al.

7
claims
9
verbatim quotes
11
citations
3
research areas
✓
Open access · cc-by

Abstract

Dittrichia viscosa (L.) Greuter subsp. viscosa (Asteraceae) is a perennial species naturally distributed in arid and marginal areas whose agroecological cultivation could be a useful innovation to produce quality biomass to extract phenolic-rich phytochemical blends. Here, biomass-yield trends were profiled at different growth stages under direct cropping, and inflorescences, leaves, and stems were submitted to water extraction and hydrodistillation. Then, four extracts were investigated for their biological activities in invitro and in planta assays. Extracts inhibited cress (Lepidium sativum)- and radish (Raphanus sativus)-seed germination and root elongation. All samples showed dose-dependent antifungal activity in the plate experiments, inhibiting up to 65% of the growth of the fungal pathogen Alternaria alternata, a leaf-spot disease agent of baby spinach (Spinacea oleracea). However, only the extracts from dried green parts and fresh inflorescences at the highest concentration significantly reduced (54%) the extent of Alternaria necrosis on baby spinach. UHPLC-HRMS/MS analysis revealed that the main specialized metabolites of the extracts are caffeoyl quinic acids, methoxylated flavonoids, sesquiterpene compounds such as tomentosin, and dicarboxylic acids, which may explain the observed bioactivity. Plant extracts obtained through sustainable methodology can be effective in biological agricultural applications.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Extracts inhibited cress seed germination and root elongation.

Taxon
cresse0
Treatment
Extractse0
Outcome
seed germination and root elongatione0
Direction
inhibitede0

Not reportedComparator

Verbatim

e0

“Extracts inhibited cress (Lepidium sativum)- and radish (Raphanus sativus)-seed germination and root elongation.”

✓ located verbatim in the source · abstract, characters 558–670 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Extracts inhibited radish seed germination and root elongation.

Treatment
Extractse0
Outcome
seed germination and root elongatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Extracts inhibited cress (Lepidium sativum)- and radish (Raphanus sativus)-seed germination and root elongation.”

✓ located verbatim in the source · abstract, characters 558–670 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All samples inhibited Alternaria alternata growth by up to 65% in plate experiments in a dose dependent manner.

Taxon
baby spinache0
Treatment
All samplese0
Interactor
Alternaria alternataAlternaria alternatae0pathogen
Outcome
growth of the fungal pathogen Alternaria alternatae0
Direction
inhibitinge0
Value
65%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Alternaria alternata
Alternaria alternata · Interactor · picture from its Wikimedia Commons categoryMarta Hiromi Taniwaki, Secretaria de Agricultura e Abastecimento · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“All samples showed dose-dependent antifungal activity in the plate experiments, inhibiting up to 65% of the growth of the fungal pathogen Alternaria alternata, a leaf-spot disease agent of baby spinach (Spinacea oleracea).”

✓ located verbatim in the source · abstract, characters 671–893 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Dried green part extracts at the highest concentration significantly reduced Alternaria necrosis on baby spinach by 54%.

Taxon
baby spinache0
Treatment
extracts from dried green partse0
Interactor
AlternariaAlternariae0pathogen
Outcome
extent of Alternaria necrosise0
Direction
reducede0
Value
54%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Alternaria
Alternaria · Interactor · picture of the genus (one of its species)http://phil.cdc.gov/phil_images/20030612/9/PHIL_3963_lores.jpg · Public domain · Wikimedia Commons ↗

Verbatim

e0

“only the extracts from dried green parts and fresh inflorescences at the highest concentration significantly reduced (54%) the extent of Alternaria necrosis on baby spinach”

✓ located verbatim in the source · abstract, characters 903–1075 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fresh inflorescence extracts at the highest concentration significantly reduced Alternaria necrosis on baby spinach by 54%.

Taxon
baby spinache0
Treatment
extracts from dried green parts and fresh inflorescencese0
Interactor
AlternariaAlternariae0pathogen
Outcome
extent of Alternaria necrosise0
Direction
reducede0
Value
54%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Alternaria
Alternaria · Interactor · picture of the genus (one of its species)http://phil.cdc.gov/phil_images/20030612/9/PHIL_3963_lores.jpg · Public domain · Wikimedia Commons ↗

Verbatim

e0

“only the extracts from dried green parts and fresh inflorescences at the highest concentration significantly reduced (54%) the extent of Alternaria necrosis on baby spinach”

✓ located verbatim in the source · abstract, characters 903–1075 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The extracts contained caffeoyl quinic acids, methoxylated flavonoids, and sesquiterpene compounds such as tomentosin.

Taxon
Dittrichia viscosa (L.) GreuterDittrichia viscosat0
Outcome
caffeoyl quinic acids, methoxylated flavonoids, sesquiterpene compounds such as tomentosine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dittrichia viscosa
Dittrichia viscosa · TaxonJavier martin · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the main specialized metabolites of the extracts are caffeoyl quinic acids, methoxylated flavonoids, sesquiterpene compounds such as tomentosin, and dicarboxylic acids”

✓ located verbatim in the source · abstract, characters 1114–1281 · show in text ↑
t0

“Dittrichia viscosa (L.) Greuter”

✓ located verbatim in the source · title, characters 26–57 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Dicarboxylic acids were among the main specialized metabolites of the extracts.

Taxon
Dittrichia viscosa (L.) GreuterDittrichia viscosat0
Outcome
dicarboxylic acidse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dittrichia viscosa
Dittrichia viscosa · TaxonJavier martin · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the main specialized metabolites of the extracts are caffeoyl quinic acids, methoxylated flavonoids, sesquiterpene compounds such as tomentosin, and dicarboxylic acids”

✓ located verbatim in the source · abstract, characters 1114–1281 · show in text ↑
t0

“Dittrichia viscosa (L.) Greuter”

✓ located verbatim in the source · title, characters 26–57 · show in text ↑

botanical · growth · Raphanus sativus (radish)Positive and negative results

152 papers: 53 positive, 99 negative or no effect

98 papers in the same direction as this paper · 53 in the opposite direction

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.