Abstract
Cell type-specific mapping of element distribution is critical to fully understand how roots partition nutrients and toxic elements with aboveground parts. In this study, we developed a method that combines fluorescence-activated cell sorting (FACS) with inductively coupled plasma mass spectrometry (ICP-MS) to assess the ionome of different cell populations within Arabidopsis thaliana roots. The method reveals that most elements exhibit a radial concentration gradient increasing from the rhizodermis to inner cell layers, and detected previously unknown ionomic changes resulting from perturbed xylem loading processes. With this approach, we also identify a strong accumulation of manganese in trichoblasts of iron-deficient roots. We demonstrate that confining manganese sequestration in trichoblasts but not in endodermal cells efficiently retains manganese in roots, therefore preventing toxicity in shoots. These results indicate the existence of cell type-specific constraints for efficient metal sequestration in roots. Thus, our approach opens an avenue to investigate element compartmentation and transport pathways in plants.
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Claims 6
Claim · model-written summary
FACS combined with ICP-MS assessed ionomes of distinct Arabidopsis root cell populations.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Outcome
- ionome of different cell populationse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we developed a method that combines fluorescence-activated cell sorting (FACS) with inductively coupled plasma mass spectrometry (ICP-MS) to assess the ionome of different cell populations within Arabidopsis thaliana roots”
Claim · model-written summary
Most elements increased radially from rhizodermis toward inner root layers.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- rootst0
- Outcome
- radial concentration gradiente0
- Direction
- increasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most elements exhibit a radial concentration gradient increasing from the rhizodermis to inner cell layers”
t0“Arabidopsis thaliana roots”
Claim · model-written summary
The method detected previously unknown ionomic changes following perturbed xylem loading.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- perturbed xylem loading processese0
- Outcome
- ionomic changese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“detected previously unknown ionomic changes resulting from perturbed xylem loading processes”
t0“Arabidopsis thaliana roots”
Claim · model-written summary
Manganese strongly accumulated in trichoblasts of iron-deficient roots.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- trichoblastse0
- Outcome
- accumulation of manganesee0
- Direction
- strong accumulatione0
- Qualifier
- iron-deficient rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong accumulation of manganese in trichoblasts of iron-deficient roots”
t0“Arabidopsis thaliana roots”
Claim · model-written summary
Confining manganese sequestration to trichoblasts retained manganese in roots.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- confining manganese sequestration in trichoblastse0
- Outcome
- manganese in rootse0
- Direction
- retainse0
- Comparator
- endodermal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confining manganese sequestration in trichoblasts but not in endodermal cells efficiently retains manganese in roots”
t0“Arabidopsis thaliana roots”
Claim · model-written summary
Confining manganese sequestration to trichoblasts prevented shoot toxicity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- confining manganese sequestration in trichoblastse0
- Outcome
- toxicity in shootse0
- Direction
- preventinge0
- Comparator
- endodermal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confining manganese sequestration in trichoblasts but not in endodermal cells efficiently retains manganese in roots, therefore preventing toxicity in shoots”
t0“Arabidopsis thaliana roots”
The same questions in other papers
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1 in the opposite direction
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.