Paper · 2023 · Pakistan Journal of Phytopathology

IDENTIFICATION OF THE MAIN PATHOGENIC FUNGUS OF ROOT ROT OF DATE PALM OFFSHOOTS (PHOENIX DICTYLIFERA L.), AND THE ANTAGONISTIC EFFECT OF TRICHODERMA HARZIANUM IN VITRO

Naji Jassim; Mahmood O. Jaaafer

9
claims
17
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by

Abstract

Root rot is a significant underground disease in date palms (Phoenix dactylifera L.) caused by pathogenic soil-borne fungus Fusarium oxysporum. Polymerase chain reaction (PCR) amplification was performed, followed by analysis of the sequences of nitrogen bases of the products using the program BLAST (Basic Local Alignment Search Tool). The results revealed that most pathogenic isolates were belonging to F. oxysporum, F. proliferatum S1, F. proliferatum S2, and F. fujikuroi. The selected isolates of Fusarium spp. were sequenced. The result of a pathogenicity assay shows that all the assayed isolated fungi were pathogenic to date palm plantlets at different percentages, and the main causative pathogenic fungus was F. oxysporum with disease severity (DS) of 96% and disease incidence (DI) of 96.6%. An attempt was made to find the most appropriate bioagents that can protect date palm offshoots (P. dactylfera L.) from certain soil-borne fungal diseases. The antifungal efficacy of Trichoderma harzianum was tested in vitro, where it inhibited mycelial radial growth of F. oxysporum by 87% on PDA medium. The results showed there was no reduction in mycelial growth of T. harzianum at all the different concentrations of NaCl tested under in vitro growth conditions compared with control concentrations (0 dSm).

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Claims 9

Observationobservedoriginal resultmeasured

Claim · model-written summary

Pathogenic isolates were identified as F. oxysporum.

Taxon
DATE PALMPhoenix dactyliferat0
Interactor
F. oxysporumFusarium oxysporume0pathogen
Outcome
F. oxysporume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“most pathogenic isolates were belonging to F. oxysporum, F. proliferatum S1, F. proliferatum S2, and F. fujikuroi”

✓ located verbatim in the source · abstract, characters 364–477 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pathogenic isolates were identified as F. proliferatum S1.

Taxon
DATE PALMPhoenix dactyliferat0
Interactor
F. proliferatum S1Fusarium proliferatume0pathogen
Outcome
F. proliferatum S1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“most pathogenic isolates were belonging to F. oxysporum, F. proliferatum S1, F. proliferatum S2, and F. fujikuroi”

✓ located verbatim in the source · abstract, characters 364–477 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pathogenic isolates were identified as F. proliferatum S2.

Taxon
DATE PALMPhoenix dactyliferat0
Interactor
F. proliferatum S2Fusarium proliferatume0pathogen
Outcome
F. proliferatum S2e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“most pathogenic isolates were belonging to F. oxysporum, F. proliferatum S1, F. proliferatum S2, and F. fujikuroi”

✓ located verbatim in the source · abstract, characters 364–477 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pathogenic isolates were identified as F. fujikuroi.

Taxon
DATE PALMPhoenix dactyliferat0
Interactor
F. fujikuroiFusarium fujikuroie0pathogen
Outcome
F. fujikuroie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium fujikuroi
Fusarium fujikuroi · InteractorClemson University - USDA Cooperative Extension Slide Series, Bugwood.org · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“most pathogenic isolates were belonging to F. oxysporum, F. proliferatum S1, F. proliferatum S2, and F. fujikuroi”

✓ located verbatim in the source · abstract, characters 364–477 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

All tested fungal isolates were pathogenic to date palm plantlets at different percentages.

Taxon
date palmPhoenix dactyliferae0
Organ / stage
plantletse0
Interactor
assayed isolated fungie0pathogen
Outcome
pathogenic to date palm plantletse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“all the assayed isolated fungi were pathogenic to date palm plantlets at different percentages”

✓ located verbatim in the source · abstract, characters 581–675 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

F. oxysporum caused 96% disease severity in date palm plantlets.

Taxon
DATE PALMPhoenix dactyliferat0
Interactor
F. oxysporumFusarium oxysporume0pathogen
Outcome
disease severity (DS)e0
Value
96%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the main causative pathogenic fungus was F. oxysporum with disease severity (DS) of 96% and disease incidence (DI) of 96.6%”

✓ located verbatim in the source · abstract, characters 681–804 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

F. oxysporum caused 96.6% disease incidence in date palm plantlets.

Taxon
DATE PALMPhoenix dactyliferat0
Interactor
F. oxysporumFusarium oxysporume0pathogen
Outcome
disease incidence (DI)e0
Value
96.6%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the main causative pathogenic fungus was F. oxysporum with disease severity (DS) of 96% and disease incidence (DI) of 96.6%”

✓ located verbatim in the source · abstract, characters 681–804 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma harzianum inhibited F. oxysporum radial growth by 87% in vitro.

Taxon
DATE PALMPhoenix dactyliferat0
Treatment
Trichoderma harzianume0
Interactor
F. oxysporumFusarium oxysporume0pathogen
Second interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
mycelial radial growth of F. oxysporume0
Direction
inhibitede0
Value
87%e0
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Trichoderma harzianum was tested in vitro, where it inhibited mycelial radial growth of F. oxysporum by 87% on PDA medium”

✓ located verbatim in the source · abstract, characters 989–1110 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

NaCl concentrations did not reduce T. harzianum mycelial growth relative to control.

Taxon
DATE PALMPhoenix dactyliferat0
Treatment
NaCle0
Interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
mycelial growth of T. harzianume0
Direction
no reductione0
Comparator
control concentrations (0 dSm)e0
Setting
in vitro growth conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“there was no reduction in mycelial growth of T. harzianum at all the different concentrations of NaCl tested under in vitro growth conditions compared with control concentrations (0 dSm)”

✓ located verbatim in the source · abstract, characters 1131–1317 · show in text ↑
t0

“DATE PALM OFFSHOOTS”

✓ located verbatim in the source · title, characters 60–79 · show in text ↑

sodium chloride · Trichoderma harzianum · Phoenix dactylifera (date palm)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.