Abstract
Cotton is one of the most important economic crops in the world and is a major source of fiber in the textile industry. Strigolactones (SLs) are a class of carotenoid-derived plant hormones involved in many processes of plant growth and development; however, SL functions in fiber development remain largely unknown. Here, we found that the endogenous SLs were significantly higher in fibers 20 days post-anthesis (DPA). Exogenous SLs significantly increased fiber length and cell wall thickness. Furthermore, we cloned three key SLS biosynthetic genes, namely GhD27, GhMAX3, and GhMAX4, which were highly expressed in fibers, and subcellular localization analyses revealed that GhD27, GhMAX3, and GhMAX4 were localized in the chloroplast. The exogenous expression of GhD27, GhMAX3, and GhMAX4 complemented the physiological phenotypes of d27, max3, and max4 mutations in Arabidopsis, respectively. Knockdown of GhD27, GhMAX3, and GhMAX4 in cotton resulted in an increased number of axillary buds and leaves, decreased fiber length, and significantly reduced fiber thickness. These findings revealed that SLs participate in plant growth, fiber elongation, and secondary cell wall formation in cotton. These results provide new and effective genetic resources for improving cotton fiber yield and plant architecture.
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Claims 8
Claim · model-written summary
Endogenous strigolactones were significantly higher in cotton fibers at 20 DPA.
- Taxon
- cotton
Gossypium hirsutumt0 - Organ / stage
- fibers 20 days post-anthesis (DPA)e0
- Outcome
- endogenous SLse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the endogenous SLs were significantly higher in fibers 20 days post-anthesis (DPA)”
t0“cotton fiber elongation and secondary cell wall thickening”
Claim · model-written summary
Exogenous strigolactones increased cotton fiber length and cell wall thickness.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Exogenous SLse0
- Outcome
- fiber length and cell wall thicknesse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous SLs significantly increased fiber length and cell wall thickness.”
t0“cotton fiber elongation and secondary cell wall thickening”
Claim · model-written summary
GhD27, GhMAX3 and GhMAX4 were highly expressed in cotton fibers.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- fiberse0
- Gene
- GhD27, GhMAX3, and GhMAX4e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhD27, GhMAX3, and GhMAX4, which were highly expressed in fibers”
t0“cotton fiber elongation and secondary cell wall thickening”
Claim · model-written summary
GhD27, GhMAX3 and GhMAX4 localized to chloroplasts.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhD27, GhMAX3, and GhMAX4e0
- Outcome
- localized in the chloroplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhD27, GhMAX3, and GhMAX4 were localized in the chloroplast”
t0“cotton fiber elongation and secondary cell wall thickening”
Claim · model-written summary
Expression of the three cotton genes complemented corresponding Arabidopsis mutant phenotypes.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- exogenous expression of GhD27, GhMAX3, and GhMAX4e0
- Gene
- GhD27, GhMAX3, and GhMAX4e0
- Outcome
- complemented the physiological phenotypese0
- Comparator
- d27, max3, and max4 mutationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The exogenous expression of GhD27, GhMAX3, and GhMAX4 complemented the physiological phenotypes of d27, max3, and max4 mutations in Arabidopsis, respectively.”
Claim · model-written summary
Knockdown of three genes increased cotton axillary buds and leaves.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Knockdown of GhD27, GhMAX3, and GhMAX4e0
- Gene
- GhD27, GhMAX3, and GhMAX4e0
- Outcome
- number of axillary buds and leavese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of GhD27, GhMAX3, and GhMAX4 in cotton resulted in an increased number of axillary buds and leaves”
Claim · model-written summary
Knockdown of three genes decreased cotton fiber length.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Knockdown of GhD27, GhMAX3, and GhMAX4e0
- Gene
- GhD27, GhMAX3, and GhMAX4e0
- Outcome
- fiber lengthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of GhD27, GhMAX3, and GhMAX4 in cotton resulted in an increased number of axillary buds and leaves, decreased fiber length”
Claim · model-written summary
Knockdown of three genes reduced cotton fiber thickness.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Knockdown of GhD27, GhMAX3, and GhMAX4e0
- Gene
- GhD27, GhMAX3, and GhMAX4e0
- Outcome
- fiber thicknesse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of GhD27, GhMAX3, and GhMAX4 in cotton resulted in an increased number of axillary buds and leaves, decreased fiber length, and significantly reduced fiber thickness”
The same questions in other papers
class · cytology · Gossypium hirsutum (cotton)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.