Abstract
Powdery mildew , caused by Blumeria graminis f. sp. tritici ( Bgt ), is a destructive disease affecting wheat crops worldwide. Functional genes can be activated in response to Bgt inoculations. Calcineurin B-like protein (CBL) together with CBL-interacting protein kinase (CIPK) forms the CBL–CIPK protein complex that participates in Ca 2+ sensor kinase–related signaling pathways responding to abiotic and biotic stresses . In this study, we performed a genome-wide screening and identified 27 CIPK subfamilies (123 CIPK transcripts, TaCIPKs ) including 55 new and 47 updated TaCIPKs in wheat. Phylogenetic analysis revealed that 123 TaCIPKs could be divided into four groups. Segmental duplications and tandem repeats promoted the expansion of the TaCIPK family. Gene function was further evidenced by differences in gene structure, cis -elements, and protein domains . TaCIPK15-4A was cloned in this study. TaCIPK15-4A contained 17 serine , seven tyrosine , and 15 threonine phosphorylation sites and localized in the plasma membrane and cytoplasm. TaCIPK15-4A expression was induced after Bgt inoculation. Virus-induced gene silencing and overexpression experiments indicated that TaCIPK15-4A could play a positive role in wheat disease resistance to Bgt . Overall, these results provide insights into the role of the TaCIPK gene family in wheat resistance and could be beneficial for further research to prevent Bgt infection.
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Claims 13
Claim · model-written summary
Genome screening identified 27 CIPK subfamilies and 123 transcripts in wheat.
- Taxon
- wheat
Triticum aestivumt0 - Outcome
- CIPK subfamiliese0
- Value
- 27e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified 27 CIPK subfamilies (123 CIPK transcripts, TaCIPKs ) including 55 new and 47 updated TaCIPKs in wheat”
t0“wheat”
Claim · model-written summary
The screening reported 55 new TaCIPKs.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- newe0
- Value
- 55e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including 55 new and 47 updated TaCIPKs in wheat”
t0“wheat”
Claim · model-written summary
The screening updated 47 TaCIPKs.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- updated TaCIPKse0
- Value
- 47e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including 55 new and 47 updated TaCIPKs in wheat”
t0“wheat”
Claim · model-written summary
Phylogenetic analysis divided 123 TaCIPKs into four groups.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- TaCIPKse0
- Value
- four groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“123 TaCIPKs could be divided into four groups”
t0“wheat”
Claim · model-written summary
Segmental duplications and tandem repeats promoted TaCIPK family expansion.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- expansion of the TaCIPK familye0
- Qualifier
- Segmental duplications and tandem repeatse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Segmental duplications and tandem repeats promoted the expansion of the TaCIPK family.”
t0“wheat”
Claim · model-written summary
Gene structure, cis-elements and protein domains differentiated gene function.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- Gene functione0
- Qualifier
- differences in gene structure, cis -elements, and protein domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene function was further evidenced by differences in gene structure, cis -elements, and protein domains”
t0“wheat”
Claim · model-written summary
TaCIPK15-4A was cloned.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCIPK15-4Ae0
- Outcome
- TaCIPK15-4A was clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK15-4A was cloned in this study.”
t0“wheat”
Claim · model-written summary
TaCIPK15-4A contained 17 serine phosphorylation sites.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCIPK15-4Ae0
- Outcome
- serinee0
- Value
- 17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK15-4A contained 17 serine , seven tyrosine , and 15 threonine phosphorylation sites”
t0“wheat”
Claim · model-written summary
TaCIPK15-4A contained seven tyrosine phosphorylation sites.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCIPK15-4Ae0
- Outcome
- tyrosinee0
- Value
- sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK15-4A contained 17 serine , seven tyrosine , and 15 threonine phosphorylation sites”
t0“wheat”
Claim · model-written summary
TaCIPK15-4A contained 15 threonine phosphorylation sites.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCIPK15-4Ae0
- Outcome
- threonine phosphorylation sitese0
- Value
- 15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK15-4A contained 17 serine , seven tyrosine , and 15 threonine phosphorylation sites”
t0“wheat”
Claim · model-written summary
TaCIPK15-4A localized in the plasma membrane and cytoplasm.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- plasma membrane and cytoplasme0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“localized in the plasma membrane and cytoplasm”
t0“wheat”
Claim · model-written summary
Bgt inoculation induced TaCIPK15-4A expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Bgt inoculatione0
- Interactor
- Bgte0pathogen
- Gene
- TaCIPK15-4Ae0
- Outcome
- TaCIPK15-4A expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK15-4A expression was induced after Bgt inoculation.”
t0“wheat”
Claim · model-written summary
Silencing and overexpression indicated TaCIPK15-4A positively contributes to wheat resistance to Bgt.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Bgte0pathogen
- Gene
- TaCIPK15-4Ae0
- Outcome
- wheat disease resistance to Bgte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing and overexpression experiments indicated that TaCIPK15-4A could play a positive role in wheat disease resistance to Bgt”
t0“wheat”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
- no effect
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.