Abstract
Anthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resistance via anthocyanin accumulation, and its potential molecular mechanism is still unclear. Therefore, it is of great significance for the normal growth and development of maize seedlings to explore the potential molecular mechanism of anthocyanin improving salt tolerance of seedlings via transcriptome analysis. In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions. In order to identify the specific genes involved in seedlings’ salt stress response, we generated two recombinant inbred lines (RILpur–W22 and RILbro–W22) by crossing pur–W22 and bro–W22, and then performed transcriptome analysis on seedlings grown under both non–treatment and salt treatment conditions. A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RILpur–W22 and RILbro–W22 seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups. Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation. Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated. These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane. Notably, the anthocyanin synthesis related genes in RILpur–W22 were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings. In summary, the results of the transcriptome analysis not only expanded our understanding of the complex molecular mechanism of anthocyanin in improving the salt tolerance of maize seedlings, but also, the DEGs specifically expressed in the salt–tolerant line (RILpur–W22) provided candidate genes for further genetic analysis.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Salt treatment yielded 6100 DEGs in RILpur–W22 seedlings relative to non-treated groups.
- Material
- RILpur–W22e2
- Outcome
- differentially expressed genes (DEGs)e2
- Value
- 6100e2
- Qualifier
- under salt–stressed conditions when compared to the non–treated groupse2
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e2“A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RILpur–W22 and RILbro–W22 seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups”
Claim · model-written summary
Salt treatment yielded 5710 DEGs in RILbro–W22 seedlings relative to non-treated groups.
- Material
- RILbro–W22e2
- Outcome
- differentially expressed genes (DEGs)e2
- Value
- 5710e2
- Qualifier
- under salt–stressed conditions when compared to the non–treated groupse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e2“A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RILpur–W22 and RILbro–W22 seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups”
Claim · model-written summary
3160 DEGs were present in both RILpur–W22 and RILbro–W22 under salt stress and were treated as commonly stressed DEGs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e3“Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation”
Claim · model-written summary
The commonly stressed DEGs were mainly enriched in the redox process, the monomer metabolic process and catalytic activity.
- Outcome
- the redox process, the monomer metabolic process, catalytic activitye3
- Qualifier
- mainly enriched ine3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e3“Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation”
Claim · model-written summary
The commonly stressed DEGs were mainly enriched in the plasma membrane and metabolic process regulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e3“Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation”
Claim · model-written summary
RILpur–W22 had 1728 specific DEGs not detected in the salt-sensitive RILbro–W22 line.
- Material
- RILpur–W22e4
- Outcome
- specific DEGse4
- Value
- 1728e4
- Qualifier
- not detected in the salt–sensitive RILbro–W22 linee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e4“Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated”
Claim · model-written summary
Of the specific DEGs in RILpur–W22, 887 were upregulated.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e4“Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated”
Claim · model-written summary
Of the specific DEGs in RILpur–W22, 841 were downregulated.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e4“Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated”
Claim · model-written summary
The DEGs were primarily associated with redox processes, biological regulation and the plasma membrane.
- Outcome
- These DEGse5
- Qualifier
- primarily associated with redox processes, biological regulation, and the plasma membranee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e5“These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane”
Claim · model-written summary
Anthocyanin synthesis related genes were strongly induced under salt treatment in RILpur–W22, consistent with its salt tolerance phenotype.
- Material
- RILpur–W22e6
- Treatment
- salt treatment conditionse6
- Gene
- anthocyanin synthesis related genese6
- Outcome
- anthocyanin synthesis related genese6
- Direction
- strongly inducede6
- Qualifier
- consistented with the salt tolerance phenotype of its seedlingse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e6“Notably, the anthocyanin synthesis related genes in RILpur–W22 were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings”
Claim · model-written summary
The specifically expressed DEGs of the salt-tolerant line provide candidate genes for further genetic analysis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e7“the DEGs specifically expressed in the salt–tolerant line (RILpur–W22) provided candidate genes for further genetic analysis”
Claim · model-written summary
Tolerant pur–W22 and sensitive bro–W22 lines did not differ in seedling characteristics without salt treatment.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salt Tolerance in Maize Seedlings”
e1“In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions”
The same questions in other papers
salinity · molecular: expression · Zea mays (maize)Positive and negative results
21 papers in the same direction as this paper · 10 in the opposite direction
- negative
- negative
- negative
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.