Abstract
The phytohormones strigolactones crosstalk with abscisic acid (ABA) in acclimation to osmotic stress, as ascertained in leaves. However, our knowledge about underground tissues is limited, and lacking in Arabidopsis: whether strigolactones affect ABA transport across plasma membranes has never been addressed. We evaluated the effect of strigolactones on the localization of ATP BINDING CASSETTE G25 (ABCG25), an ABA exporter in Arabidopsis thaliana. Wild-type, strigolactone-insensitive, and strigolactone-depleted seedlings expressing a green fluorescent protein:ABCG25 construct were treated with ABA or strigolactones, and green fluorescent protein was quantified by confocal microscopy in different subcellular compartments of epidermal root cells. We show that strigolactones promote the localization of an ABA transporter at the plasma membrane by enhancing its endosomal recycling. Genotypes altered in strigolactone synthesis or perception are not impaired in ABCG25 recycling promotion by ABA, which acts downstream or independent of strigolactones in this respect. Additionally, we confirm that osmotic stress decreases strigolactone synthesis in A. thaliana root cells, and that this decrease may support local ABA retention under low water availability by allowing ABCG25 internalization. Thus, we propose a new mechanism for ABA homeostasis regulation in the context of osmotic stress acclimation: the fine-tuning by strigolactones of ABCG25 localization in root cells.
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Claims 5
Claim · model-written summary
Strigolactones promote localization of an ABA transporter at the plasma membrane of root epidermal cells
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- root epidermal cellst0
- Treatment
- strigolactonese1
- Gene
- ABCG25t1
- Outcome
- localization of an ABA transporter at the plasma membranee1
- Direction
- promotee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“root epidermal cells of Arabidopsis thaliana”
t1“the ABA exporter ABCG25”
e1“We show that strigolactones promote the localization of an ABA transporter at the plasma membrane by enhancing its endosomal recycling”
Claim · model-written summary
Strigolactones promote ABCG25 localization by enhancing endosomal recycling
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- strigolactonese1
- Outcome
- endosomal recyclinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“root epidermal cells of Arabidopsis thaliana”
e1“We show that strigolactones promote the localization of an ABA transporter at the plasma membrane by enhancing its endosomal recycling”
Claim · model-written summary
Genotypes altered in strigolactone synthesis or perception are not impaired in ABCG25 recycling promotion by ABA
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Genotypes altered in strigolactone synthesis or perceptione2
- Treatment
- ABAe2
- Outcome
- ABCG25 recycling promotion by ABAe2
- Direction
- not impairede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“root epidermal cells of Arabidopsis thaliana”
e2“Genotypes altered in strigolactone synthesis or perception are not impaired in ABCG25 recycling promotion by ABA, which acts downstream or independent of strigolactones in this respect”
Claim · model-written summary
Osmotic stress decreases strigolactone synthesis in Arabidopsis root cells
- Taxon
- A. thalianaArabidopsis thalianae3
- Organ / stage
- root cellse3
- Treatment
- osmotic stresse3
- Outcome
- strigolactone synthesise3
- Direction
- decreasese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“we confirm that osmotic stress decreases strigolactone synthesis in A. thaliana root cells”
Claim · model-written summary
Strigolactone-regulated ABCG25 localization is proposed as a mechanism for ABA homeostasis under osmotic stress
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ABCG25e4
- Outcome
- ABA homeostasis regulatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“root epidermal cells of Arabidopsis thaliana”
e4“we propose a new mechanism for ABA homeostasis regulation in the context of osmotic stress acclimation: the fine-tuning by strigolactones of ABCG25 localization in root cells”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Arabidopsis thaliana (thale cress)Positive and negative results
7 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
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12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.