Abstract
An in vitro grafting method adapting the usual “indexing by green grafting” technique (Walter et al., 1990) to in vitro culture conditions was tested. The local grapevine varieties Khamri Tozeur, Akhel Meguergueb, Jerbi Degueche, Asli and Jebbi, infected respectively with infectious degeneration, leafroll, vein mosaic, corky bark and vein necrosis diseases, were used. Virus expression was greater on media having a greater number of nutrients such as the Van Hoof (1974) medium containing 12 macronutrients. On the other hand, the addition of BAP (0.25 mg l-1) to the medium reduced external virus symptoms on newly sprouted axillary shoots. When these shoots were transferred to fresh culture medium supplemented with IBA (0.1 mg l-1), typical and specific symptoms of major virus diseases clearly developed. Re-grafting of axillary shoots on the fragment of an infested clone can be used to overcome difficulties related to corky bark and vein mosaic symptom expression. We also demonstrated that viruses occur in general in mixed infections. The symptoms of a given virus become evident only when favourable conditions to it arise. Our research is still working on reducing the time of detecting virus and virus-like diseases. This is essential for sanitary selection of grapevine plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Virus expression was greater on nutrient richer medium.
- Taxon
- grapet0
- Treatment
- media having a greater number of nutrientse0
- Outcome
- Virus expressione0
- Direction
- greatere0
- Value
- 12 macronutrientse0
Not reportedComparator
Verbatim
e0“Virus expression was greater on media having a greater number of nutrients such as the Van Hoof (1974) medium containing 12 macronutrients”
t0“grape”
Claim · model-written summary
BAP supplementation reduced external virus symptoms on newly sprouted axillary shoots.
- Taxon
- grapet0
- Organ / stage
- newly sprouted axillary shootse0
- Treatment
- BAP (0.25 mg l-1)e0
- Outcome
- external virus symptomse0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“the addition of BAP (0.25 mg l-1) to the medium reduced external virus symptoms on newly sprouted axillary shoots”
t0“grape”
Claim · model-written summary
On IBA supplemented fresh medium, typical disease symptoms developed on transferred shoots.
- Taxon
- grapet0
- Organ / stage
- shootse0
- Treatment
- IBA (0.1 mg l-1)e0
- Outcome
- typical and specific symptoms of major virus diseasese0
Not reportedComparatorDirection
Verbatim
e0“these shoots were transferred to fresh culture medium supplemented with IBA (0.1 mg l-1), typical and specific symptoms of major virus diseases clearly developed”
t0“grape”
Claim · model-written summary
Regrafting axillary shoots on an infested clone helped reveal corky bark and vein mosaic symptoms.
- Taxon
- grapet0
- Treatment
- Re-grafting of axillary shoots on the fragment of an infested clonee0
- Outcome
- corky bark and vein mosaic symptom expressione0
Not reportedComparator
Verbatim
e0“Re-grafting of axillary shoots on the fragment of an infested clone can be used to overcome difficulties related to corky bark and vein mosaic symptom expression”
t0“grape”
Claim · model-written summary
Viruses generally occurred in mixed infections.
Verbatim
e0“viruses occur in general in mixed infections”
t0“grape”
The same questions in other papers
N-benzyladenine · disease · VitisResults agree
2 papers in the same direction as this paper
- positive
- positive
in vitro · molecular: expression · VitisPositive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.