Abstract
The high frequency, stable somatic embryo system of tea has still not been established due to the limitations of its own characteristics and therefore severely restricts the genetic research and breeding process of tea plants. In this study, the transcriptome was used to illustrate the mechanisms of gene expression regulation in the somatic embryogenesis of tea plants. The number of DEGs for the (IS intermediate stage)_PS (preliminary stage), ES (embryoid stage)_IS and ES_PS stages were 109, 2848 and 1697, respectively. The enrichment analysis showed that carbohydrate metabolic processes were considerably enriched at the ES_IS stage and performed a key role in somatic embryogenesis, while enhanced light capture in photosystem I could provide the material basis for carbohydrates. The pathway analysis showed that the enriched pathways in IS_PS process were far less than those in ES_IS or ES_PS, and the photosynthesis and photosynthetic antenna protein pathway of DEGs in ES_IS or ES_PS stage were notably enriched and up-regulated. The key photosynthesis and photosynthesis antenna protein pathways and the Lhcb1 gene were discovered in tea plants somatic embryogenesis. These results were of great significance to clarify the mechanism of somatic embryogenesis and the breeding research of tea plants.
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Claims 6
Claim · model-written summary
Differentially expressed genes numbered 109, 2848 and 1697 for the IS_PS, ES_IS and ES_PS comparisons respectively.
Verbatim
e0“The number of DEGs for the (IS intermediate stage)_PS (preliminary stage), ES (embryoid stage)_IS and ES_PS stages were 109, 2848 and 1697, respectively.”
e1“somatic embryogenesis of tea plants”
Claim · model-written summary
Carbohydrate metabolic processes were enriched at the ES_IS stage.
Verbatim
e0“carbohydrate metabolic processes were considerably enriched at the ES_IS stage”
e1“somatic embryogenesis of tea plants”
Claim · model-written summary
The authors infer that enhanced photosystem I light capture may supply carbohydrates for somatic embryogenesis.
Verbatim
e0“enhanced light capture in photosystem I could provide the material basis for carbohydrates”
e1“somatic embryogenesis of tea plants”
Claim · model-written summary
Fewer pathways were enriched in IS_PS than ES_IS or ES_PS.
Verbatim
e0“the enriched pathways in IS_PS process were far less than those in ES_IS or ES_PS”
e1“somatic embryogenesis of tea plants”
Claim · model-written summary
Photosynthesis and photosynthetic antenna protein pathways were enriched and up regulated in ES_IS or ES_PS stages.
Verbatim
e0“the photosynthesis and photosynthetic antenna protein pathway of DEGs in ES_IS or ES_PS stage were notably enriched and up-regulated”
e1“somatic embryogenesis of tea plants”
Claim · model-written summary
The Lhcb1 gene was identified in association with tea somatic embryogenesis.
Verbatim
e0“the Lhcb1 gene were discovered in tea plants somatic embryogenesis”