Paper · 2022 · Journal of Emerging Investigators

Biofortification of Raphanus sativus through irrigation with Ca2+ solutions does not increase calcium content

Grace Johnson; Ryne Emerick

3
claims
6
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Micronutrient deficiencies, which affect more than two billion people globally, occur when an individual does not receive adequate amounts of essential vitamins or minerals. Biofortification aims to increase the nutritional content of food crops, and it is an important tool in decreasing the effects of micronutrient deficiencies. Specifically, hypocalcemia, or calcium deficiency, causes a wide range of symptoms that affect many different body systems. In this study, we tested the hypothesis that increasing the amount of calcium available to the food crop during growth would increase the amount of calcium present in the food crop. A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations. The height of each plant was measured every five days beginning at day 0 to track the growth. At maturity, a random sample of four plants from each group were tested for calcium and magnesium content using an atomic absorption spectrophotometer. The group that received the highest concentration of Ca2+ grew significantly smaller than most other groups. The Ca2+ contents of leaf samples had no significant difference in Ca2+ or Mg2+ content; however, the Ca2+ content of root samples showed a significant decrease from the control in both Ca2+ and Mg2+ content across several experimental groups. These results provide preliminary evidence that irrigation with Ca2+ solutions does not increase the calcium content of mature plants, but further testing is needed to confirm these results. If increased calcium in irrigation water significantly increases the calcium content of mature radish plants, this technique could be used to increase the calcium content of other crops in areas with high rates of hypocalcemia.

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Claims 3

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

The highest Ca2+ irrigation concentration made radish plants grow significantly smaller than most other groups.

Treatment
highest concentration of Ca2+e2
Outcome
grewe2
Direction
smallere2
Comparator
most other groupse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e1

“A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations”

✓ located verbatim in the source · abstract, characters 638–780 · show in text ↑
e2

“The group that received the highest concentration of Ca2+ grew significantly smaller than most other groups”

✓ located verbatim in the source · abstract, characters 1028–1135 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Ca2+ irrigation did not change Ca2+ or Mg2+ content of radish leaf samples.

Organ / stage
leaf samplese3
Treatment
irrigated with water of varying Ca2+ concentrationse1
Outcome
Ca2+ contents of leaf samplese3
Direction
no significant differencee3
Qualifier
Ca2+ or Mg2+ contente3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e1

“A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations”

✓ located verbatim in the source · abstract, characters 638–780 · show in text ↑
e3

“The Ca2+ contents of leaf samples had no significant difference in Ca2+ or Mg2+ content”

✓ located verbatim in the source · abstract, characters 1137–1224 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Ca2+ irrigation significantly decreased Ca2+ and Mg2+ content of radish root samples relative to the control.

Organ / stage
root samplese4
Treatment
irrigated with water of varying Ca2+ concentrationse1
Outcome
Ca2+ and Mg2+ contente4
Direction
decreasee4
Comparator
the controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e1

“A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations”

✓ located verbatim in the source · abstract, characters 638–780 · show in text ↑
e4

“the Ca2+ content of root samples showed a significant decrease from the control in both Ca2+ and Mg2+ content across several experimental groups”

✓ located verbatim in the source · abstract, characters 1235–1379 · show in text ↑

calcium(2+) · nutrient: content · Raphanus sativus (radish)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

calcium(2+) · molecular: metabolites · Raphanus sativus (radish)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

irrigation · nutrient: content · Raphanus sativus (radish)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.