Abstract
Micronutrient deficiencies, which affect more than two billion people globally, occur when an individual does not receive adequate amounts of essential vitamins or minerals. Biofortification aims to increase the nutritional content of food crops, and it is an important tool in decreasing the effects of micronutrient deficiencies. Specifically, hypocalcemia, or calcium deficiency, causes a wide range of symptoms that affect many different body systems. In this study, we tested the hypothesis that increasing the amount of calcium available to the food crop during growth would increase the amount of calcium present in the food crop. A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations. The height of each plant was measured every five days beginning at day 0 to track the growth. At maturity, a random sample of four plants from each group were tested for calcium and magnesium content using an atomic absorption spectrophotometer. The group that received the highest concentration of Ca2+ grew significantly smaller than most other groups. The Ca2+ contents of leaf samples had no significant difference in Ca2+ or Mg2+ content; however, the Ca2+ content of root samples showed a significant decrease from the control in both Ca2+ and Mg2+ content across several experimental groups. These results provide preliminary evidence that irrigation with Ca2+ solutions does not increase the calcium content of mature plants, but further testing is needed to confirm these results. If increased calcium in irrigation water significantly increases the calcium content of mature radish plants, this technique could be used to increase the calcium content of other crops in areas with high rates of hypocalcemia.
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Claims 3
Claim · model-written summary
The highest Ca2+ irrigation concentration made radish plants grow significantly smaller than most other groups.
- Taxon
- radish
Raphanus sativuse1 - Treatment
- highest concentration of Ca2+e2
- Outcome
- grewe2
- Direction
- smallere2
- Comparator
- most other groupse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations”
e2“The group that received the highest concentration of Ca2+ grew significantly smaller than most other groups”
Claim · model-written summary
Ca2+ irrigation did not change Ca2+ or Mg2+ content of radish leaf samples.
- Taxon
- radish
Raphanus sativuse1 - Organ / stage
- leaf samplese3
- Treatment
- irrigated with water of varying Ca2+ concentrationse1
- Outcome
- Ca2+ contents of leaf samplese3
- Direction
- no significant differencee3
- Qualifier
- Ca2+ or Mg2+ contente3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations”
e3“The Ca2+ contents of leaf samples had no significant difference in Ca2+ or Mg2+ content”
Claim · model-written summary
Ca2+ irrigation significantly decreased Ca2+ and Mg2+ content of radish root samples relative to the control.
- Taxon
- radish
Raphanus sativuse1 - Organ / stage
- root samplese4
- Treatment
- irrigated with water of varying Ca2+ concentrationse1
- Outcome
- Ca2+ and Mg2+ contente4
- Direction
- decreasee4
- Comparator
- the controle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“A total of 180 radish (Raphanus sativus) plants were grown in a controlled environment and irrigated with water of varying Ca2+ concentrations”
e4“the Ca2+ content of root samples showed a significant decrease from the control in both Ca2+ and Mg2+ content across several experimental groups”
The same questions in other papers
calcium(2+) · nutrient: content · Raphanus sativus (radish)Positive and negative results
1 in the opposite direction
- positive
calcium(2+) · molecular: metabolites · Raphanus sativus (radish)Positive and negative results
1 in the opposite direction
- positive
irrigation · nutrient: content · Raphanus sativus (radish)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.