Abstract
MYB transcription factors play important roles during abiotic stress responses in plants. However, little is known about the accurate systematic analysis of MYB genes in the four cotton species, Gossypium hirsutum, G. barbadense, G. arboreum and G. raimondii. Herein, we performed phylogenetic analysis and showed that cotton MYBs and Arabidopsis MYBs were clustered in the same subfamilies for each species. The identified cotton MYBs were distributed unevenly on chromosomes in various densities for each species, wherein genome-wide tandem and segment duplications were the main driving force of MYB family expansion. Synteny analysis suggested that the abundant collinearity pairs of MYBs were identified between G. hirsutum and the other three species, and that they might have undergone strong purification selection. Characteristics of conserved motifs, along with their consensus sequence, promoter cis elements and gene structure, revealed that MYB proteins might be highly conserved in the same subgroups for each species. Subsequent analysis of differentially expressed genes and expression patterns indicated that most GhMYBs might be involved in response to drought (especially) and salt stress, which was supported by the expression levels of nine GhMYBs using real-time quantitative PCR. Finally, we performed a workflow that combined virus-induced gene silencing and the heterologous transformation of Arabidopsis, which confirmed the positive roles of GhMYBs under drought conditions, as validated by determining the drought-tolerant phenotypes, damage index and/or water loss rate. Collectively, our findings not only expand our understanding of the relationships between evolution and function of MYB genes, but they also provide candidate genes for cotton breeding.
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Claims 8
Claim · model-written summary
Cotton and Arabidopsis MYBs clustered in the same subfamilies.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- MYBse0
- Outcome
- clustered in the same subfamiliese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton MYBs and Arabidopsis MYBs were clustered in the same subfamilies for each species”
Claim · model-written summary
Cotton MYBs were unevenly distributed among chromosomes.
- Taxon
- cottonGossypium hirsutume0
- Gene
- MYBse0
- Outcome
- distributed unevenly on chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The identified cotton MYBs were distributed unevenly on chromosomes in various densities for each species”
Claim · model-written summary
Tandem and segment duplications drove cotton MYB family expansion.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- MYBe0
- Outcome
- MYB family expansione0
- Qualifier
- genome-wide tandem and segment duplicationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genome-wide tandem and segment duplications were the main driving force of MYB family expansion”
t0“Cotton MYB”
Claim · model-written summary
Abundant MYB collinearity pairs occurred between G. hirsutum and the other three cotton species.
- Taxon
- G. hirsutumGossypium hirsutume0
- Gene
- MYBse0
- Outcome
- abundant collinearity pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“abundant collinearity pairs of MYBs were identified between G. hirsutum and the other three species”
Claim · model-written summary
Cotton MYBs may have undergone strong purification selection.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- MYBt0
- Outcome
- strong purification selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they might have undergone strong purification selection”
t0“Cotton MYB”
Claim · model-written summary
MYB proteins may be highly conserved within the same cotton subgroups.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- MYB proteinse0
- Outcome
- highly conserved in the same subgroupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB proteins might be highly conserved in the same subgroups for each species”
t0“Cotton MYB”
Claim · model-written summary
Most GhMYBs may participate in drought and salt stress responses.
Not reportedDirection
Verbatim
e0“most GhMYBs might be involved in response to drought (especially) and salt stress”
Claim · model-written summary
GhMYBs had positive roles under drought conditions, supported by drought tolerance, damage and water loss measures.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- virus-induced gene silencing and the heterologous transformation of Arabidopsise0
- Gene
- GhMYBse0
- Outcome
- drought-tolerant phenotypes, damage index and/or water loss ratee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced gene silencing and the heterologous transformation of Arabidopsis, which confirmed the positive roles of GhMYBs under drought conditions, as validated by determining the drought-tolerant phenotypes, damage index and/or water loss rate”
t0“Cotton MYB”
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- negative
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.