Abstract
Puccinia striiformis f. sp. tritici (Pst) secretes effector proteins that enter plant cells and manipulate host processes. In a previous study, we identified a glycine-serine-rich effector PstGSRE4, which was proven to regulate the reactive oxygen species (ROS) pathway by interacting with TaCZSOD2. In this study, we further demonstrated that PstGSRE4 interacts with wheat glyceraldehyde-3-phosphate dehydrogenase TaGAPDH2, which is related to ROS signalling. In wheat, silencing of TaGAPDH2 by virus-induced gene silencing increased the accumulation of ROS induced by the Pst virulent race CYR31. Overexpression of TaGAPDH2 decreased the accumulation of ROS induced by the avirulent Pst race CYR23. In addition, TaGAPDH2 suppressed Pst candidate elicitor Pst322-triggered cell death by decreasing ROS accumulation in Nicotiana benthamiana. Knocking down TaGAPDH2 expression attenuated Pst infection, whereas overexpression of TaGAPDH2 promoted Pst infection, indicating that TaGAPDH2 is a negative regulator of plant defence. In N. benthamiana, PstGSRE4 stabilized TaGAPDH2 through inhibition of the 26S proteasome-mediated destabilization. Overall, these results suggest that TaGAPDH2 is hijacked by the Pst effector as a negative regulator of plant immunity to promote Pst infection in wheat.
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Claims 9
Claim · model-written summary
Silencing of TaGAPDH2 in wheat increased ROS accumulation induced by the virulent Pst race CYR31.
- Taxon
- wheat
Triticum aestivume0 - Material
- virulent race CYR31e0
- Treatment
- silencing of TaGAPDH2 by virus-induced gene silencinge0
- Interactor
- Pste0pathogen
- Gene
- TaGAPDH2e0
- Outcome
- accumulation of ROSe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In wheat, silencing of TaGAPDH2 by virus-induced gene silencing increased the accumulation of ROS induced by the Pst virulent race CYR31”
Claim · model-written summary
Overexpression of TaGAPDH2 in wheat decreased ROS accumulation induced by the avirulent Pst race CYR23.
- Taxon
- wheatTriticum aestivume1
- Material
- avirulent Pst race CYR23e0
- Treatment
- Overexpression of TaGAPDH2e0
- Interactor
- Pste0pathogen
- Gene
- TaGAPDH2e0
- Outcome
- accumulation of ROSe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaGAPDH2 decreased the accumulation of ROS induced by the avirulent Pst race CYR23”
e1“to promote Pst infection in wheat”
Claim · model-written summary
TaGAPDH2 suppressed the cell death triggered by the Pst candidate elicitor Pst322 in Nicotiana benthamiana by decreasing ROS accumulation.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- TaGAPDH2e0
- Interactor
- Pste0pathogen
- Gene
- TaGAPDH2e0
- Outcome
- cell deathe0
- Direction
- suppressede0
- Qualifier
- by decreasing ROS accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaGAPDH2 suppressed Pst candidate elicitor Pst322-triggered cell death by decreasing ROS accumulation in Nicotiana benthamiana”
Claim · model-written summary
Knocking down TaGAPDH2 in wheat attenuated Pst infection.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knocking down TaGAPDH2 expressione0
- Interactor
- Pste0pathogen
- Gene
- TaGAPDH2e0
- Outcome
- Pst infectione0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knocking down TaGAPDH2 expression attenuated Pst infection, whereas overexpression of TaGAPDH2 promoted Pst infection”
e1“to promote Pst infection in wheat”
Claim · model-written summary
Overexpression of TaGAPDH2 in wheat promoted Pst infection.
- Taxon
- wheatTriticum aestivume1
- Treatment
- overexpression of TaGAPDH2e0
- Interactor
- Pste0pathogen
- Gene
- TaGAPDH2e0
- Outcome
- Pst infectione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knocking down TaGAPDH2 expression attenuated Pst infection, whereas overexpression of TaGAPDH2 promoted Pst infection”
e1“to promote Pst infection in wheat”
Claim · model-written summary
PstGSRE4 stabilized TaGAPDH2 in Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- PstGSRE4e0
- Gene
- TaGAPDH2e0
- Outcome
- TaGAPDH2e0
- Direction
- stabilizede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, PstGSRE4 stabilized TaGAPDH2 through inhibition of the 26S proteasome-mediated destabilization”
Claim · model-written summary
PstGSRE4 stabilizes TaGAPDH2 through inhibition of the 26S proteasome-mediated destabilization.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Outcome
- 26S proteasome-mediated destabilizatione0
- Qualifier
- inhibition of the 26S proteasomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, PstGSRE4 stabilized TaGAPDH2 through inhibition of the 26S proteasome-mediated destabilization”
Claim · model-written summary
PstGSRE4 interacts with wheat TaGAPDH2.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaGAPDH2e1
- Outcome
- interacts withe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PstGSRE4 interacts with wheat”
e1“dehydrogenase TaGAPDH2, which is related to ROS signalling”
Claim · model-written summary
TaGAPDH2 is hijacked by the Pst effector as a negative regulator of plant immunity that promotes Pst infection in wheat.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pste0pathogen
- Gene
- TaGAPDH2e0
- Outcome
- plant immunitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaGAPDH2 is hijacked by the Pst effector as a negative regulator of plant immunity to promote Pst infection in wheat”
The same questions in other papers
loss of function · physiology: oxidative · Triticum aestivum (wheat)Positive and negative results
5 papers in the same direction as this paper · 17 in the opposite direction
- positive
- negative
- negative
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.