Abstract
SUMMARY Cell polarity is the foundation of cell development and tissue morphogenesis. The investigation of polarized growth provides opportunities to gain profound insights into morphogenesis and tissue functionality in organisms. Currently, there are still many mysteries surrounding the mechanisms that regulate polarized cell growth. Cotton fiber cells serve as an excellent model for studying polarized growth, and provide important clues for unraveling the molecular mechanisms, signaling pathways, and regulatory networks of polarized growth. In this study, we characterized two functional genes, GhMDHAR1A T /D T and GhDHAR2A T /D T with predominant expression during fiber elongation. Loss of function of both genes contributed to a significant increase in fiber length. Transcriptomic data revealed up‐regulated expression of antioxidant genes in CRISPR mutant lines, along with delayed expression of secondary wall‐related genes and temporally prolonged expression of primary wall‐related genes. Experimental evidence demonstrated that the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T‐AOC), resulting in reduced H 2 O 2 levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines. Moreover, the increased polysaccharide synthesis in CRISPR mutant lines was found to provide an abundant supply of raw materials for fiber cell wall elongation, suggesting that synergistic interplay between redox homeostasis and polysaccharide synthesis in fiber cells may facilitate cell wall remodeling and fiber elongation. This study provides valuable insights for deciphering the mechanisms of cell polarized growth and improving cotton fiber quality.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Loss of function of GhMDHAR1A and GhDHAR2A significantly increased cotton fiber length.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- Loss of function of both genese1
- Gene
- GhMDHAR1Ae0
- Outcome
- fiber lengthe1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e0“we characterized two functional genes, GhMDHAR1A T /D T and GhDHAR2A T /D T with predominant expression during fiber elongation”
e1“Loss of function of both genes contributed to a significant increase in fiber length”
Claim · model-written summary
CRISPR mutant lines showed up-regulated expression of antioxidant genes.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese2
- Outcome
- expression of antioxidant genese2
- Direction
- up‐regulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e2“Transcriptomic data revealed up‐regulated expression of antioxidant genes in CRISPR mutant lines, along with delayed expression of secondary wall‐related genes and temporally prolonged expression of primary wall‐related genes”
Claim · model-written summary
CRISPR mutant lines showed delayed expression of secondary wall-related genes.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese2
- Outcome
- expression of secondary wall‐related genese2
- Direction
- delayede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e2“Transcriptomic data revealed up‐regulated expression of antioxidant genes in CRISPR mutant lines, along with delayed expression of secondary wall‐related genes and temporally prolonged expression of primary wall‐related genes”
Claim · model-written summary
CRISPR mutant lines showed temporally prolonged expression of primary wall-related genes.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese2
- Outcome
- expression of primary wall‐related genese2
- Direction
- temporally prolongede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e2“Transcriptomic data revealed up‐regulated expression of antioxidant genes in CRISPR mutant lines, along with delayed expression of secondary wall‐related genes and temporally prolonged expression of primary wall‐related genes”
Claim · model-written summary
GSH content increased in CRISPR mutant lines.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese3
- Outcome
- GSH contente3
- Direction
- increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e3“the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T‐AOC), resulting in reduced H 2 O 2 levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines”
Claim · model-written summary
Glutathione peroxidase (GPX) enzyme activity increased in CRISPR mutant lines.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese3
- Outcome
- glutathione peroxidase (GPX) enzyme activitye3
- Direction
- increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e3“the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T‐AOC), resulting in reduced H 2 O 2 levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines”
Claim · model-written summary
Total antioxidant capacity (T-AOC) was enhanced in CRISPR mutant lines.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese3
- Outcome
- total antioxidant capacity (T‐AOC)e3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e3“the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T‐AOC), resulting in reduced H 2 O 2 levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines”
Claim · model-written summary
H2O2 levels were reduced in CRISPR mutant lines.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese3
- Outcome
- H 2 O 2 levelse3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e3“the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T‐AOC), resulting in reduced H 2 O 2 levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines”
Claim · model-written summary
The reduced H2O2 levels are proposed to explain the extension of the fiber elongation stage in CRISPR mutant lines.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- extension of fiber elongation stagee3
- Qualifier
- reduced H 2 O 2 levelse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e3“the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T‐AOC), resulting in reduced H 2 O 2 levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines”
Claim · model-written summary
Polysaccharide synthesis increased in CRISPR mutant lines.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- CRISPR mutant linese4
- Outcome
- polysaccharide synthesise4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e4“the increased polysaccharide synthesis in CRISPR mutant lines was found to provide an abundant supply of raw materials for fiber cell wall elongation, suggesting that synergistic interplay between redox homeostasis and polysaccharide synthesis in fiber cells may facilitate cell wall remodeling and fiber elongation”
Claim · model-written summary
Increased polysaccharide synthesis is proposed to supply raw materials for fiber cell wall elongation.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- fiber cell wall elongatione4
- Qualifier
- an abundant supply of raw materialse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e4“the increased polysaccharide synthesis in CRISPR mutant lines was found to provide an abundant supply of raw materials for fiber cell wall elongation, suggesting that synergistic interplay between redox homeostasis and polysaccharide synthesis in fiber cells may facilitate cell wall remodeling and fiber elongation”
Claim · model-written summary
Synergistic interplay between redox homeostasis and polysaccharide synthesis may facilitate cell wall remodeling and fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- cell wall remodeling and fiber elongatione4
- Qualifier
- synergistic interplay between redox homeostasis and polysaccharide synthesise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“promotes cotton fiber elongation”
e4“the increased polysaccharide synthesis in CRISPR mutant lines was found to provide an abundant supply of raw materials for fiber cell wall elongation, suggesting that synergistic interplay between redox homeostasis and polysaccharide synthesis in fiber cells may facilitate cell wall remodeling and fiber elongation”
The same questions in other papers
loss of function · quality: grain · Gossypium hirsutum (cotton)Positive and negative results
10 papers in the same direction as this paper · 30 in the opposite direction
- negative
- negative
- positive
37 papers more in this group
See all 41 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.