Abstract
Abstract Avicennia marina is a salt-tolerance plant with high antioxidant and antibacterial potential. In the present work, a gene encoding MnSOD from Avicennia marina (AmSOD2) was cloned in the expression vectors pET28a. The resulting constructs were transformed into Escherichia coli strains Rosetta (DE3). Following the induction with Isopropyl β- d -1-thiogalactopyranoside, the protein His-AmSOD2 was expressed but dominantly found in the insoluble fraction of strain R-AmSOD2. Due to detection of mitochondrial transit peptide in the amino acid sequence of AmSOD2, the transit peptide was removed and AmSOD2 without transit peptide (tAmSOD2) was expressed in E. coli and dominantly found in the soluble fraction. The enzyme His-tAmSOD2 exhibited a molecular mass of 116 kDa in native condition. Nevertheless, in reducing conditions the molecular mass is 28 kDa indicating the enzyme His-tAmSOD2 is a tetramer protein. As shown by ICP analysis there is one mole Mn 2+ in each monomer. The Pure His-tAmSOD2 was highly active in vitro , however the activity was almost three-fold lower than His-AmSOD1. Whereas the high stability of the recombinant His-AmSOD1was previously shown after incubation in a broad range pH and high temperature, His-tAmSOD2 was stable up to 50 °C and pH 6 for 1 h. The gene expression analysis showed that the gene encoding AmSOD2 is expressed in root, shoot and leaves of A. marina . In addition, the results show that the expression in the leaves was enhanced after treatment of plant with NaCl, H 2 O 2 , Cd 2+ and Ni 2+ indicating the important role of MnSOD in the resistant mechanism of mangroves.
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Claims 5
Claim · model-written summary
The purified His-tAmSOD2 was highly active in vitro, but its activity was almost three-fold lower than that of His-AmSOD1.
- Taxon
- Avicennia marina
Avicennia marinae0 - Gene
- His-tAmSOD2e1
- Outcome
- activitye1
- Direction
- lowere1
- Comparator
- His-AmSOD1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avicennia marina is a salt-tolerance plant”
e1“The Pure His-tAmSOD2 was highly active in vitro , however the activity was almost three-fold lower than His-AmSOD1”
Claim · model-written summary
His-tAmSOD2 was stable up to 50 °C and pH 6 for 1 h.
- Taxon
- Avicennia marinaAvicennia marinae0
- Gene
- His-tAmSOD2e2
- Outcome
- stablee2
- Value
- 50 °Ce2
- Qualifier
- pH 6 for 1 he2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avicennia marina is a salt-tolerance plant”
e2“His-tAmSOD2 was stable up to 50 °C and pH 6 for 1 h”
Claim · model-written summary
His-tAmSOD2 has a native molecular mass of 116 kDa and a 28 kDa subunit mass under reducing conditions, indicating a tetramer.
- Taxon
- Avicennia marinaAvicennia marinae0
- Gene
- His-tAmSOD2e3
- Outcome
- molecular masse3
- Value
- 116 kDae3
- Qualifier
- in native conditione3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avicennia marina is a salt-tolerance plant”
e3“The enzyme His-tAmSOD2 exhibited a molecular mass of 116 kDa in native condition”
e4“in reducing conditions the molecular mass is 28 kDa”
Claim · model-written summary
AmSOD2 gene expression was detected in root, shoot and leaves of A. marina.
- Taxon
- A. marinaAvicennia marinae5
- Organ / stage
- root, shoot and leavese5
- Gene
- AmSOD2e5
- Outcome
- gene expressione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avicennia marina is a salt-tolerance plant”
e5“The gene expression analysis showed that the gene encoding AmSOD2 is expressed in root, shoot and leaves of A. marina”
Claim · model-written summary
Leaf expression of AmSOD2 was enhanced after treatment of plants with NaCl, H2O2, Cd2+ and Ni2+.
- Taxon
- Avicennia marinaAvicennia marinae0
- Organ / stage
- leavese6
- Treatment
- NaCl, H 2 O 2 , Cd 2+ and Ni 2+e6
- Outcome
- expression in the leavese6
- Direction
- enhancede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avicennia marina is a salt-tolerance plant”
e6“the expression in the leaves was enhanced after treatment of plant with NaCl, H 2 O 2 , Cd 2+ and Ni 2+”