Abstract
Geminiviruses are a group of single-stranded DNA viruses that have developed multiple strategies to overcome host defenses and establish viral infections. Sucrose nonfermenting-1-related kinase 1 (SnRK1) is a key regulator of energy balance in plants and plays an important role in plant development and immune defenses. As a heterotrimeric complex, SnRK1 is composed of a catalytic subunit α (SnRK1 α) and two regulatory subunits, β and γ. Previous studies on SnRK1 in plant defenses against microbial pathogens have mainly focused on SnRK1 α. In this study, we validated the interaction between the C4 protein encoded by tobacco leaf curl Yunnan virus (TbLCYnV) and the regulatory subunit β of Nicotiana benthamiana SnRK1, i.e., NbSnRK1 β2, and identified that the Asp22 of C4 is critical for TbLCYnV C4–NbSnRK1 β2 interactions. NbSnRK1 β2 silencing in N. benthamiana enhances susceptibility to TbLCYnV infection. Plants infected with viral mutant TbLCYnV (C4D22A), which contains the mutant version C4 (D22A) that is incapable of interacting with NbSnRK1 β2, display milder symptoms and lower viral accumulation. Furthermore, we discovered that C4 promotes NbSnRK1 β2 degradation via the autophagy pathway. We herein propose a model by which the geminivirus C4 protein causes NbSnRK1 β2 degradation via the TbLCYnV C4–NbSnRK1 β2 interaction to antagonize host antiviral defenses and facilitates viral infection and symptom development in N. benthamiana.
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Claims 5
Claim · model-written summary
Viral C4 interacted with host NbSnRK1 β2.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- tobacco leaf curl Yunnan virus (TbLCYnV)Tobacco leaf curl Yunnan viruse0pathogen
- Gene
- NbSnRK1 β2e0
- Outcome
- interaction between the C4 protein encoded by tobacco leaf curl Yunnan virus (TbLCYnV) and the regulatory subunit β of Nicotiana benthamiana SnRK1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we validated the interaction between the C4 protein encoded by tobacco leaf curl Yunnan virus (TbLCYnV) and the regulatory subunit β of Nicotiana benthamiana SnRK1, i.e., NbSnRK1 β2”
Claim · model-written summary
Silencing NbSnRK1 β2 increased plant susceptibility to viral infection.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- NbSnRK1 β2 silencinge0
- Interactor
- TbLCYnVe0pathogen
- Gene
- NbSnRK1 β2e0
- Outcome
- susceptibility to TbLCYnV infectione0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbSnRK1 β2 silencing in N. benthamiana enhances susceptibility to TbLCYnV infection.”
Claim · model-written summary
Plants infected with the viral mutant showed milder symptoms.
- Taxon
- PlantsViridiplantaee0
- Interactor
- TbLCYnVe0pathogen
- Outcome
- symptomse0
- Direction
- mildere0
Verbatim
e0“Plants infected with viral mutant TbLCYnV (C4D22A), which contains the mutant version C4 (D22A) that is incapable of interacting with NbSnRK1 β2, display milder symptoms and lower viral accumulation.”
Claim · model-written summary
Plants infected with the viral mutant showed lower viral accumulation.
- Taxon
- PlantsViridiplantaee0
- Interactor
- TbLCYnVe0pathogen
- Outcome
- viral accumulatione0
- Direction
- lowere0
Verbatim
e0“Plants infected with viral mutant TbLCYnV (C4D22A), which contains the mutant version C4 (D22A) that is incapable of interacting with NbSnRK1 β2, display milder symptoms and lower viral accumulation.”
Claim · model-written summary
Viral C4 promoted host NbSnRK1 β2 degradation through autophagy.
Not reportedTaxon
Verbatim
e0“C4 promotes NbSnRK1 β2 degradation via the autophagy pathway.”
The same questions in other papers
loss of function · disease · Nicotiana benthamianaPositive and negative results
92 papers in the same direction as this paper · 50 in the opposite direction
- negative
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