Abstract
Auxin response factors (ARFs) play a crucial role in regulating gene expression within the auxin signal transduction pathway, particularly during adventitious root (AR) formation. In this investigation, we identified full-length sequences for ARF17 and ARF18, encompassing 1,800 and 2,055 bp, encoding 599 and 684 amino acid residues, respectively. Despite exhibiting low sequence homology, the ARF17- and ARF18-encoded proteins displayed significant structural similarity and shared identical motifs. Phylogenetic analysis revealed close relationships between NnARF17 and VvARF17, as well as NnARF18 and BvARF18. Both ARF17 and ARF18 demonstrated responsiveness to exogenous indole-3-acetic acid (IAA), ethephon, and sucrose, exhibiting organ-specific expression patterns. Beyond their role in promoting root development, these ARFs enhanced stem growth and conferred drought tolerance while mitigating waterlogging stress in transgenic Arabidopsis plants. RNA sequencing data indicated upregulation of 51 and 75 genes in ARF17 and ARF18 transgenic plants, respectively, including five and three genes associated with hormone metabolism and responses. Further analysis of transgenic plants revealed a significant decrease in IAA content, accompanied by a marked increase in abscisic acid content under normal growth conditions. Additionally, lotus seedlings treated with IAA exhibited elevated levels of polyphenol oxidase, IAA oxidase, and peroxidase. The consistent modulation of IAA content in both lotus and transgenic plants highlights the pivotal role of IAA in AR formation in lotus seedlings.
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Claims 15
Claim · model-written summary
Lotus ARF17 was identified as a 1,800 bp sequence encoding 599 amino acids.
.jpg?width=60){kind=small}
LotusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“we identified full-length sequences for ARF17 and ARF18, encompassing 1,800 and 2,055 bp, encoding 599 and 684 amino acid residues, respectively”
t0“lotus”
Claim · model-written summary
Lotus ARF18 was identified as a 2,055 bp sequence encoding 684 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified full-length sequences for ARF17 and ARF18, encompassing 1,800 and 2,055 bp, encoding 599 and 684 amino acid residues, respectively”
t0“lotus”
Claim · model-written summary
ARF17 and ARF18 proteins shared structural similarity and identical motifs despite low sequence homology.
- Gene
- ARF17- and ARF18-encoded proteinse0
- Outcome
- significant structural similarity and shared identical motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Despite exhibiting low sequence homology, the ARF17- and ARF18-encoded proteins displayed significant structural similarity and shared identical motifs”
t0“lotus”
Claim · model-written summary
NnARF17 grouped closely with VvARF17.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis revealed close relationships between NnARF17 and VvARF17, as well as NnARF18 and BvARF18”
t0“lotus”
Claim · model-written summary
NnARF18 grouped closely with BvARF18.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis revealed close relationships between NnARF17 and VvARF17, as well as NnARF18 and BvARF18”
t0“lotus”
Claim · model-written summary
Both ARFs responded to IAA, ethephon and sucrose and showed organ-specific expression.
- Treatment
- exogenous indole-3-acetic acid (IAA), ethephon, and sucrosee0
- Gene
- ARF17 and ARF18e0
- Outcome
- organ-specific expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both ARF17 and ARF18 demonstrated responsiveness to exogenous indole-3-acetic acid (IAA), ethephon, and sucrose, exhibiting organ-specific expression patterns”
t0“lotus”
Claim · model-written summary
ARF transgenes promoted root development in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- these ARFse0
- Outcome
- root developmente0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Beyond their role in promoting root development, these ARFs enhanced stem growth and conferred drought tolerance while mitigating waterlogging stress in transgenic Arabidopsis plants”
Claim · model-written summary
ARF transgenes enhanced Arabidopsis stem growth.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- these ARFse0
- Outcome
- stem growthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these ARFs enhanced stem growth and conferred drought tolerance while mitigating waterlogging stress in transgenic Arabidopsis plants”
Claim · model-written summary
ARF transgenes conferred drought tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- these ARFse0
- Outcome
- drought tolerancee0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these ARFs enhanced stem growth and conferred drought tolerance while mitigating waterlogging stress in transgenic Arabidopsis plants”
Claim · model-written summary
ARF transgenes mitigated waterlogging stress in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- these ARFse0
- Outcome
- waterlogging stresse0
- Direction
- mitigatinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these ARFs enhanced stem growth and conferred drought tolerance while mitigating waterlogging stress in transgenic Arabidopsis plants”
Claim · model-written summary
ARF17 transgenic plants showed upregulation of 51 genes, including five hormone-related genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA sequencing data indicated upregulation of 51 and 75 genes in ARF17 and ARF18 transgenic plants, respectively, including five and three genes associated with hormone metabolism and responses”
t0“Arabidopsis thaliana”
Claim · model-written summary
ARF18 transgenic plants showed upregulation of 75 genes, including three hormone-related genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA sequencing data indicated upregulation of 51 and 75 genes in ARF17 and ARF18 transgenic plants, respectively, including five and three genes associated with hormone metabolism and responses”
t0“Arabidopsis thaliana”
Claim · model-written summary
Transgenic Arabidopsis had decreased IAA content under normal growth conditions.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- IAA contente0
- Direction
- decreasee0
- Qualifier
- normal growth conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants revealed a significant decrease in IAA content, accompanied by a marked increase in abscisic acid content under normal growth conditions”
t0“Arabidopsis thaliana”
Claim · model-written summary
Transgenic Arabidopsis had increased abscisic acid content under normal growth conditions.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- abscisic acid contente0
- Direction
- increasee0
- Qualifier
- normal growth conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants revealed a significant decrease in IAA content, accompanied by a marked increase in abscisic acid content under normal growth conditions”
t0“Arabidopsis thaliana”
Claim · model-written summary
IAA-treated lotus seedlings had elevated polyphenol oxidase, IAA oxidase and peroxidase levels.
- Organ / stage
- seedlingse0
- Treatment
- IAAe0
- Outcome
- levels of polyphenol oxidase, IAA oxidase, and peroxidasee0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lotus seedlings treated with IAA exhibited elevated levels of polyphenol oxidase, IAA oxidase, and peroxidase”
The same questions in other papers
class · growth · ArabidopsisPositive and negative results
41 papers in the same direction as this paper · 28 in the opposite direction
- negative
- negative
- no effect
66 papers more in this group
See all 70 papers →class · tolerance: abiotic · ArabidopsisResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.