Paper · 2024 · PLoS ONE

Nitrogen use efficiency in bread wheat: Genetic variation and prospects for improvement

Suma S. Biradar; Mahalaxmi K. Patil; S. A. Desai; et al.

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verbatim quotes
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citations
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Open access · cc-by

Abstract

Nitrogen (N) is one of the primary macronutrients required for crop growth and yield. This nutrient is especially limiting wheat yields in the dry and low fertile agro-ecologies having low N in the root zone soil strata. Moreover, majority of farmers in India and South Asia are small to marginal with meagre capacity to invest in costly nitrogen fertilizers. Therefore, there is an immense need to identify lines that use nitrogen efficiently. A set of 50 diverse wheat genotypes consisting of indigenous germplasm lines (05), cultivars released for commercial cultivation (23) and selected elite lines from CIMMYT nurseries (22) were evaluated in an alpha-lattice design with two replications, a six-rowed plot of 2.5m length for 24 agro morphological, physiological and NUE related traits during two consecutive crop seasons in an N-depleted precision field under two different N levels of 50%-N50 (T1) and 100%-N100 (T2) of recommended N, i.e., 100 kg/ha. Analysis of variance revealed significant genetic variation among genotypes for all the traits studied. About 11.36% yield reduction was observed at reduced N levels. Significant correlations among NUE traits and yield component traits were observed which indicated pivotal role of N remobilization to the grain in enhancing yield levels. Among N-insensitive genotypes identified based on their yielding ability at low N levels, UASBW13356, UASBW13358, UASBW13354, UASBW13357 and KRL1-4 showed their inherent genotypic plasticity toward N application. The genotypes with more yield and high to moderate NUtE can be used as parents for the breeding of N efficient genotypes for marginal agro-ecologies. Low N tolerant genotypes identified from the current investigation may be further utilized in the identification of genomic regions responsible for NUE and its deployment in wheat breeding programs. The comprehensive data of 24 traits under different nitrogen levels for diverse genotypes from India and global sources (mainly CIMMYT) should be useful for supporting breeding for NUE and thus will be of great help for small and marginal farmers in India and South Asia.

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Claims 6

Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Wheat genotypes varied significantly for all studied traits.

Outcome
genetic variation among genotypes for all the traits studiede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Analysis of variance revealed significant genetic variation among genotypes for all the traits studied”

✓ located verbatim in the source · abstract, characters 960–1062 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 33–38 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reduced nitrogen lowered yield by about 11.36%.

Treatment
reduced N levelse0
Outcome
yielde0
Direction
reductione0
Value
11.36%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“About 11.36% yield reduction was observed at reduced N levels”

✓ located verbatim in the source · abstract, characters 1064–1125 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 33–38 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

NUE traits and yield component traits were significantly correlated.

Outcome
correlations among NUE traits and yield component traitse0

Not reportedMaterialDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Significant correlations among NUE traits and yield component traits were observed”

✓ located verbatim in the source · abstract, characters 1127–1209 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 33–38 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Five genotypes were identified as N-insensitive based on yield at low N.

Material
UASBW13356, UASBW13358, UASBW13354, UASBW13357 and KRL1-4e0
Outcome
genotypic plasticity toward N applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among N-insensitive genotypes identified based on their yielding ability at low N levels, UASBW13356, UASBW13358, UASBW13354, UASBW13357 and KRL1-4 showed their inherent genotypic plasticity toward N application”

✓ located verbatim in the source · abstract, characters 1299–1510 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 33–38 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

High yielding genotypes with high to moderate NUtE were proposed as breeding parents.

Outcome
used as parents for the breeding of N efficient genotypese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The genotypes with more yield and high to moderate NUtE can be used as parents for the breeding of N efficient genotypes for marginal agro-ecologies”

✓ located verbatim in the source · abstract, characters 1512–1660 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 33–38 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Low N tolerant genotypes may be used to identify genomic regions for NUE.

Outcome
identification of genomic regions responsible for NUEe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Low N tolerant genotypes identified from the current investigation may be further utilized in the identification of genomic regions responsible for NUE and its deployment in wheat breeding programs”

✓ located verbatim in the source · abstract, characters 1662–1859 · show in text ↑

nitrogen · yield · Triticum aestivum (wheat)Positive and negative results

2,061 papers: 1,737 positive, 324 negative or no effect

323 papers in the same direction as this paper · 1,737 in the opposite direction

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  3. negative

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