Abstract
The objective of this study was to develop a simultaneous analytical method for the determination of lignans, tocols, phytosterols, and squalene using high-performance liquid chromatography coupled with a diode array and fluorescence detector (HPLC-DAD-FLD). The method employed a VertisepTM UPS silica HPLC column (4.6 × 250 mm, 5 µm) with a mobile phase mixture of n-hexane/tetrahydrofuran/2-propanol. This approach enabled the simultaneous analysis of ten compounds within 22 min. The linear correlation (R2) exceeded 0.9901. The limit of detection (LOD) and limit of quantitation (LOQ) were up to 0.43 µg mL−1 for lignans and tocopherols and up to 326.23 µg mL−1 for phytosterol and squalene. The precision and accuracy of the intra-day and inter-day variation were less than 1.09 and 3.32% relative standard deviations (RSDs). Furthermore, the developed method was applied for the analysis of targeted compounds in twenty-eight sesame oil samples (1775–8965 µg g−1 total lignans, 29.7–687.9 µg g−1 total tocopherols, 2640–9500 µg g−1 phytosterol, and 245–4030 µg g−1 squalene). The HPLC method that has been developed was proven to be a reliable and effective tool for the determination of those functional compounds among sesame oil samples.
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Claims 9
Claim · model-written summary
The HPLC method analyzed ten compounds within 22 minutes.
Verbatim
e0“This approach enabled the simultaneous analysis of ten compounds within 22 min”
Claim · model-written summary
Method linear correlation exceeded 0.9901.
Verbatim
e0“The linear correlation (R2) exceeded 0.9901”
Claim · model-written summary
Detection and quantitation limits for lignans and tocopherols were up to 0.43 µg mL−1.
Verbatim
e0“The limit of detection (LOD) and limit of quantitation (LOQ) were up to 0.43 µg mL−1 for lignans and tocopherols”
Claim · model-written summary
Detection and quantitation limits for phytosterol and squalene were up to 326.23 µg mL−1.
Verbatim
e0“The limit of detection (LOD) and limit of quantitation (LOQ) were up to 0.43 µg mL−1 for lignans and tocopherols and up to 326.23 µg mL−1 for phytosterol and squalene.”
Claim · model-written summary
Intra-day and inter-day precision and accuracy variations were less than 1.09 and 3.32% RSDs.
Verbatim
e0“The precision and accuracy of the intra-day and inter-day variation were less than 1.09 and 3.32% relative standard deviations (RSDs)”
Claim · model-written summary
Sesame oil samples contained 1775–8965 µg g−1 total lignans.
- Taxon
- sesame
Sesamum indicume0 - Outcome
- total lignanse0
- Value
- 1775–8965 µg g−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“twenty-eight sesame oil samples (1775–8965 µg g−1 total lignans”
Claim · model-written summary
Sesame oil samples contained 29.7–687.9 µg g−1 total tocopherols.
Not reportedTaxon
Verbatim
e0“29.7–687.9 µg g−1 total tocopherols”
Claim · model-written summary
Sesame oil samples contained 2640–9500 µg g−1 phytosterol.
Not reportedTaxon
Verbatim
e0“2640–9500 µg g−1 phytosterol”
Claim · model-written summary
Sesame oil samples contained 245–4030 µg g−1 squalene.
- Taxon
- SesameSesamum indicumt0
- Outcome
- squalenee0
- Value
- 245–4030 µg g−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“245–4030 µg g−1 squalene”
t0“Sesame Oil”