Paper · 2024 · Journal of Advances in Biology & Biotechnology

Indole Acetic Acid (IAA) Mediated Plant Growth Promotion Activity of Fluorescent Pseudomonads and Bacillus spp.

Kahkashan Arzoo; Rekha Balodi; Erayya; et al.

13
claims
32
verbatim quotes
1
citations
3
research areas
✓
Open access

Abstract

This investigation was made to study the involvement of IAA in plant growth promotion and vigor of wheat plants induced with plant growth promoting rhizobacteria. Nineteen isolates of fluorescent pseudomonads and 11 isolates of Bacillus spp. were subjected to germination percentage and seedling vigor index test. These parameters were determined according to recommended methods by ISTA (1985). The IAA production from selected bacterial isolates, which were showing the potential function of plant growth promotion were further subjected to quantitative analysis using Salkowski’s reagent (2% of 0.5 M FeCl3 in 34% HClO4) for Colorimetric detection. The vigor index and plant growth were significantly increased in Pfa-65 (43.36%) followed by B-18 (42.58%), Pfa-77 (40.83%), B-24 (40.68), Pfa-37(38.82%) and B-38 (38.52). The maximum concentration of IAA was detected in the isolate B-24(36.66 µg/ml) followed by Pfa-65(36.16 µg/ml), B-18(35.98 µg/ml) and Pfa-77(35.72 µg/ml). A positive correlation was observed between concentration of IAA produced and germination percentage with PGPR treatments (correlation coefficient was 0.94). The correlation coefficient was 0.96 between IAA produced and percent increase in vigor index with the treatments. Our data suggests that IAA, a growth promoting hormone, might be the key factor to promote the plant growth directed by the rhizobacteria

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Claims 13

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Pfa-65 increased wheat vigor index and growth by 43.36%.

Treatment
Pfa-65e0
Interactor
Pfa-65e0symbiont
Outcome
vigor index and plant growthe0
Direction
increasede0
Value
43.36%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The vigor index and plant growth were significantly increased in Pfa-65 (43.36%)”

✓ located verbatim in the source · abstract, characters 652–732 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

B-18 increased wheat vigor index and growth by 42.58%.

Treatment
B-18e0
Interactor
B-18e0symbiont
Outcome
vigor index and plant growthe0
Direction
increasede0
Value
42.58%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The vigor index and plant growth were significantly increased in Pfa-65 (43.36%) followed by B-18 (42.58%)”

✓ located verbatim in the source · abstract, characters 652–758 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Pfa-77 increased wheat vigor index and growth by 40.83%.

Treatment
Pfa-77e0
Interactor
Pfa-77e0symbiont
Outcome
vigor index and plant growthe0
Direction
increasede0
Value
40.83%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The vigor index and plant growth were significantly increased in Pfa-65 (43.36%) followed by B-18 (42.58%), Pfa-77 (40.83%)”

✓ located verbatim in the source · abstract, characters 652–775 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

B-24 increased wheat vigor index and growth by 40.68.

Treatment
B-24e0
Interactor
B-24e0symbiont
Outcome
vigor index and plant growthe1
Direction
increasede1
Value
40.68e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pfa-77 (40.83%), B-24 (40.68), Pfa-37(38.82%) and B-38 (38.52)”

✓ located verbatim in the source · abstract, characters 760–822 · show in text ↑
e1

“The vigor index and plant growth were significantly increased”

✓ located verbatim in the source · abstract, characters 652–713 · show in text ↑
e2

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Pfa-37 increased wheat vigor index and growth by 38.82%.

Treatment
Pfa-37e0
Interactor
Pfa-37e0symbiont
Outcome
vigor index and plant growthe1
Direction
increasede1
Value
38.82%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pfa-77 (40.83%), B-24 (40.68), Pfa-37(38.82%) and B-38 (38.52)”

✓ located verbatim in the source · abstract, characters 760–822 · show in text ↑
e1

“The vigor index and plant growth were significantly increased”

✓ located verbatim in the source · abstract, characters 652–713 · show in text ↑
e2

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

B-38 increased wheat vigor index and growth by 38.52.

Treatment
B-38e0
Interactor
B-38e0symbiont
Outcome
vigor index and plant growthe1
Direction
increasede1
Value
38.52e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pfa-77 (40.83%), B-24 (40.68), Pfa-37(38.82%) and B-38 (38.52)”

✓ located verbatim in the source · abstract, characters 760–822 · show in text ↑
e1

“The vigor index and plant growth were significantly increased”

✓ located verbatim in the source · abstract, characters 652–713 · show in text ↑
e2

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

B-24 produced the highest reported IAA concentration, 36.66 µg/ml.

Interactor
B-24e0symbiont
Outcome
concentration of IAAe0
Value
36.66 µg/mle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The maximum concentration of IAA was detected in the isolate B-24(36.66 µg/ml)”

✓ located verbatim in the source · abstract, characters 824–902 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Pfa-65 produced 36.16 µg/ml IAA.

Interactor
Pfa-65e0symbiont
Outcome
concentration of IAAe1
Value
36.16 µg/mle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“B-24(36.66 µg/ml) followed by Pfa-65(36.16 µg/ml)”

✓ located verbatim in the source · abstract, characters 885–934 · show in text ↑
e1

“The maximum concentration of IAA was detected in the isolate B-24(36.66 µg/ml)”

✓ located verbatim in the source · abstract, characters 824–902 · show in text ↑
e2

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

B-18 produced 35.98 µg/ml IAA.

Interactor
B-18e0symbiont
Outcome
concentration of IAAe1
Value
35.98 µg/mle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“B-18(35.98 µg/ml)”

✓ located verbatim in the source · abstract, characters 936–953 · show in text ↑
e1

“The maximum concentration of IAA was detected in the isolate B-24(36.66 µg/ml)”

✓ located verbatim in the source · abstract, characters 824–902 · show in text ↑
e2

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Pfa-77 produced 35.72 µg/ml IAA.

Interactor
Pfa-77e0symbiont
Outcome
concentration of IAAe1
Value
35.72 µg/mle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pfa-77(35.72 µg/ml)”

✓ located verbatim in the source · abstract, characters 958–977 · show in text ↑
e1

“The maximum concentration of IAA was detected in the isolate B-24(36.66 µg/ml)”

✓ located verbatim in the source · abstract, characters 824–902 · show in text ↑
e2

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

IAA production and wheat germination percentage were positively correlated, r=0.94.

Treatment
PGPR treatmentse0
Compound
IAAe0
Outcome
germination percentagee0
Direction
positive correlatione0
Value
0.94e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A positive correlation was observed between concentration of IAA produced and germination percentage with PGPR treatments (correlation coefficient was 0.94).”

✓ located verbatim in the source · abstract, characters 979–1136 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

IAA production and percent vigor-index increase were correlated, r=0.96.

Compound
IAAe0
Outcome
percent increase in vigor indexe0
Direction
increasee0
Value
0.96e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The correlation coefficient was 0.96 between IAA produced and percent increase in vigor index with the treatments.”

✓ located verbatim in the source · abstract, characters 1137–1251 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑
Mechanismhypothesisoriginal resultpositivemeasured

Claim · model-written summary

Authors suggest IAA may mediate growth promotion by rhizobacteria.

Interactor
rhizobacteriaHyphomicrobialese0symbiont
Compound
IAAe0
Outcome
plant growthe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“IAA, a growth promoting hormone, might be the key factor to promote the plant growth directed by the rhizobacteria”

✓ located verbatim in the source · abstract, characters 1275–1389 · show in text ↑
e1

“wheat plants”

✓ located verbatim in the source · abstract, characters 99–111 · show in text ↑