Abstract
The pathogenicity of grapevine geminivirus A (GGVA), a recently identified DNA virus, to grapevine plants remains largely unclear. Here, we report a new GGVA isolate (named GGVAQN) obtained from grapevine ‘Queen Nina’ plants with severe disease symptoms. The infectious clone of GGVAQN (pXT-GGVAQN) was constructed to investigate its pathogenicity. Nicotiana benthamiana plants inoculated with GGVAQN by agroinfiltration displayed upward leaf curling and chlorotic mottling symptoms. A simple, quick, and efficient method for delivering DNA clones of GGVAQN into grapevine plants was developed, by which Agrobacterium tumefaciens cells carrying pXT-GGVAQN were introduced into the roots of in vitro-grown ‘Red Globe’ grape plantlets with a syringe. By this method, all ‘Red Globe’ grape plants were systemically infected with GGVAQN, and the plants exhibited chlorotic mottling symptoms on their upper leaves and downward curling, interveinal yellowing, and leaf-margin necrosis symptoms on their lower leaves. Our results provide insights into the pathogenicity of GGVA and a simple and efficient inoculation method to deliver infectious viral clones to woody perennial plants.
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Claims 5
Claim · model-written summary
Nicotiana benthamiana plants inoculated with GGVAQN by agroinfiltration showed upward leaf curling and chlorotic mottling symptoms.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- inoculated with GGVAQN by agroinfiltratione0
- Interactor
- GGVAQNe0pathogen
- Outcome
- upward leaf curling and chlorotic mottling symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana plants inoculated with GGVAQN by agroinfiltration displayed upward leaf curling and chlorotic mottling symptoms”
Claim · model-written summary
A root syringe inoculation method systemically infected all Red Globe grape plants with GGVAQN.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Geminivirus”
e1“By this method, all ‘Red Globe’ grape plants were systemically infected with GGVAQN”
Claim · model-written summary
Grapevine plants infected with GGVAQN showed chlorotic mottling symptoms on upper leaves.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Geminivirus”
e2“the plants exhibited chlorotic mottling symptoms on their upper leaves and downward curling, interveinal yellowing, and leaf-margin necrosis symptoms on their lower leaves”
Claim · model-written summary
Grapevine plants infected with GGVAQN showed downward curling, interveinal yellowing and leaf-margin necrosis on lower leaves.
- Organ / stage
- lower leavese2
- Outcome
- downward curling, interveinal yellowing, and leaf-margin necrosis symptomse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Geminivirus”
e2“the plants exhibited chlorotic mottling symptoms on their upper leaves and downward curling, interveinal yellowing, and leaf-margin necrosis symptoms on their lower leaves”
Claim · model-written summary
A new grapevine geminivirus A isolate, GGVAQN, was identified from grapevine Queen Nina plants with severe disease symptoms.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Geminivirus”
e3“Here, we report a new GGVA isolate (named GGVAQN) obtained from grapevine ‘Queen Nina’ plants with severe disease symptoms”
The same questions in other papers
inoculation · disease · Nicotiana benthamianaPositive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- negative
- positive
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.