Abstract
Wheat powdery mildew is an important fungal disease that seriously jeopardizes wheat production, which poses a serious threat to food safety. SJ106 is a high-quality, disease-resistant spring wheat variety; this disease resistance is derived from Wheat-wheatgrass 33. In this study, the powdery mildew resistance genes in SJ106 were located at the end of chromosome 6DS, a new disease resistance locus tentatively named PmSJ106 locus. This interval was composed of a nucleotide-binding leucine-rich repeat (NLR) gene cluster containing 19 NLR genes. Five NLRs were tandem duplicated genes, and one of them (a coiled coil domain–nucleotide binding site–leucine-rich repeat (CC-NBS-LRR; CNL) type gene, TaRGA5-like) expressed 69–836-fold in SJ106 compared with the susceptible control. The genome DNA and cDNA sequences of TaRGA5-like were amplified from SJ106, which contain several nucleotide polymorphisms in LRR regions compared with susceptible individuals and Chinese Spring. Overexpression of TaRGA5-like significantly increased resistance to powdery mildew in susceptible receptor wheat Jinqiang5. However, Virus induced gene silence (VIGS) of TaRGA5-like resulted in only a small decrease of SJ106 in disease resistance, presumably compensated by other NLR duplicated genes. The results suggested that TaRGA5-like confers partial powdery mildew resistance in SJ106. As a member of the PmSJ106 locus, TaRGA5-like functioned together with other NLR duplicated genes to improve wheat resistance to powdery mildew. Wheat variety SJ106 would become a novel and potentially valuable germplasm for powdery mildew resistance.
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Claims 8
Claim · model-written summary
Powdery mildew resistance genes in SJ106 mapped to a new locus at the end of chromosome 6DS.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- SJ106e0
- Gene
- PmSJ106 locuse0
- Outcome
- powdery mildew resistance genese0
Not reportedSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the powdery mildew resistance genes in SJ106 were located at the end of chromosome 6DS, a new disease resistance locus tentatively named PmSJ106 locus”
t0“Wheat SJ106”
Claim · model-written summary
The mapped interval contains 19 NLR genes, including five tandem duplicates.
- Taxon
- WheatTriticum aestivumt0
- Gene
- NLR genese0
- Outcome
- (NLR) gene clustere0
- Value
- 19 NLR genese0
- Qualifier
- Five NLRs were tandem duplicated genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This interval was composed of a nucleotide-binding leucine-rich repeat (NLR) gene cluster containing 19 NLR genes. Five NLRs were tandem duplicated genes”
t0“Wheat SJ106”
Claim · model-written summary
TaRGA5-like expression in SJ106 was 69–836-fold that in the susceptible control.
- Taxon
- WheatTriticum aestivumt0
- Material
- SJ106e0
- Gene
- TaRGA5-likee0
- Outcome
- expressede0
- Direction
- expressed 69–836-folde0
- Value
- 69–836-folde0
- Comparator
- susceptible controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaRGA5-like) expressed 69–836-fold in SJ106 compared with the susceptible control”
t0“Wheat SJ106”
Claim · model-written summary
TaRGA5-like sequences in SJ106 have LRR-region polymorphisms relative to susceptible individuals and Chinese Spring.
- Taxon
- WheatTriticum aestivumt0
- Material
- SJ106e0
- Gene
- TaRGA5-likee0
- Outcome
- nucleotide polymorphisms in LRR regionse0
- Comparator
- susceptible individuals and Chinese Springe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genome DNA and cDNA sequences of TaRGA5-like were amplified from SJ106, which contain several nucleotide polymorphisms in LRR regions compared with susceptible individuals and Chinese Spring”
t0“Wheat SJ106”
Claim · model-written summary
Overexpressing TaRGA5-like significantly increased powdery mildew resistance in Jinqiang5.
- Taxon
- wheatTriticum aestivume0
- Material
- Jinqiang5e0
- Treatment
- Overexpression of TaRGA5-likee0
- Gene
- TaRGA5-likee0
- Outcome
- resistance to powdery mildewe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaRGA5-like significantly increased resistance to powdery mildew in susceptible receptor wheat Jinqiang5”
Claim · model-written summary
Silencing TaRGA5-like produced only a small decrease in SJ106 disease resistance.
- Taxon
- WheatTriticum aestivumt0
- Material
- SJ106e0
- Treatment
- Virus induced gene silence (VIGS) of TaRGA5-likee0
- Gene
- TaRGA5-likee0
- Outcome
- disease resistancee0
- Direction
- decreasee0
- Qualifier
- only a small decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus induced gene silence (VIGS) of TaRGA5-like resulted in only a small decrease of SJ106 in disease resistance”
t0“Wheat SJ106”
Claim · model-written summary
The authors infer that TaRGA5-like acts with other duplicated NLR genes to improve resistance to powdery mildew.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaRGA5-likee0
- Outcome
- resistance to powdery mildewe0
- Qualifier
- together with other NLR duplicated genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaRGA5-like functioned together with other NLR duplicated genes to improve wheat resistance to powdery mildew”
Claim · model-written summary
The authors identify SJ106 as potentially valuable germplasm for powdery mildew resistance.
- Taxon
- WheatTriticum aestivume0
- Material
- SJ106e0
- Outcome
- valuable germplasm for powdery mildew resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat variety SJ106 would become a novel and potentially valuable germplasm for powdery mildew resistance”
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loss of function · disease · Triticum aestivum (wheat)Positive and negative results
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