Abstract
Tree roots are associated with a high diversity of microorganisms that play key roles in soil fertility and plant nutrition and health. Here we examine the composition and diversity of bacterial community in ectomycorrhizal root (ECR) and their ectomycorrhizosphere (EMR) of the Neotropical tree Pinus montezumae inoculated with Laccaria laccata and Hebeloma mesophaeum, applied alone or co-inoculated with Azospirillum brasilense. For this, we used a 16S rRNA gene metabarcoding-sequencing approach in samples of ECR and EMR that were collected nine-months after inoculation in greenhouse. A total of 943 Operational Taxonomic Units (OTU) were differentiated and grouped in 32 bacterial phyla. The most abundant phyla were Proteobacteria (67%) and Actinobacteria (20.7%). Bacterial communities were structured in ECR and EMR (Anosim, R=0.77, P≤0.005), and were more diverse in EMR than ECR (Shannon-Weaver of 4.2 and 3.6, and Simpson inverse of 33.7 and 16.7, respectively). The abundance of Pseudomonas, Actinoplanes and Salinibacterium were promoted with L. laccata, while with H. mesophaeum an OTU from Burkholderiaceae was prominent. Our results showed that the ectomycorrhizal fungi strongly shaped the composition of the bacterial communities associated with roots of P. montezumae.
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Claims 8
Claim · model-written summary
The samples contained 943 bacterial OTUs in 32 phyla.
- Taxon
- Pinus montezumae
Pinus montezumaet0 - Outcome
- Operational Taxonomic Units (OTU)e0
- Value
- 943 Operational Taxonomic Units (OTU)e0
- Qualifier
- 32 bacterial phylae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 943 Operational Taxonomic Units (OTU) were differentiated and grouped in 32 bacterial phyla.”
t0“Pinus montezumae”
Claim · model-written summary
Proteobacteria accounted for 67% of the bacterial community.
- Taxon
- Pinus montezumaePinus montezumaet0
- Outcome
- Proteobacteriae0
- Value
- 67%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most abundant phyla were Proteobacteria (67%) and Actinobacteria (20.7%).”
t0“Pinus montezumae”
Claim · model-written summary
Actinobacteria accounted for 20.7% of the bacterial community.
- Taxon
- Pinus montezumaePinus montezumaet0
- Outcome
- Actinobacteriae0
- Value
- 20.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most abundant phyla were Proteobacteria (67%) and Actinobacteria (20.7%).”
t0“Pinus montezumae”
Claim · model-written summary
Bacterial community structure differed between ectomycorrhizal roots and their ectomycorrhizosphere.
- Taxon
- Pinus montezumaePinus montezumaet0
- Organ / stage
- ECR and EMRe0
- Outcome
- Bacterial communitiese0
- Value
- R=0.77e0
- Qualifier
- P≤0.005e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacterial communities were structured in ECR and EMR (Anosim, R=0.77, P≤0.005)”
t0“Pinus montezumae”
Claim · model-written summary
Bacterial communities were more diverse in the ectomycorrhizosphere than in ectomycorrhizal roots.
- Taxon
- Pinus montezumaePinus montezumaet0
- Organ / stage
- EMRe0
- Outcome
- diversee0
- Direction
- more diversee0
- Value
- 4.2 and 3.6e0
- Qualifier
- 33.7 and 16.7e0
- Comparator
- ECRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were more diverse in EMR than ECR (Shannon-Weaver of 4.2 and 3.6, and Simpson inverse of 33.7 and 16.7, respectively).”
t0“Pinus montezumae”
Claim · model-written summary
L. laccata promoted Pseudomonas, Actinoplanes, and Salinibacterium abundance.
- Taxon
- Pinus montezumaePinus montezumaet0
- Treatment
- L. laccatae0
- Interactor
- L. laccata
Laccaria laccatae0symbionte1 - Outcome
- abundance of Pseudomonas, Actinoplanes and Salinibacteriume0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abundance of Pseudomonas, Actinoplanes and Salinibacterium were promoted with L. laccata”
e1“Pinus montezumae inoculated with Laccaria laccata and Hebeloma mesophaeum, applied alone or co-inoculated with Azospirillum brasilense.”
t0“Pinus montezumae”
Claim · model-written summary
An OTU from Burkholderiaceae was prominent with H. mesophaeum.
- Taxon
- Pinus montezumaePinus montezumaet0
- Interactor
- H. mesophaeum
Hebeloma mesophaeume0symbionte1 - Outcome
- an OTU from Burkholderiaceae was prominente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with H. mesophaeum an OTU from Burkholderiaceae was prominent.”
e1“Pinus montezumae inoculated with Laccaria laccata and Hebeloma mesophaeum, applied alone or co-inoculated with Azospirillum brasilense.”
t0“Pinus montezumae”
Claim · model-written summary
The authors conclude that ectomycorrhizal fungi strongly shaped bacterial community composition at P. montezumae roots.
- Taxon
- P. montezumaePinus montezumaee0
- Interactor
- ectomycorrhizal fungie0symbiont
- Outcome
- composition of the bacterial communities associated with rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ectomycorrhizal fungi strongly shaped the composition of the bacterial communities associated with roots of P. montezumae.”