Abstract
ARs plays a crucial role in plant morphogenesis and development. The limited and inefficient rooting of scions poses a significant challenge to the efficiency and quality of clonal propagation of forest trees in silvicultural practices. Building on previous research conducted by our team, we found that applying IBA at a concentration of 1000 mg/L significantly enhanced mulberry rooting. This study aims to uncover the molecular mechanisms underlying this effect by analyzing RNA sequencing data from mulberry phloem before and after treatment with IBA over time intervals of 10, 20, 30, and 40 days. We identified 5226 DEGs, which were then classified into GO terms and KEGG pathways, showing significant enrichment in hormone signaling processes. Using WGCNA, we identified eight co-expression modules, two of which were significantly correlated with the IBA treatment. Additionally, 18 transcription factors that potentially facilitate ARs formation in mulberry were identified, and an exploratory analysis on the cis-regulatory elements associated with these transcription factors was conducted. The findings of this study provide a comprehensive understanding of the mechanisms of ARs in mulberry and offer theoretical support for the discovery and utilization of exceptional genetic resources within the species.
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Claims 5
Claim · model-written summary
IBA at 1000 mg/L significantly enhanced mulberry rooting.
Not reportedComparator
Verbatim
e0“applying IBA at a concentration of 1000 mg/L significantly enhanced mulberry rooting.”
Claim · model-written summary
The mulberry RNA-seq analysis identified 5226 differentially expressed genes.
Not reportedTaxon
Verbatim
e0“We identified 5226 DEGs, which were then classified into GO terms and KEGG pathways, showing significant enrichment in hormone signaling processes.”
Claim · model-written summary
The identified mulberry genes showed significant enrichment in hormone signaling processes.
- Outcome
- enrichment in hormone signaling processese0
Not reportedTaxon
Verbatim
e0“showing significant enrichment in hormone signaling processes.”
Claim · model-written summary
Two of eight mulberry co-expression modules correlated significantly with IBA treatment.
- Treatment
- IBA treatmente0
- Outcome
- co-expression modulese0
- Direction
- correlatede0
- Value
- twoe0
- Qualifier
- eighte0
Not reportedTaxon
Verbatim
e0“Using WGCNA, we identified eight co-expression modules, two of which were significantly correlated with the IBA treatment.”
Claim · model-written summary
The analysis identified 18 transcription factors potentially facilitating adventitious root formation.
Verbatim
e0“18 transcription factors that potentially facilitate ARs formation in mulberry were identified”
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