Paper · 2024 · Biotechnology Journal International

Optimization of Plant Growth Regulators in Indirect Plant Regeneration in Rice Variety Nerica 3 Using Mature Seeds as Explant

Danielle Christelle Tinak Ekom; Haïcha Diko Abba; Jean Cyrille Mouen Piau; et al.

3
claims
8
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

The study is the first attempt to highlight about callus induction and plantlet regeneration in rice variety ‘Nerica 3’ using mature seed as explant and using five regeneration media with plant growth regulators (BA, IAA, and 2,4-D) at different concentrations in other to establish the efficient indirect plant regeneration protocol. Among the five regeneration media tested, R3 medium (2 mg/L BA), showed greater percentage of plantlet regeneration (45.31%) followed by R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%), whereas, R4 medium showed the lowest (2 mg/L 2,4-D; 0%). These results will be used for genetic improvement programs of the variety ‘Nerica 3’.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

R3 medium with 2 mg/L BA gave the greatest plantlet regeneration, 45.31%.

Taxon
rice variety Nerica 3Oryza sativat0
Material
Nerica 3t0
Treatment
R3 medium (2 mg/L BA)e0
Outcome
percentage of plantlet regeneratione0
Direction
greatere0
Value
45.31%e0
Comparator
R2 mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“R3 medium (2 mg/L BA), showed greater percentage of plantlet regeneration (45.31%) followed by R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%)”

✓ located verbatim in the source · abstract, characters 377–518 · show in text ↑
t0

“rice variety Nerica 3”

✓ located after whitespace/quote normalisation · title, characters 74–95 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

R2 medium with BA and IAA produced 29.68% plantlet regeneration.

Treatment
R2 medium (0.5 mg/L BA + 0,1 mg/L IAAe0
Outcome
plantlet regenerationt1
Value
29.68%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%)”

✓ located verbatim in the source · abstract, characters 472–518 · show in text ↑
t0

“rice variety Nerica 3”

✓ located after whitespace/quote normalisation · title, characters 74–95 · show in text ↑
t1

“percentage of plantlet regeneration”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

R4 medium with 2 mg/L 2,4-D had the lowest plantlet regeneration, 0%.

Treatment
2 mg/L 2,4-De0
Outcome
plantlet regenerationt1
Direction
loweste0
Value
0%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“whereas, R4 medium showed the lowest (2 mg/L 2,4-D; 0%)”

✓ located verbatim in the source · abstract, characters 520–575 · show in text ↑
t0

“rice variety Nerica 3”

✓ located after whitespace/quote normalisation · title, characters 74–95 · show in text ↑
t1

“percentage of plantlet regeneration”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑

N-benzyladenine · growth · Oryza sativa (rice)Results differ

72 papers: 68 positive, 4 negative or no effect

67 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

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2,4-D · growth · Oryza sativa (rice)Positive and negative results

107 papers: 102 positive, 5 negative or no effect

4 papers in the same direction as this paper · 102 in the opposite direction

  1. positive
  2. positive
  3. positive

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in vitro · growth · Oryza sativa (rice)Positive and negative results

165 papers: 140 positive, 25 negative or no effect

139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

161 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.