Abstract
Xanthomonas citri subsp. citri ( Xcc ), the causal agent of citrus canker, elicits canker symptoms in citrus plants because of the transcriptional activator-like (TAL) effector PthA4, which activates the expression of the citrus susceptibility gene CsLOB1 . This study reports the regulation of the putative carbohydrate-binding protein gene Cs9g12620 by PthA4-mediated induction of CsLOB1 during Xcc infection. We found that the transcription of Cs9g12620 was induced by infection with Xcc in a PthA4-dependent manner. Even though it specifically bound to a putative TAL effector-binding element in the Cs9g12620 promoter, PthA4 exerted a suppressive effect on the promoter activity. In contrast, CsLOB1 bound to the Cs9g12620 promoter to activate its expression. The silencing of CsLOB1 significantly reduced the level of expression of Cs9g12620 , which demonstrated that Cs9g12620 was directly regulated by CsLOB1. Intriguingly, PhtA4 interacted with CsLOB1 and exerted feedback control that suppressed the induction of expression of Cs9g12620 by CsLOB1. Transient overexpression and gene silencing revealed that Cs9g12620 was required for the optimal development of canker symptoms. These results support the hypothesis that the expression of Cs9g12620 is dynamically directed by PthA4 for canker formation through the PthA4-mediated induction of CsLOB1.
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Claims 7
Claim · model-written summary
Xcc infection induced Cs9g12620 transcription in a PthA4-dependent manner.
- Interactor
- Xcce0pathogen
- Gene
- Cs9g12620e0
- Outcome
- transcription of Cs9g12620e0
- Direction
- inducede0
- Qualifier
- in a PthA4-dependent mannere0
Not reportedTaxon
Verbatim
e0“transcription of Cs9g12620 was induced by infection with Xcc in a PthA4-dependent manner”
e1“citrus plants”
Claim · model-written summary
PthA4 bound a putative effector binding element in the Cs9g12620 promoter.
Not reportedTaxon
Verbatim
e0“it specifically bound to a putative TAL effector-binding element in the Cs9g12620 promoter”
e1“citrus plants”
Claim · model-written summary
PthA4 suppressed Cs9g12620 promoter activity.
Not reportedTaxonGeneComparator
Verbatim
e0“PthA4 exerted a suppressive effect on the promoter activity”
e1“citrus plants”
Claim · model-written summary
CsLOB1 bound the Cs9g12620 promoter and activated expression.
Not reportedTaxon
Verbatim
e0“CsLOB1 bound to the Cs9g12620 promoter to activate its expression”
e1“citrus plants”
Claim · model-written summary
Silencing CsLOB1 significantly reduced Cs9g12620 expression.
- Treatment
- silencing of CsLOB1e0
- Gene
- Cs9g12620e0
- Outcome
- level of expression of Cs9g12620e0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“The silencing of CsLOB1 significantly reduced the level of expression of Cs9g12620”
e1“citrus plants”
Claim · model-written summary
PhtA4 interacted with CsLOB1 and suppressed CsLOB1 induction of Cs9g12620 expression.
- Gene
- CsLOB1e0
- Outcome
- induction of expression of Cs9g12620e0
- Direction
- suppressede0
- Qualifier
- PhtA4 interacted with CsLOB1e0
Not reportedTaxon
Verbatim
e0“PhtA4 interacted with CsLOB1 and exerted feedback control that suppressed the induction of expression of Cs9g12620 by CsLOB1”
e1“citrus plants”
Claim · model-written summary
Cs9g12620 was required for optimal canker symptom development.
Not reportedTaxon
Verbatim
e0“Cs9g12620 was required for the optimal development of canker symptoms”
e1“citrus plants”