Abstract
Superoxide dismutase (SOD) is widely present in plants and plays a crucial role in defending against oxidative stress and preventing tissue damage. This study discovered that the PagSOD2a gene in 84K poplar ( Populus alba × P. glandulosa ) exhibits a distinct capacity to be induced in response to salt stress. To delve into the pivotal role of PagSOD2a in conferring salt tolerance, the entire PagSOD2a fragment was successfully cloned from 84K poplar and the potential function of PagSOD2a was explored using bioinformatics and subcellular localization. PagSOD2a was found to encode a CuZn-SOD protein localized in chloroplasts. Furthermore, six CuZn-SOD family members were identified in poplar, with closely related members displaying similar gene structures, indicating evolutionary conservation. Morphological and physiological indexes of transgenic 84K poplar overexpressing PagSOD2a (OE) were compared with non-transgenic wild-type (WT) plants under salt stress. The OE lines (OE1 and OE3) showed improved growth performance, characterized by increased plant height and fresh weight, along with reduced malondialdehyde (MDA) content and electrolyte leakage rate under salt stress. Meanwhile, overexpression of PagSOD2a significantly augmented CuZn-SOD and total SOD enzyme activities, leading to a reduction in superoxide anion accumulation and an enhancement of salt tolerance. Additionally, co-expression and multilayered hierarchical gene regulatory network (ML-hGRN) mediated by PagSOD2a constructed using transcriptome data revealed that PagSOD2a gene may be directly regulated by SPL13 , NGA1b and FRS5 , as well as indirectly regulated by MYB102 and WRKY6 , in response to salt stress. These findings provide a theoretical and material foundation for further elucidating the function of PagSOD2a under salt stress and for developing salt-tolerant poplar varieties.
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Claims 11
Claim · model-written summary
PagSOD2a is induced by salt stress in 84K poplar.
Verbatim
e0“the PagSOD2a gene in 84K poplar ( Populus alba × P. glandulosa ) exhibits a distinct capacity to be induced in response to salt stress.”
Claim · model-written summary
PagSOD2a encodes a chloroplast-localized CuZn-SOD protein.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PagSOD2a was found to encode a CuZn-SOD protein localized in chloroplasts.”
t0“poplar”
Claim · model-written summary
Six CuZn-SOD family members were identified in poplar.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six CuZn-SOD family members were identified in poplar”
Claim · model-written summary
Closely related CuZn-SOD members had similar gene structures.
- Outcome
- similar gene structurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“closely related members displaying similar gene structures”
t0“poplar”
Claim · model-written summary
PagSOD2a overexpression increased plant height and fresh weight under salt stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OE lines (OE1 and OE3) showed improved growth performance, characterized by increased plant height and fresh weight, along with reduced malondialdehyde (MDA) content and electrolyte leakage rate under salt stress.”
t0“poplar”
Claim · model-written summary
PagSOD2a overexpression reduced malondialdehyde content and electrolyte leakage under salt stress.
- Material
- OE1 and OE3e0
- Treatment
- OEe0
- Outcome
- malondialdehyde (MDA) content and electrolyte leakage ratee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OE lines (OE1 and OE3) showed improved growth performance, characterized by increased plant height and fresh weight, along with reduced malondialdehyde (MDA) content and electrolyte leakage rate under salt stress.”
t0“poplar”
Claim · model-written summary
PagSOD2a overexpression significantly increased CuZn-SOD and total SOD activities.
- Treatment
- overexpression of PagSOD2ae0
- Gene
- PagSOD2ae0
- Outcome
- CuZn-SOD and total SOD enzyme activitiese0
- Direction
- augmentede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PagSOD2a significantly augmented CuZn-SOD and total SOD enzyme activities”
t0“poplar”
Claim · model-written summary
PagSOD2a overexpression reduced superoxide anion accumulation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PagSOD2a significantly augmented CuZn-SOD and total SOD enzyme activities, leading to a reduction in superoxide anion accumulation and an enhancement of salt tolerance.”
t0“poplar”
Claim · model-written summary
PagSOD2a overexpression enhanced salt tolerance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PagSOD2a significantly augmented CuZn-SOD and total SOD enzyme activities, leading to a reduction in superoxide anion accumulation and an enhancement of salt tolerance.”
t0“poplar”
Claim · model-written summary
Network analysis suggested direct regulation of PagSOD2a by SPL13, NGA1b and FRS5.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PagSOD2a gene may be directly regulated by SPL13 , NGA1b and FRS5”
t0“poplar”
Claim · model-written summary
Network analysis suggested indirect regulation of PagSOD2a by MYB102 and WRKY6.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as well as indirectly regulated by MYB102 and WRKY6 , in response to salt stress.”
t0“PagSOD2a improves poplar salt tolerance”