Abstract
Light is crucial for flower bud development in plants, serving as both signal and energy source. However, the mechanisms by which daylength and light intensity regulate flowering in modern roses remain unclear. In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds. RNA sequencing identified the Trehalose-6-phosphate phosphatase F (RhTPPF) gene as a key responder to insufficient light, modulating Tre6P metabolism. Overexpression of RhTPPF in rose calli enhanced sugar accumulation and suppressed the synthesis of RhCO/FT. In tobacco, overexpression of RhTPPF delayed the transition from vegetative growth to flowering, while silencing RhTPPF in roses accelerated flowering. Silencing RhTPPF in roses elevated trehalose-6-phosphate (Tre6P) levels and decreased trehalose. Transcriptome data showed that the expression level of RhTPPF was highly correlated with the circadian rhythm gene LATE ELONGATED HYPOCOTYL (RhLHY). Yeast one-hybrid assays, dual luciferase assays and EMSA revealed that RhLHY directly binds to the RhTPPF promoters. Overexpression of RhLHY suppressed flowering, while silencing RhLHY promoted flowering. Furthermore, altering the expression of RhLHY influenced Tre6P synthesis and the expression of sucrose-related transport genes. These findings suggest a RhLHY-RhTPPF-Tre6P regulatory module that maintains sugar balance and inhibits flower formation under reduced light conditions by modulating sugar distribution.
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Claims 16
Claim · model-written summary
Insufficient light delays flowering in Rosa hybrida Carola.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare1 - Treatment
- insufficient lighte1
- Outcome
- floweringe1
- Direction
- delayse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
Claim · model-written summary
Insufficient light reduces the sugar content in terminal buds of Rosa hybrida Carola.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Organ / stage
- terminal budse1
- Treatment
- insufficient lighte1
- Outcome
- sugar content in terminal budse1
- Direction
- reducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
Claim · model-written summary
RhTPPF was identified as a key responder to insufficient light.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Treatment
- insufficient lighte2
- Gene
- RhTPPFe2
- Outcome
- key respondere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e2“RNA sequencing identified the Trehalose-6-phosphate phosphatase F (RhTPPF) gene as a key responder to insufficient light, modulating Tre6P metabolism”
Claim · model-written summary
Overexpression of RhTPPF in rose calli enhanced sugar accumulation.
- Taxon
- rosee3
- Treatment
- Overexpression of RhTPPFe3
- Gene
- RhTPPFe3
- Outcome
- sugar accumulatione3
- Direction
- enhancede3
Not reportedComparator
Verbatim
e3“Overexpression of RhTPPF in rose calli enhanced sugar accumulation and suppressed the synthesis of RhCO/FT”
Claim · model-written summary
Overexpression of RhTPPF in rose calli suppressed the synthesis of RhCO/FT.
Not reportedComparator
Verbatim
e3“Overexpression of RhTPPF in rose calli enhanced sugar accumulation and suppressed the synthesis of RhCO/FT”
Claim · model-written summary
Silencing RhTPPF in roses accelerated flowering.
Not reportedComparator
Verbatim
e4“In tobacco, overexpression of RhTPPF delayed the transition from vegetative growth to flowering, while silencing RhTPPF in roses accelerated flowering”
Claim · model-written summary
Overexpression of RhTPPF in tobacco delayed the transition from vegetative growth to flowering.
- Taxon
- tobacco
Nicotiana tabacume4 - Treatment
- overexpression of RhTPPFe4
- Outcome
- transition from vegetative growth to floweringe4
- Direction
- delayede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In tobacco, overexpression of RhTPPF delayed the transition from vegetative growth to flowering, while silencing RhTPPF in roses accelerated flowering”
Claim · model-written summary
Silencing RhTPPF in roses elevated trehalose-6-phosphate (Tre6P) levels.
- Taxon
- rosese5
- Treatment
- Silencing RhTPPFe5
- Outcome
- trehalose-6-phosphate (Tre6P) levelse5
- Direction
- elevatede5
Not reportedComparator
Verbatim
e5“Silencing RhTPPF in roses elevated trehalose-6-phosphate (Tre6P) levels and decreased trehalose”
Claim · model-written summary
Silencing RhTPPF in roses decreased trehalose.
Not reportedComparator
Verbatim
e5“Silencing RhTPPF in roses elevated trehalose-6-phosphate (Tre6P) levels and decreased trehalose”
Claim · model-written summary
The expression level of RhTPPF was highly correlated with that of the circadian gene RhLHY in Rosa hybrida.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Gene
- RhLHYe6
- Outcome
- expression level of RhTPPFe6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e6“Transcriptome data showed that the expression level of RhTPPF was highly correlated with the circadian rhythm gene LATE ELONGATED HYPOCOTYL (RhLHY)”
Claim · model-written summary
RhLHY directly binds to the RhTPPF promoters.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Gene
- RhLHYe7
- Outcome
- binds to the RhTPPF promoterse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e7“Yeast one-hybrid assays, dual luciferase assays and EMSA revealed that RhLHY directly binds to the RhTPPF promoters”
Claim · model-written summary
Overexpression of RhLHY suppressed flowering.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Treatment
- Overexpression of RhLHYe6
- Outcome
- floweringe6
- Direction
- suppressede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e6“Overexpression of RhLHY suppressed flowering, while silencing RhLHY promoted flowering”
Claim · model-written summary
Silencing RhLHY promoted flowering.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Treatment
- silencing RhLHYe6
- Outcome
- floweringe6
- Direction
- promotede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e6“Overexpression of RhLHY suppressed flowering, while silencing RhLHY promoted flowering”
Claim · model-written summary
Altering the expression of RhLHY influenced Tre6P synthesis.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Treatment
- altering the expression of RhLHYe7
- Outcome
- Tre6P synthesise7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e7“Furthermore, altering the expression of RhLHY influenced Tre6P synthesis and the expression of sucrose-related transport genes”
Claim · model-written summary
Altering the expression of RhLHY influenced the expression of sucrose-related transport genes.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Treatment
- altering the expression of RhLHYe7
- Outcome
- expression of sucrose-related transport genese7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e7“Furthermore, altering the expression of RhLHY influenced Tre6P synthesis and the expression of sucrose-related transport genes”
Claim · model-written summary
A RhLHY-RhTPPF-Tre6P regulatory module inhibits flower formation under reduced light conditions by modulating sugar distribution.
- Taxon
- Rosa hybridaRosa hybrid cultivare1
- Gene
- RhLHY-RhTPPFe0
- Outcome
- flower formatione0
- Qualifier
- under reduced light conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In Rosa hybrida 'Carola', insufficient light delays flowering and reduces the sugar content in terminal buds”
e0“These findings suggest a RhLHY-RhTPPF-Tre6P regulatory module that maintains sugar balance and inhibits flower formation under reduced light conditions by modulating sugar distribution”
The same questions in other papers
loss of function · phenology · RosaPositive and negative results
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- negative
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light · phenology · Rosa hybrid cultivarPositive and negative results
6 in the opposite direction
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3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.