Abstract
• Alkaline stress significantly increased methyl jasmonate (MeJA) in rice seedlings. • Jasmonate-responsive genes were consistently activated by alkaline stress. • MeJA enhanced rice tolerance to alkaline stress through the auxin pathway. Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na 2 CO 3 ), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes. Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress. This treatment also upregulated genes associated with cell death suppression ( OsBI1 ) and stress tolerance ( OsJRL, OsNAC ). Notably, MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis ( OASA1, OASA2 ) and auxin signaling ( Aux/IAA, ARFs ). Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA. These results highlight the crucial role of MeJA-induced activation of auxin pathway in enhancing rice tolerance to alkaline stress, and provide valuable insights for developing strategies to improve crop resilience in alkaline soils.
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Claims 9
Claim · model-written summary
Alkaline stress elevated endogenous MeJA in rice seedlings.
- Taxon
- rice
Oryza sativae0 - Treatment
- alkaline stress (15 mM Na 2 CO 3 )e0
- Outcome
- levels of endogenous MeJAe0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under alkaline stress (15 mM Na 2 CO 3 ), rice seedlings exhibited elevated levels of endogenous MeJA”
Claim · model-written summary
Alkaline stress upregulated JA-responsive genes in rice seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- alkaline stress (15 mM Na 2 CO 3 )e0
- Outcome
- JA-responsive genese0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under alkaline stress (15 mM Na 2 CO 3 ), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes”
Claim · model-written summary
MeJA pretreatment improved rice seedling survival and growth under alkaline stress.
- Taxon
- riceOryza sativat0
- Treatment
- Pre-treatment with MeJA (50 µM)e0
- Outcome
- seedling survival, growthe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress”
t0“rice”
Claim · model-written summary
MeJA pretreatment mitigated rice root damage under alkaline stress.
- Taxon
- riceOryza sativat0
- Treatment
- Pre-treatment with MeJA (50 µM)e0
- Outcome
- root damagee0
- Direction
- mitigatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress”
t0“rice”
Claim · model-written summary
MeJA pretreatment upregulated OsBI1, OsJRL and OsNAC.
- Taxon
- riceOryza sativat0
- Treatment
- This treatmente0
- Outcome
- genes associated with cell death suppression ( OsBI1 ) and stress tolerance ( OsJRL, OsNAC )e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This treatment also upregulated genes associated with cell death suppression ( OsBI1 ) and stress tolerance ( OsJRL, OsNAC )”
t0“rice”
Claim · model-written summary
MeJA pretreatment increased root IAA levels.
- Taxon
- riceOryza sativat0
- Treatment
- MeJA pre-treatmente0
- Outcome
- auxin (indole-3-acetic acid, IAA) levels in rootse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots”
t0“rice”
Claim · model-written summary
MeJA pretreatment upregulated genes for IAA synthesis and auxin signaling.
- Taxon
- riceOryza sativat0
- Treatment
- MeJA pre-treatmente0
- Outcome
- genes involved in IAA synthesis ( OASA1, OASA2 ) and auxin signaling ( Aux/IAA, ARFs )e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis ( OASA1, OASA2 ) and auxin signaling ( Aux/IAA, ARFs )”
t0“rice”
Claim · model-written summary
Blocking auxin transport intensified root damage under alkaline stress.
- Taxon
- riceOryza sativat0
- Treatment
- Blocking auxin transport with N-1-naphthylphthalamic acide0
- Outcome
- root damagee0
- Direction
- intensifiede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress”
t0“rice”
Claim · model-written summary
Blocking auxin transport diminished the protective effect of MeJA.
- Taxon
- riceOryza sativat0
- Treatment
- Blocking auxin transport with N-1-naphthylphthalamic acide0
- Outcome
- protective effect of MeJAe0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA”
t0“rice”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.