Paper · 2024 · Acta Agrobotanica

Enrichment of broccoli seedlings with iron and its impact on germination, antioxidant activity, and the level of phenolic compounds

Adrianna Tomasik; Anna Kubacka; Şuheda Uğur; et al.

6
claims
12
verbatim quotes
0
citations
1
research areas
✓
Open access · cc-by

Abstract

Biofortification of edible seedlings (sprouts) is a promising strategy to combat dietary nutrient deficiency. In the present study, we made an attempt to enrich broccoli seedlings through imbibition with Fe at two concentrations, 100 mg Fe · dm–3 and 500 mg Fe · dm–3, of four chemical forms: FeCl2, FeCl3, FeSO4, or Fe2(SO4)3. The imbibition in all of the solutions resulted in a significant increase in Fe content in the seedlings, wherein it was the highest by the imbibition in sulphates applied at the highest concentration (500 mg·dm-3). In addition, the imbibition in sulphates in general did not affect the germination rate, whereas the imbibition in chlorites hampered the germination process. The application of Fe at a concentration of 500 mg Fe · dm–3 in the form of FeSO4 enhanced antioxidant activity, while the application of Fe in the form of FeCl2 and FeCl3 stimulated the accumulation of anthocyanins. It can be concluded that seed imbibition in Fe in the form of FeSO4 is an easy and cheap method for biofortification of broccoli seedlings, leading to a nearly 10-fold increase in Fe content and significant enhancement of antioxidant activity, without affecting the germination rate.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Fe imbibition significantly increases Fe content in broccoli seedlings, most with sulphates at 500 mg per dm3.

Taxon
broccoli seedlingsBrassica oleracea var. italicae0
Organ / stage
seedlingse1
Treatment
imbibition with Fee0
Outcome
Fe contente1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“we made an attempt to enrich broccoli seedlings through imbibition with Fe”

✓ located verbatim in the source · abstract, characters 132–206 · show in text ↑
e1

“The imbibition in all of the solutions resulted in a significant increase in Fe content in the seedlings”

✓ located verbatim in the source · abstract, characters 328–432 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Imbibition in sulphates in general does not affect the germination rate of broccoli seedlings.

Taxon
broccoli seedlingsBrassica oleracea var. italicae0
Treatment
imbibition in sulphatese1
Outcome
germination ratee1
Direction
did not affecte1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“we made an attempt to enrich broccoli seedlings through imbibition with Fe”

✓ located verbatim in the source · abstract, characters 132–206 · show in text ↑
e1

“the imbibition in sulphates in general did not affect the germination rate”

✓ located verbatim in the source · abstract, characters 557–631 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Imbibition in chlorites hampers the germination process of broccoli seedlings.

Taxon
broccoli seedlingsBrassica oleracea var. italicae0
Treatment
imbibition in chloritese1
Outcome
germination processe1
Direction
hamperede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“we made an attempt to enrich broccoli seedlings through imbibition with Fe”

✓ located verbatim in the source · abstract, characters 132–206 · show in text ↑
e1

“the imbibition in chlorites hampered the germination process”

✓ located verbatim in the source · abstract, characters 641–701 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fe at 500 mg per dm3 applied as FeSO4 enhances antioxidant activity in broccoli seedlings.

Taxon
broccoli seedlingsBrassica oleracea var. italicae0
Treatment
FeSO4e1
Outcome
antioxidant activitye1
Direction
enhancede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“we made an attempt to enrich broccoli seedlings through imbibition with Fe”

✓ located verbatim in the source · abstract, characters 132–206 · show in text ↑
e1

“in the form of FeSO4 enhanced antioxidant activity”

✓ located verbatim in the source · abstract, characters 764–814 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fe applied as FeCl2 and FeCl3 stimulates the accumulation of anthocyanins in broccoli seedlings.

Taxon
broccoli seedlingsBrassica oleracea var. italicae0
Treatment
FeCl2 and FeCl3e1
Outcome
accumulation of anthocyaninse1
Direction
stimulatede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“we made an attempt to enrich broccoli seedlings through imbibition with Fe”

✓ located verbatim in the source · abstract, characters 132–206 · show in text ↑
e1

“while the application of Fe in the form of FeCl2 and FeCl3 stimulated the accumulation of anthocyanins”

✓ located verbatim in the source · abstract, characters 816–918 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed imbibition in Fe as FeSO4 raises Fe content of broccoli seedlings nearly 10-fold.

Taxon
broccoli seedlingsBrassica oleracea var. italicae1
Treatment
seed imbibition in Fe in the form of FeSO4e1
Outcome
Fe contente0
Direction
increasee0
Value
nearly 10-folde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“leading to a nearly 10-fold increase in Fe content”

✓ located verbatim in the source · abstract, characters 1060–1110 · show in text ↑
e1

“seed imbibition in Fe in the form of FeSO4 is an easy and cheap method for biofortification of broccoli seedlings”

✓ located verbatim in the source · abstract, characters 945–1058 · show in text ↑

iron atom · nutrient: content · Brassica oleracea (wild cabbage)Results differ

7 papers: 6 positive, 1 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

iron(2+) sulfate (anhydrous) · quality: phytochemicals · Brassica oleracea (wild cabbage)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.