Paper · 2024 · Frontiers in Microbiology

Study on effect of plant growth promoting rhizobacteria on sorghum (Sorghum bicolor L.) under gnotobiotic conditions

M. Chiranjeevi; Geeta D. Goudar; Krishnaraj PU; et al.

10
claims
20
verbatim quotes
4
citations
3
research areas
✓
Open access · cc-by

Abstract

The rhizosphere is enriched with diverse microflora, allowing for delving prospective microorganisms to enhance crop growth and yield for varied soil conditions. Demand for millet growth-promoting microorganisms is a contemporary need for dryland agriculture. Therefore, a detailed survey was conducted in northern Karnataka, India, to identify the millet growing areas, particularly sorghum. The rhizobacteria from the sorghum ( Sorghum bicolor L.) were assessed for promoting seed germination using the paper towel method and classified based on their efficiency. The elite isolates were positive for indole-3-acetic acid (IAA), gibberellic acid (GA), phosphate, zinc oxide solubilization, and hydrogen cyanide (HCN) production. The test isolates were antagonistic to Macrophomina phaseolina and Fusarium sp. and inhibited completely. Further evaluation of the cultures on sorghum growth-promoting attributes under pot culture conditions showed that the plants inoculated with PG-152 ( Bacillus subtilis ) recorded the highest plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weight under ideal conditions of fertilization. Two isolates, namely, PG-152 and PG-197, performing superior under pot culture conditions, were identified as Bacillus subtilis and PG-197 as Enterobacter sp., respectively, using 16S rDNA analysis. The sequences were allowed to screen open reading frames (ORF) and found several ORFs in Enterobacter cloacae and Bacillus subtilis, respectively. This study found that the rhizosphere is vital for identifying prospective isolates for biocontrol and plant growth-improving microorganisms.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Elite isolates produced IAA, GA and HCN.

Taxon
sorghumSorghumt0
Interactor
elite isolatese0symbiont
Outcome
indole-3-acetic acid (IAA), gibberellic acid (GA), phosphate, zinc oxide solubilization, and hydrogen cyanide (HCN) productione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The elite isolates were positive for indole-3-acetic acid (IAA), gibberellic acid (GA), phosphate, zinc oxide solubilization, and hydrogen cyanide (HCN) production.”

✓ located verbatim in the source · abstract, characters 566–730 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Elite isolates solubilized phosphate and zinc oxide.

Taxon
sorghumSorghumt0
Interactor
elite isolatese0symbiont
Outcome
phosphate, zinc oxide solubilizatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The elite isolates were positive for indole-3-acetic acid (IAA), gibberellic acid (GA), phosphate, zinc oxide solubilization, and hydrogen cyanide (HCN) production.”

✓ located verbatim in the source · abstract, characters 566–730 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Test isolates completely inhibited Macrophomina phaseolina.

Taxon
sorghumSorghumt0
Interactor
Macrophomina phaseolinaMacrophomina phaseolinae0pathogen
Second interactor
test isolatese0biocontrol_agent
Outcome
Macrophomina phaseolinae0
Direction
inhibited completelye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Macrophomina phaseolina
Macrophomina phaseolina · InteractorEdward L. Barnard, Florida Department of Agriculture and Consumer Services · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The test isolates were antagonistic to Macrophomina phaseolina and Fusarium sp. and inhibited completely.”

✓ located verbatim in the source · abstract, characters 731–836 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Test isolates completely inhibited Fusarium sp.

Taxon
sorghumSorghumt0
Interactor
Fusarium sp.Fusariume0pathogen
Second interactor
test isolatese0biocontrol_agent
Outcome
Fusarium sp.e0
Direction
inhibited completelye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Fusarium
Fusarium · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e0

“The test isolates were antagonistic to Macrophomina phaseolina and Fusarium sp. and inhibited completely.”

✓ located verbatim in the source · abstract, characters 731–836 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PG-152 inoculation yielded the highest plant height, root dry weight and shoot dry weight under fertilization.

Taxon
sorghumSorghumt0
Treatment
inoculated with PG-152 ( Bacillus subtilis )e0
Interactor
Bacillus subtilisBacillus subtilise0symbiont
Outcome
plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weighte0
Direction
higheste0
Qualifier
under ideal conditions of fertilizatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the plants inoculated with PG-152 ( Bacillus subtilis ) recorded the highest plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weight under ideal conditions of fertilization.”

✓ located verbatim in the source · abstract, characters 952–1160 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PG-152 inoculation yielded the highest chlorophyll content under fertilization.

Taxon
sorghumSorghumt0
Treatment
inoculated with PG-152 ( Bacillus subtilis )e0
Interactor
Bacillus subtilisBacillus subtilise0symbiont
Outcome
chlorophyll contente0
Direction
higheste0
Qualifier
under ideal conditions of fertilizatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the plants inoculated with PG-152 ( Bacillus subtilis ) recorded the highest plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weight under ideal conditions of fertilization.”

✓ located verbatim in the source · abstract, characters 952–1160 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PG-152 inoculation yielded the highest total dry weight under fertilization.

Taxon
sorghumSorghumt0
Treatment
inoculated with PG-152 ( Bacillus subtilis )e0
Interactor
Bacillus subtilisBacillus subtilise0symbiont
Outcome
total dry weighte0
Direction
higheste0
Qualifier
under ideal conditions of fertilizatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the plants inoculated with PG-152 ( Bacillus subtilis ) recorded the highest plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weight under ideal conditions of fertilization.”

✓ located verbatim in the source · abstract, characters 952–1160 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

PG-152 and PG-197 were superior in pot culture.

Taxon
sorghumSorghumt0
Interactor
PG-152 and PG-197e0symbiont
Outcome
performing superiore0
Direction
superiore0
Setting
pot culture conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Two isolates, namely, PG-152 and PG-197, performing superior under pot culture conditions”

✓ located verbatim in the source · abstract, characters 1161–1250 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PG-152 was identified as Bacillus subtilis.

Taxon
sorghumSorghumt0
Interactor
Bacillus subtilisBacillus subtilise0symbiont
Outcome
identified as Bacillus subtilise0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“PG-152 and PG-197, performing superior under pot culture conditions, were identified as Bacillus subtilis and PG-197 as Enterobacter sp., respectively, using 16S rDNA analysis.”

✓ located verbatim in the source · abstract, characters 1183–1359 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PG-197 was identified as Enterobacter sp.

Taxon
sorghumSorghumt0
Interactor
Enterobacter sp.Enterobactere0symbiont
Outcome
Enterobacter sp.e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Enterobacter
Enterobacter · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e0

“PG-197 as Enterobacter sp.”

✓ located verbatim in the source · abstract, characters 1293–1319 · show in text ↑
t0

“sorghum”

✓ located verbatim in the source · title, characters 59–66 · show in text ↑

Bacillus subtilis · growth · SorghumPositive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. negative
See all 3 papers →

inoculation · physiology: pigments · SorghumPositive and negative results

11 papers: 9 positive, 2 negative or no effect

8 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

7 papers more in this group

See all 11 papers →

inoculation · growth · SorghumPositive and negative results

76 papers: 65 positive, 11 negative or no effect

64 papers in the same direction as this paper · 9 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

72 papers more in this group

See all 76 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.