Abstract
BACKGROUND: Castor is an important industrial raw material. Drought-induced oxidative stress leads to slow growth and decreased yields in castor. However, the mechanisms of drought-induced oxidative stress in castor remain unclear. Therefore, in this study, physiological, biochemical, and RNA-seq analyses were conducted on the roots of castor plants under PEG-6000 stress for 3 d and 7 d followed by 4 d of hydration. RESULTS: The photosynthetic rate of castor leaves was inhibited under PEG-6000 stress for 3 and 7 d. Biochemical analysis of castor roots stressed for 3 d and 7 d, and rehydrated for 4 d revealed that the activities of APX and CAT were highest after only 3 d of stress, whereas the activities of POD, GR, and SOD peaked after 7 d of stress. RNA-seq analysis revealed 2926, 1507, and 111 differentially expressed genes (DEGs) in the roots of castor plants under PEG-6000 stress for 3 d and 7 d and after 4 d of rehydration, respectively. GO analysis of the DEGs indicated significant enrichment in antioxidant activity. Furthermore, KEGG enrichment analysis of the DEGs revealed significantly enriched metabolic pathways, including glutathione metabolism, fatty acid metabolism, and plant hormone signal transduction. WGCNA identified the core genes PP2C39 and GA2ox4 in the navajowhite1 module, which was upregulated under PEG-6000 stress. On the basis of these results, we propose a model for the response to drought-induced oxidative stress in castor. CONCLUSIONS: This study provides valuable antioxidant gene resources, deepening our understanding of antioxidant regulation and paving the way for further molecular breeding of castor plants.
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Claims 9
Claim · model-written summary
PEG-6000 stress for 3 and 7 days inhibits the photosynthetic rate of castor leaves.
- Taxon
- castor
Ricinus communise1 - Organ / stage
- leavese1
- Treatment
- PEG-6000 stresse1
- Outcome
- photosynthetic ratee1
- Direction
- inhibitede1
- Qualifier
- for 3 and 7 de1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The photosynthetic rate of castor leaves was inhibited under PEG-6000 stress for 3 and 7 d”
Claim · model-written summary
Activities of APX and CAT in castor roots are highest after only 3 days of stress.
- Taxon
- castorRicinus communise2
- Organ / stage
- rootse2
- Treatment
- stressed for 3 de2
- Outcome
- activities of APX and CATe2
- Direction
- higheste2
- Qualifier
- after only 3 d of stresse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Biochemical analysis of castor roots stressed for 3 d and 7 d, and rehydrated for 4 d revealed that the activities of APX and CAT were highest after only 3 d of stress, whereas the activities of POD, GR, and SOD peaked after 7 d of stress”
Claim · model-written summary
Activities of POD, GR and SOD in castor roots peak after 7 days of stress.
- Taxon
- castorRicinus communise2
- Organ / stage
- rootse2
- Treatment
- after 7 d of stresse2
- Outcome
- activities of POD, GR, and SODe2
- Direction
- peakede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Biochemical analysis of castor roots stressed for 3 d and 7 d, and rehydrated for 4 d revealed that the activities of APX and CAT were highest after only 3 d of stress, whereas the activities of POD, GR, and SOD peaked after 7 d of stress”
Claim · model-written summary
Castor roots under PEG-6000 stress for 3 days show 2926 differentially expressed genes.
- Taxon
- castorRicinus communise3
- Organ / stage
- rootse3
- Treatment
- PEG-6000 stress for 3 de3
- Outcome
- differentially expressed genes (DEGs)e3
- Value
- 2926e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“RNA-seq analysis revealed 2926, 1507, and 111 differentially expressed genes (DEGs) in the roots of castor plants under PEG-6000 stress for 3 d and 7 d and after 4 d of rehydration, respectively”
Claim · model-written summary
Castor roots under PEG-6000 stress for 3 and 7 days show 1507 differentially expressed genes.
- Taxon
- castorRicinus communise3
- Organ / stage
- rootse3
- Treatment
- PEG-6000 stress for 3 d and 7 de3
- Outcome
- differentially expressed genes (DEGs)e3
- Value
- 1507e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“RNA-seq analysis revealed 2926, 1507, and 111 differentially expressed genes (DEGs) in the roots of castor plants under PEG-6000 stress for 3 d and 7 d and after 4 d of rehydration, respectively”
Claim · model-written summary
Castor roots after 4 days of rehydration show 111 differentially expressed genes.
- Taxon
- castorRicinus communise3
- Organ / stage
- rootse3
- Treatment
- after 4 d of rehydratione3
- Outcome
- differentially expressed genes (DEGs)e3
- Value
- 111e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“RNA-seq analysis revealed 2926, 1507, and 111 differentially expressed genes (DEGs) in the roots of castor plants under PEG-6000 stress for 3 d and 7 d and after 4 d of rehydration, respectively”
Claim · model-written summary
Differentially expressed genes of castor are significantly enriched in antioxidant activity.
- Taxon
- CastorRicinus communise0
- Outcome
- enrichment in antioxidant activitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Castor is an important industrial raw material”
e4“GO analysis of the DEGs indicated significant enrichment in antioxidant activity”
Claim · model-written summary
Differentially expressed genes of castor are significantly enriched in glutathione metabolism, fatty acid metabolism and plant hormone signal transduction.
- Taxon
- CastorRicinus communise0
- Outcome
- significantly enriched metabolic pathwayse5
- Qualifier
- glutathione metabolism, fatty acid metabolism, and plant hormone signal transductione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Castor is an important industrial raw material”
e5“KEGG enrichment analysis of the DEGs revealed significantly enriched metabolic pathways, including glutathione metabolism, fatty acid metabolism, and plant hormone signal transduction”
Claim · model-written summary
PP2C39 and GA2ox4 are core genes in the navajowhite1 module, which is upregulated under PEG-6000 stress in castor.
- Taxon
- CastorRicinus communise0
- Treatment
- PEG-6000 stresse6
- Gene
- PP2C39 and GA2ox4e6
- Outcome
- navajowhite1 modulee6
- Direction
- upregulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Castor is an important industrial raw material”
e6“WGCNA identified the core genes PP2C39 and GA2ox4 in the navajowhite1 module, which was upregulated under PEG-6000 stress”
The same questions in other papers
drought · physiology: photosynthesis · Ricinus communis (castor bean)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.